Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/27471 https://doi.org/10.1016/j.neuroscience.2013.08.053 |
Resumo: | Diabetic retinopathy is one of the most frequent causes of blindness in adults in the Western countries. Although diabetic retinopathy is considered a vascular disease, several reports demonstrate that retinal neurons are also affected, leading to vision loss. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, has proven to be neuroprotective in several models of neurodegenerative diseases, including models of retinal degeneration. Since hyperglycemia is considered to play a central role in retinal cell dysfunction and degeneration, underlying the progression of diabetic retinopathy, the purpose of this study was to investigate the neuroprotective effects of TUDCA in rat retinal neurons exposed to elevated glucose concentration. We found that TUDCA markedly decreased cell death in cultured retinal neural cells induced by exposure to elevated glucose concentration. In addition, TUDCA partially prevented the release of apoptosis-inducing factor (AIF) from the mitochondria, as well as the subsequent accumulation of AIF in the nucleus. Biomarkers of oxidative stress, such as protein carbonyl groups and reactive oxygen species production, were markedly decreased after TUDCA treatment as compared to cells exposed to elevated glucose concentration alone. In conclusion, TUDCA protected retinal neural cell cultures from cell death induced by elevated glucose concentration, decreasing mito-nuclear translocation of AIF. The antioxidant properties of TUDCA might explain its cytoprotection. These findings may have relevance in the treatment of diabetic retinopathy patients. |
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Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucoseretinadiabetesdiabetic retinopathyTUDCAneural apoptosisDiabetic retinopathy is one of the most frequent causes of blindness in adults in the Western countries. Although diabetic retinopathy is considered a vascular disease, several reports demonstrate that retinal neurons are also affected, leading to vision loss. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, has proven to be neuroprotective in several models of neurodegenerative diseases, including models of retinal degeneration. Since hyperglycemia is considered to play a central role in retinal cell dysfunction and degeneration, underlying the progression of diabetic retinopathy, the purpose of this study was to investigate the neuroprotective effects of TUDCA in rat retinal neurons exposed to elevated glucose concentration. We found that TUDCA markedly decreased cell death in cultured retinal neural cells induced by exposure to elevated glucose concentration. In addition, TUDCA partially prevented the release of apoptosis-inducing factor (AIF) from the mitochondria, as well as the subsequent accumulation of AIF in the nucleus. Biomarkers of oxidative stress, such as protein carbonyl groups and reactive oxygen species production, were markedly decreased after TUDCA treatment as compared to cells exposed to elevated glucose concentration alone. In conclusion, TUDCA protected retinal neural cell cultures from cell death induced by elevated glucose concentration, decreasing mito-nuclear translocation of AIF. The antioxidant properties of TUDCA might explain its cytoprotection. These findings may have relevance in the treatment of diabetic retinopathy patients.Elsevier2013-12-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/27471http://hdl.handle.net/10316/27471https://doi.org/10.1016/j.neuroscience.2013.08.053engGASPAR, J. M. [et. al] - Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose. "Neuroscience". ISSN 0306-4522. Vol. 253 (2013) p. 380-3880306-4522http://www.sciencedirect.com/science/article/pii/S0306452213007495Gaspar, J. M.Martins, A.Cruz, R.Rodrigues, C. M. P.Ambrósio, A. F.Santiago, A. R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-07-21T09:59:13Zoai:estudogeral.uc.pt:10316/27471Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:53:39.677996Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose |
title |
Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose |
spellingShingle |
Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose Gaspar, J. M. retina diabetes diabetic retinopathy TUDCA neural apoptosis |
title_short |
Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose |
title_full |
Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose |
title_fullStr |
Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose |
title_full_unstemmed |
Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose |
title_sort |
Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose |
author |
Gaspar, J. M. |
author_facet |
Gaspar, J. M. Martins, A. Cruz, R. Rodrigues, C. M. P. Ambrósio, A. F. Santiago, A. R. |
author_role |
author |
author2 |
Martins, A. Cruz, R. Rodrigues, C. M. P. Ambrósio, A. F. Santiago, A. R. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gaspar, J. M. Martins, A. Cruz, R. Rodrigues, C. M. P. Ambrósio, A. F. Santiago, A. R. |
dc.subject.por.fl_str_mv |
retina diabetes diabetic retinopathy TUDCA neural apoptosis |
topic |
retina diabetes diabetic retinopathy TUDCA neural apoptosis |
description |
Diabetic retinopathy is one of the most frequent causes of blindness in adults in the Western countries. Although diabetic retinopathy is considered a vascular disease, several reports demonstrate that retinal neurons are also affected, leading to vision loss. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, has proven to be neuroprotective in several models of neurodegenerative diseases, including models of retinal degeneration. Since hyperglycemia is considered to play a central role in retinal cell dysfunction and degeneration, underlying the progression of diabetic retinopathy, the purpose of this study was to investigate the neuroprotective effects of TUDCA in rat retinal neurons exposed to elevated glucose concentration. We found that TUDCA markedly decreased cell death in cultured retinal neural cells induced by exposure to elevated glucose concentration. In addition, TUDCA partially prevented the release of apoptosis-inducing factor (AIF) from the mitochondria, as well as the subsequent accumulation of AIF in the nucleus. Biomarkers of oxidative stress, such as protein carbonyl groups and reactive oxygen species production, were markedly decreased after TUDCA treatment as compared to cells exposed to elevated glucose concentration alone. In conclusion, TUDCA protected retinal neural cell cultures from cell death induced by elevated glucose concentration, decreasing mito-nuclear translocation of AIF. The antioxidant properties of TUDCA might explain its cytoprotection. These findings may have relevance in the treatment of diabetic retinopathy patients. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/27471 http://hdl.handle.net/10316/27471 https://doi.org/10.1016/j.neuroscience.2013.08.053 |
url |
http://hdl.handle.net/10316/27471 https://doi.org/10.1016/j.neuroscience.2013.08.053 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
GASPAR, J. M. [et. al] - Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose. "Neuroscience". ISSN 0306-4522. Vol. 253 (2013) p. 380-388 0306-4522 http://www.sciencedirect.com/science/article/pii/S0306452213007495 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133822968659968 |