Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Ana Cristina
Data de Publicação: 2019
Outros Autores: Fontão, A., Leal, Marta, Soares da Silva, F. A. G., Coelho, Aires, Wan, Yizao, Dourado, Fernando, Gama, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/57494
Resumo: Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.nbt.2018.12.002.
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spelling Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost mediumBNC production optimizationCulture medium depthLow-cost substratesResponse surface methodology-central composite designSurface areaScience & TechnologySupplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.nbt.2018.12.002.This work aimed at the optimization of bacterial nanocellulose (BNC) production by static culture, using Komagataeibacter xylinus BPR 2001 (K. xylinus). Response surface methodology - central composite design was used to evaluate the effect of inexpensive and widely available nutrient sources, namely molasses, ethanol, corn steep liquor (CSL) and ammonium sulphate, on BNC production yield. The optimized parameters for maximum BNC production were % (m/v): molasses 5.38, CSL 1.91, ammonium sulphate 0.63, disodium phosphate 0.270, citric acid 0.115 and ethanol 1.38 % (v/v). The experimental and predicted maximum BNC production yields were 7.5±0.54g/L and 6.64±0.079g/L, respectively and the experimental and predicted maximum BNC productivity were 0.829±0.046g/L/day and 0.734±0.079g/L/day, after 9 days of static culture fermentation, at 30°C. The effect of surface area and culture medium depth on production yield and productivity were also studied. BNC dry mass production increased linearly with surface area, medium depth and fermentation time. So long as nutrients were still available in the culture media, BNC mass productivity was constant. The results show that a high BNC production yield can be obtained by static culture of K. xylinus BPR 2001 using a low-cost medium. These are promising conditions for the static industrial scale BNC production, since as compared to agitated bioreactors, higher productivities may be reached, while avoiding high capital and operating costs.The authors would like to acknowledge the Portuguese Foundation for Science and Technology (FCT) for the financial support of the PhD grant SFRH/BD/89547/2012 attributed to Ana Cristina Rodrigues, the financial support from project SkinChip: Disruptive cellulose-based microfluidic device for 3D skin modelling, PTDC/BBB-BIO/1889/2014. ThisstudywasalsosupportedbythePortugueseFoundationforScience and Technology (FCT) under the scope of the strategic funding of UID/ BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of NORTE 2020 - Programa Operacional Regional do Norte. The authors acknowledge COPAM Companhia Portuguesa de Amidos, S.A. (Portugal) and RAR Refinarias de Açúcar Reunidas, S.A. (Portugal) for kindly providing CSL and molasses, respectively.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoRodrigues, Ana CristinaFontão, A.Leal, MartaSoares da Silva, F. A. G.Coelho, AiresWan, YizaoDourado, FernandoGama, F. M.2019-03-252019-03-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/57494engRodrigues, Ana Cristina; Fontão, A.; Leal, Marta; Soares da Silva, F. A. G.; Coelho, Aires; Wan, Yizao; Dourado, Fernando; Gama, F. M., Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium. New Biotechnology, 49, 19-27, 20191871-67841871-678410.1016/j.nbt.2018.12.00230529474info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:31Zoai:repositorium.sdum.uminho.pt:1822/57494Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:39.720328Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
title Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
spellingShingle Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
Rodrigues, Ana Cristina
BNC production optimization
Culture medium depth
Low-cost substrates
Response surface methodology-central composite design
Surface area
Science & Technology
title_short Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
title_full Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
title_fullStr Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
title_full_unstemmed Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
title_sort Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
author Rodrigues, Ana Cristina
author_facet Rodrigues, Ana Cristina
Fontão, A.
Leal, Marta
Soares da Silva, F. A. G.
Coelho, Aires
Wan, Yizao
Dourado, Fernando
Gama, F. M.
author_role author
author2 Fontão, A.
Leal, Marta
Soares da Silva, F. A. G.
Coelho, Aires
Wan, Yizao
Dourado, Fernando
Gama, F. M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, Ana Cristina
Fontão, A.
Leal, Marta
Soares da Silva, F. A. G.
Coelho, Aires
Wan, Yizao
Dourado, Fernando
Gama, F. M.
dc.subject.por.fl_str_mv BNC production optimization
Culture medium depth
Low-cost substrates
Response surface methodology-central composite design
Surface area
Science & Technology
topic BNC production optimization
Culture medium depth
Low-cost substrates
Response surface methodology-central composite design
Surface area
Science & Technology
description Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.nbt.2018.12.002.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-25
2019-03-25T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/57494
url http://hdl.handle.net/1822/57494
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, Ana Cristina; Fontão, A.; Leal, Marta; Soares da Silva, F. A. G.; Coelho, Aires; Wan, Yizao; Dourado, Fernando; Gama, F. M., Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium. New Biotechnology, 49, 19-27, 2019
1871-6784
1871-6784
10.1016/j.nbt.2018.12.002
30529474
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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