Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/46851 |
Resumo: | Magnetotherapy has been receiving increased attention as an attractive strategy for modulating cell physiology directly at the site of injury, thereby providing the medical community with a safe and non- invasive therapy. Yet, how magnetic eld in uences tendon cells both at the cellular and molecular levels remains unclear. Thus, the in uence of a low-frequency static magnetic eld (2 Hz, 350 mT) on human tendon-derived cells was studied using di erent exposure times (4 and 8 h; short-term studies) and di erent regimens of exposure to an 8h-period of magnetic stimulation (continuous, every 24 h or every 48 h; long-term studies). Herein, 8 h stimulation in short-term studies signi cantly upregulated the expression of tendon-associated genes SCX, COL1A1, TNC and DCN (p < 0.05) and altered intracellular Ca2+ levels (p < 0.05). Additionally, every 24 h regimen of stimulation signi cantly upregulated COL1A1, COL3A1 and TNC at day 14 in comparison to control (p < 0.05), whereas continuous exposure di erentially regulated the release of the immunomodulatory cytokines IL-1β and IL-10 (p < 0.001) but only at day 7 in comparison to controls. Altogether, these results provide new insights on how low-frequency static magnetic eld ne-tune the behaviour of tendon cells according to the magnetic settings used, which we foresee to represent an interesting candidate to guide tendon regeneration. |
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Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesisContact-free therapyImmunomodulationMagnetotherapyRemote actuationTendon mechanotransductionTendon-related markersScience & TechnologyMagnetotherapy has been receiving increased attention as an attractive strategy for modulating cell physiology directly at the site of injury, thereby providing the medical community with a safe and non- invasive therapy. Yet, how magnetic eld in uences tendon cells both at the cellular and molecular levels remains unclear. Thus, the in uence of a low-frequency static magnetic eld (2 Hz, 350 mT) on human tendon-derived cells was studied using di erent exposure times (4 and 8 h; short-term studies) and di erent regimens of exposure to an 8h-period of magnetic stimulation (continuous, every 24 h or every 48 h; long-term studies). Herein, 8 h stimulation in short-term studies signi cantly upregulated the expression of tendon-associated genes SCX, COL1A1, TNC and DCN (p < 0.05) and altered intracellular Ca2+ levels (p < 0.05). Additionally, every 24 h regimen of stimulation signi cantly upregulated COL1A1, COL3A1 and TNC at day 14 in comparison to control (p < 0.05), whereas continuous exposure di erentially regulated the release of the immunomodulatory cytokines IL-1β and IL-10 (p < 0.001) but only at day 7 in comparison to controls. Altogether, these results provide new insights on how low-frequency static magnetic eld ne-tune the behaviour of tendon cells according to the magnetic settings used, which we foresee to represent an interesting candidate to guide tendon regeneration.info:eu-repo/semantics/publishedVersionNature Publishing GroupUniversidade do MinhoPesqueira, T.Almeida, Raquel CostaGomes, Manuela E.2017-092017-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/46851engPesqueira T., Costa-Almeida R., Gomes M. E. Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis, Scientific Reports, Vol. 7, pp. 10948, doi:10.1038/s41598-017-11253-6, 20172045-23222045-232210.1038/s41598-017-11253-628887547https://www.nature.com/articles/s41598-017-11253-6info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:04:05Zoai:repositorium.sdum.uminho.pt:1822/46851Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:54:17.520623Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis |
title |
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis |
spellingShingle |
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis Pesqueira, T. Contact-free therapy Immunomodulation Magnetotherapy Remote actuation Tendon mechanotransduction Tendon-related markers Science & Technology |
title_short |
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis |
title_full |
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis |
title_fullStr |
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis |
title_full_unstemmed |
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis |
title_sort |
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis |
author |
Pesqueira, T. |
author_facet |
Pesqueira, T. Almeida, Raquel Costa Gomes, Manuela E. |
author_role |
author |
author2 |
Almeida, Raquel Costa Gomes, Manuela E. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pesqueira, T. Almeida, Raquel Costa Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Contact-free therapy Immunomodulation Magnetotherapy Remote actuation Tendon mechanotransduction Tendon-related markers Science & Technology |
topic |
Contact-free therapy Immunomodulation Magnetotherapy Remote actuation Tendon mechanotransduction Tendon-related markers Science & Technology |
description |
Magnetotherapy has been receiving increased attention as an attractive strategy for modulating cell physiology directly at the site of injury, thereby providing the medical community with a safe and non- invasive therapy. Yet, how magnetic eld in uences tendon cells both at the cellular and molecular levels remains unclear. Thus, the in uence of a low-frequency static magnetic eld (2 Hz, 350 mT) on human tendon-derived cells was studied using di erent exposure times (4 and 8 h; short-term studies) and di erent regimens of exposure to an 8h-period of magnetic stimulation (continuous, every 24 h or every 48 h; long-term studies). Herein, 8 h stimulation in short-term studies signi cantly upregulated the expression of tendon-associated genes SCX, COL1A1, TNC and DCN (p < 0.05) and altered intracellular Ca2+ levels (p < 0.05). Additionally, every 24 h regimen of stimulation signi cantly upregulated COL1A1, COL3A1 and TNC at day 14 in comparison to control (p < 0.05), whereas continuous exposure di erentially regulated the release of the immunomodulatory cytokines IL-1β and IL-10 (p < 0.001) but only at day 7 in comparison to controls. Altogether, these results provide new insights on how low-frequency static magnetic eld ne-tune the behaviour of tendon cells according to the magnetic settings used, which we foresee to represent an interesting candidate to guide tendon regeneration. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09 2017-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/46851 |
url |
http://hdl.handle.net/1822/46851 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesqueira T., Costa-Almeida R., Gomes M. E. Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis, Scientific Reports, Vol. 7, pp. 10948, doi:10.1038/s41598-017-11253-6, 2017 2045-2322 2045-2322 10.1038/s41598-017-11253-6 28887547 https://www.nature.com/articles/s41598-017-11253-6 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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