Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.24950/rspmi.1497 |
Resumo: | Introduction: Rapid diagnostic testing for pneumonia has the presume potential to guide clinical decisions in reducing the use of broad-spectrum antibiotics. These benefits should be dependent upon the accuracy of these test. In order to evaluate the diagnostic yield and accuracy of the BioFire® FilmArray® pneumonia panel (BFFA) for pathogens identification in lower respiratory tract specimens, we conduct this retrospective study in a intensive care unit (ICU). Methods: In this study were included all patients (n = 104) admitted in our ICU with pneumonia who underwent BFFA test, between December 2019 and March 2021. Lower respiratory tract samples were collected and analysed simultaneously with BFPP and microbiological culture, as part of standard-of-care testing. The BFFA panel includes for 18 bacteria and 8 virus, that commonly cause pneumonia as well 7 antibiotic resistance genes, identified from tracheal aspirate or bronchial alveolar lavage (BAL) specimens. Results: This study included 104 patients, with an average age of 62 years. The BFFA was positive in 111/125 (88%) samples. The most common bacteria detected were Staphylococcus aureus (19%), Klebsiella pneumonia (14%) and Haemophilus influenza (10%). Discussion: There was strong concordance between BFFA and culture for detection of bacteria. In fact, most bacteria (76/106 [72%]) detected by BFFA were also identified in culture. In other hand, it is relevant that only one negative BFPP had an isolated agent in the cultural exam. Conclusion: Real-time specimen analysis with BFFA has the potential to identify bacterial and viral pathogens and theirs resistance markers, faster than traditional culture-based methods. In fact, the BFFA is a rapid and accurate method for detection of pathogens from lower respiratory tract infections with a 72% sensitivity and 100% specificity, in our studied population. |
id |
RCAP_349f642341c61773c1f51a966a374737 |
---|---|
oai_identifier_str |
oai:oai.revista.spmi.pt:article/1497 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory SpecimensPrecisão Diagnóstica do Painel de Pneumonia Bio Fire® Filmarray® na Deteção de Microorganismos em Secreções BrônquicasInfecções Respiratórias/diagnósticoPneumonia/diagnósticoTécnicas de Diagnóstico MolecularMolecular Diagnostic TechniquesPneumonia/ diagnosisRespiratory Tract Infections/diagnosisIntroduction: Rapid diagnostic testing for pneumonia has the presume potential to guide clinical decisions in reducing the use of broad-spectrum antibiotics. These benefits should be dependent upon the accuracy of these test. In order to evaluate the diagnostic yield and accuracy of the BioFire® FilmArray® pneumonia panel (BFFA) for pathogens identification in lower respiratory tract specimens, we conduct this retrospective study in a intensive care unit (ICU). Methods: In this study were included all patients (n = 104) admitted in our ICU with pneumonia who underwent BFFA test, between December 2019 and March 2021. Lower respiratory tract samples were collected and analysed simultaneously with BFPP and microbiological culture, as part of standard-of-care testing. The BFFA panel includes for 18 bacteria and 8 virus, that commonly cause pneumonia as well 7 antibiotic resistance genes, identified from tracheal aspirate or bronchial alveolar lavage (BAL) specimens. Results: This study included 104 patients, with an average age of 62 years. The BFFA was positive in 111/125 (88%) samples. The most common bacteria detected were Staphylococcus aureus (19%), Klebsiella pneumonia (14%) and Haemophilus influenza (10%). Discussion: There was strong concordance between BFFA and culture for detection of bacteria. In fact, most bacteria (76/106 [72%]) detected by BFFA were also identified in culture. In other hand, it is relevant that only one negative BFPP had an isolated agent in the cultural exam. Conclusion: Real-time specimen analysis with BFFA has the potential to identify bacterial and viral pathogens and theirs resistance markers, faster than traditional culture-based methods. In fact, the BFFA is a rapid and accurate method for detection of pathogens from lower respiratory tract infections with a 72% sensitivity and 100% specificity, in our studied population.Introdução: Um teste rápido de diagnostico de pneumonia tem o potencial de orientar decisões clínicas, como o início de antibioterapia, permitindo a redução do uso de antibióticos de amplo espectro. Contudo, a aceitação desses testes de diagnostico esta dependente da sua precisão. De forma a avaliar a assertividade diagnostica e a precisão do painel de pneumonia BioFire® FilmArray® (BFFA) na identificação de microorganismos em secreções brônquicas, realizamos este estudo retrospetivo, que decorreu no Serviço de Medicina Intensiva (SMI) do CMHT. Métodos: Neste estudo foram incluídos todos os doentes (n = 104) internados no nosso SMI com pneumonia e que realizaram o teste BFFA, entre dezembro de 2019 e março de 2021. As amostras do trato respiratório inferior foram colhidas e analisadas simultaneamente pelo BFFA e pela cultura microbiológica. O painel BFFA incluiu 18 bactérias e 8 vírus, que comumente causam pneumonia, bem como 7 genes de resistência a antibióticos, identificados a partir de amostras quer de aspirado traqueal, quer de lavado broncoalveolar (LBA). Resultados: Este estudo incluiu 104 pacientes, com idade media de 62 anos. O BFFA foi positivo em 111/125 (88%) das amostras colhidas. As bactérias mais comummente detetadas foram Staphylococcus aureus (19%), Klebsiella pneumonia (14%) e Haemophilus influenza (10%). Discussão: Foi possível inferir a forte concordância entre os resultados do BFFA e do exame cultural na deteção de bactérias. De facto, a maioria das bactérias (76/106 [72%]) detetadas pelo BFFA também foram identificadas na cultura. Por outro lado, e de realçar que de todos os BFFA negativos, apenas um teve isolamento de agente no exame cultural. Conclusão: A analise de amostras de secreções respiratórias em tempo real com o BFFA tem o potencial de identificar microorganismos, assim como marcadores de resistência, mais rapidamente do que os métodos tradicionais baseados no exame cultural. De facto, o BFFA e um método rápido e preciso para deteção de microorganismos causadores de infeções do trato respiratório inferior com proporção de concordância positiva de 72% e proporção de concordância negativa proxima de 100%, na população estudada.Sociedade Portuguesa de Medicina Interna2023-09-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.24950/rspmi.1497https://doi.org/10.24950/rspmi.1497Internal Medicine; Vol. 30 No. 3 (2023): Julho/Setembro; 152-156Medicina Interna; Vol. 30 N.º 3 (2023): Julho/Setembro; 152-1562183-99800872-671Xreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPporhttps://revista.spmi.pt/index.php/rpmi/article/view/1497https://revista.spmi.pt/index.php/rpmi/article/view/1497/1776Direitos de Autor (c) 2023 Medicina Internainfo:eu-repo/semantics/openAccessDinis, Ana ClaraCatorze, NunoNarciso, SofiaOliveira, Teresa2023-10-28T06:24:53Zoai:oai.revista.spmi.pt:article/1497Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:31:49.009414Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens Precisão Diagnóstica do Painel de Pneumonia Bio Fire® Filmarray® na Deteção de Microorganismos em Secreções Brônquicas |
title |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens |
spellingShingle |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens Dinis, Ana Clara Infecções Respiratórias/diagnóstico Pneumonia/diagnóstico Técnicas de Diagnóstico Molecular Molecular Diagnostic Techniques Pneumonia/ diagnosis Respiratory Tract Infections/diagnosis |
title_short |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens |
title_full |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens |
title_fullStr |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens |
title_full_unstemmed |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens |
title_sort |
Diagnostic Accuracy of Bio Fire® Film Array® Pneumonia Panel for Detection of Viral and Bacterial Pathogenski in Lower Respiratory Specimens |
author |
Dinis, Ana Clara |
author_facet |
Dinis, Ana Clara Catorze, Nuno Narciso, Sofia Oliveira, Teresa |
author_role |
author |
author2 |
Catorze, Nuno Narciso, Sofia Oliveira, Teresa |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Dinis, Ana Clara Catorze, Nuno Narciso, Sofia Oliveira, Teresa |
dc.subject.por.fl_str_mv |
Infecções Respiratórias/diagnóstico Pneumonia/diagnóstico Técnicas de Diagnóstico Molecular Molecular Diagnostic Techniques Pneumonia/ diagnosis Respiratory Tract Infections/diagnosis |
topic |
Infecções Respiratórias/diagnóstico Pneumonia/diagnóstico Técnicas de Diagnóstico Molecular Molecular Diagnostic Techniques Pneumonia/ diagnosis Respiratory Tract Infections/diagnosis |
description |
Introduction: Rapid diagnostic testing for pneumonia has the presume potential to guide clinical decisions in reducing the use of broad-spectrum antibiotics. These benefits should be dependent upon the accuracy of these test. In order to evaluate the diagnostic yield and accuracy of the BioFire® FilmArray® pneumonia panel (BFFA) for pathogens identification in lower respiratory tract specimens, we conduct this retrospective study in a intensive care unit (ICU). Methods: In this study were included all patients (n = 104) admitted in our ICU with pneumonia who underwent BFFA test, between December 2019 and March 2021. Lower respiratory tract samples were collected and analysed simultaneously with BFPP and microbiological culture, as part of standard-of-care testing. The BFFA panel includes for 18 bacteria and 8 virus, that commonly cause pneumonia as well 7 antibiotic resistance genes, identified from tracheal aspirate or bronchial alveolar lavage (BAL) specimens. Results: This study included 104 patients, with an average age of 62 years. The BFFA was positive in 111/125 (88%) samples. The most common bacteria detected were Staphylococcus aureus (19%), Klebsiella pneumonia (14%) and Haemophilus influenza (10%). Discussion: There was strong concordance between BFFA and culture for detection of bacteria. In fact, most bacteria (76/106 [72%]) detected by BFFA were also identified in culture. In other hand, it is relevant that only one negative BFPP had an isolated agent in the cultural exam. Conclusion: Real-time specimen analysis with BFFA has the potential to identify bacterial and viral pathogens and theirs resistance markers, faster than traditional culture-based methods. In fact, the BFFA is a rapid and accurate method for detection of pathogens from lower respiratory tract infections with a 72% sensitivity and 100% specificity, in our studied population. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.24950/rspmi.1497 https://doi.org/10.24950/rspmi.1497 |
url |
https://doi.org/10.24950/rspmi.1497 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revista.spmi.pt/index.php/rpmi/article/view/1497 https://revista.spmi.pt/index.php/rpmi/article/view/1497/1776 |
dc.rights.driver.fl_str_mv |
Direitos de Autor (c) 2023 Medicina Interna info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos de Autor (c) 2023 Medicina Interna |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Medicina Interna |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Medicina Interna |
dc.source.none.fl_str_mv |
Internal Medicine; Vol. 30 No. 3 (2023): Julho/Setembro; 152-156 Medicina Interna; Vol. 30 N.º 3 (2023): Julho/Setembro; 152-156 2183-9980 0872-671X reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133586560909313 |