Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose

Detalhes bibliográficos
Autor(a) principal: Gaspar, J. M.
Data de Publicação: 2013
Outros Autores: Martins, A., Cruz, R., Rodrigues, C. M. P., Ambrósio, A. F., Santiago, A. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/27471
https://doi.org/10.1016/j.neuroscience.2013.08.053
Resumo: Diabetic retinopathy is one of the most frequent causes of blindness in adults in the Western countries. Although diabetic retinopathy is considered a vascular disease, several reports demonstrate that retinal neurons are also affected, leading to vision loss. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, has proven to be neuroprotective in several models of neurodegenerative diseases, including models of retinal degeneration. Since hyperglycemia is considered to play a central role in retinal cell dysfunction and degeneration, underlying the progression of diabetic retinopathy, the purpose of this study was to investigate the neuroprotective effects of TUDCA in rat retinal neurons exposed to elevated glucose concentration. We found that TUDCA markedly decreased cell death in cultured retinal neural cells induced by exposure to elevated glucose concentration. In addition, TUDCA partially prevented the release of apoptosis-inducing factor (AIF) from the mitochondria, as well as the subsequent accumulation of AIF in the nucleus. Biomarkers of oxidative stress, such as protein carbonyl groups and reactive oxygen species production, were markedly decreased after TUDCA treatment as compared to cells exposed to elevated glucose concentration alone. In conclusion, TUDCA protected retinal neural cell cultures from cell death induced by elevated glucose concentration, decreasing mito-nuclear translocation of AIF. The antioxidant properties of TUDCA might explain its cytoprotection. These findings may have relevance in the treatment of diabetic retinopathy patients.
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spelling Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucoseretinadiabetesdiabetic retinopathyTUDCAneural apoptosisDiabetic retinopathy is one of the most frequent causes of blindness in adults in the Western countries. Although diabetic retinopathy is considered a vascular disease, several reports demonstrate that retinal neurons are also affected, leading to vision loss. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, has proven to be neuroprotective in several models of neurodegenerative diseases, including models of retinal degeneration. Since hyperglycemia is considered to play a central role in retinal cell dysfunction and degeneration, underlying the progression of diabetic retinopathy, the purpose of this study was to investigate the neuroprotective effects of TUDCA in rat retinal neurons exposed to elevated glucose concentration. We found that TUDCA markedly decreased cell death in cultured retinal neural cells induced by exposure to elevated glucose concentration. In addition, TUDCA partially prevented the release of apoptosis-inducing factor (AIF) from the mitochondria, as well as the subsequent accumulation of AIF in the nucleus. Biomarkers of oxidative stress, such as protein carbonyl groups and reactive oxygen species production, were markedly decreased after TUDCA treatment as compared to cells exposed to elevated glucose concentration alone. In conclusion, TUDCA protected retinal neural cell cultures from cell death induced by elevated glucose concentration, decreasing mito-nuclear translocation of AIF. The antioxidant properties of TUDCA might explain its cytoprotection. These findings may have relevance in the treatment of diabetic retinopathy patients.Elsevier2013-12-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/27471http://hdl.handle.net/10316/27471https://doi.org/10.1016/j.neuroscience.2013.08.053engGASPAR, J. M. [et. al] - Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose. "Neuroscience". ISSN 0306-4522. Vol. 253 (2013) p. 380-3880306-4522http://www.sciencedirect.com/science/article/pii/S0306452213007495Gaspar, J. M.Martins, A.Cruz, R.Rodrigues, C. M. P.Ambrósio, A. F.Santiago, A. R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-07-21T09:59:13Zoai:estudogeral.uc.pt:10316/27471Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:53:39.677996Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
title Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
spellingShingle Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
Gaspar, J. M.
retina
diabetes
diabetic retinopathy
TUDCA
neural apoptosis
title_short Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
title_full Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
title_fullStr Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
title_full_unstemmed Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
title_sort Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose
author Gaspar, J. M.
author_facet Gaspar, J. M.
Martins, A.
Cruz, R.
Rodrigues, C. M. P.
Ambrósio, A. F.
Santiago, A. R.
author_role author
author2 Martins, A.
Cruz, R.
Rodrigues, C. M. P.
Ambrósio, A. F.
Santiago, A. R.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gaspar, J. M.
Martins, A.
Cruz, R.
Rodrigues, C. M. P.
Ambrósio, A. F.
Santiago, A. R.
dc.subject.por.fl_str_mv retina
diabetes
diabetic retinopathy
TUDCA
neural apoptosis
topic retina
diabetes
diabetic retinopathy
TUDCA
neural apoptosis
description Diabetic retinopathy is one of the most frequent causes of blindness in adults in the Western countries. Although diabetic retinopathy is considered a vascular disease, several reports demonstrate that retinal neurons are also affected, leading to vision loss. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, has proven to be neuroprotective in several models of neurodegenerative diseases, including models of retinal degeneration. Since hyperglycemia is considered to play a central role in retinal cell dysfunction and degeneration, underlying the progression of diabetic retinopathy, the purpose of this study was to investigate the neuroprotective effects of TUDCA in rat retinal neurons exposed to elevated glucose concentration. We found that TUDCA markedly decreased cell death in cultured retinal neural cells induced by exposure to elevated glucose concentration. In addition, TUDCA partially prevented the release of apoptosis-inducing factor (AIF) from the mitochondria, as well as the subsequent accumulation of AIF in the nucleus. Biomarkers of oxidative stress, such as protein carbonyl groups and reactive oxygen species production, were markedly decreased after TUDCA treatment as compared to cells exposed to elevated glucose concentration alone. In conclusion, TUDCA protected retinal neural cell cultures from cell death induced by elevated glucose concentration, decreasing mito-nuclear translocation of AIF. The antioxidant properties of TUDCA might explain its cytoprotection. These findings may have relevance in the treatment of diabetic retinopathy patients.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/27471
http://hdl.handle.net/10316/27471
https://doi.org/10.1016/j.neuroscience.2013.08.053
url http://hdl.handle.net/10316/27471
https://doi.org/10.1016/j.neuroscience.2013.08.053
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv GASPAR, J. M. [et. al] - Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose. "Neuroscience". ISSN 0306-4522. Vol. 253 (2013) p. 380-388
0306-4522
http://www.sciencedirect.com/science/article/pii/S0306452213007495
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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