Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/35265 |
Resumo: | Poly(vinylidene fluoride) (PVDF) and its main copolymers - poly(vinylidene fluoride-co-hexafluoropropene), P(VDF-HFP), and poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) - were processed by solvent casting at room temperature in the form of porous membranes. Copolymer membranes showed higher degree of porosity than PVDF, the average pore size being larger for P(VDF-TrFE) than for P(VDF-HFP) and PVDF. All membranes show high hydrophobicity with water contact angles in the range 94° to 115°, and electroactive beta phase contents above 90%. The adhesion and proliferation of both C2C12 myoblast and MC3T3-E1 pre-osteoblast cells on the membranes were investigated. It is demonstrated that PVDF membranes promote higher cell proliferation while P(VDF-HFP) membranes show the lowest proliferation for both kinds of cell. The proliferation on P(VDF-TrFE) membranes is cell dependent, higher for MC3T3-E1 cells but lower for C2C12 cells, related to the effect of the highly porous structure on the preferred morphology of each cell type, as the higher pore size and porosity of the P(VDF-TrFE) membrane induce cell elongation, which is preferred just by the C2C12 muscle cells. |
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Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applicationsPorous membranespoly(vinylidene fluoride)poly(vinylidene fluoride-co-trifluoroethylene)poly(vinylidene fluoride-co-hexafluoropropene)tissue engineeringCiências Médicas::Biotecnologia MédicaScience & TechnologyPoly(vinylidene fluoride) (PVDF) and its main copolymers - poly(vinylidene fluoride-co-hexafluoropropene), P(VDF-HFP), and poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) - were processed by solvent casting at room temperature in the form of porous membranes. Copolymer membranes showed higher degree of porosity than PVDF, the average pore size being larger for P(VDF-TrFE) than for P(VDF-HFP) and PVDF. All membranes show high hydrophobicity with water contact angles in the range 94° to 115°, and electroactive beta phase contents above 90%. The adhesion and proliferation of both C2C12 myoblast and MC3T3-E1 pre-osteoblast cells on the membranes were investigated. It is demonstrated that PVDF membranes promote higher cell proliferation while P(VDF-HFP) membranes show the lowest proliferation for both kinds of cell. The proliferation on P(VDF-TrFE) membranes is cell dependent, higher for MC3T3-E1 cells but lower for C2C12 cells, related to the effect of the highly porous structure on the preferred morphology of each cell type, as the higher pore size and porosity of the P(VDF-TrFE) membrane induce cell elongation, which is preferred just by the C2C12 muscle cells.Funded by FEDER funds through the “Programa Operacional Fatores de Competitividade e COMPETE” and by national funds arranged by FCT Fundação para a Ciência e a Tecnologia, project references PTDC/CTM-NAN/112574/2009 and PEST-C/FIS/UI607/2014. Funding from “MateproOptimizing Materials and Processes”, ref. NORTE-07-0124-FEDER-000037”, co-funded by the “Programa Operacional Regional do Norte” (ON.2 e O Novo Norte), under the “Quadro de Referência Estrategico Nacional” (QREN), through the “Fundo Europeu de Desenvolvimento Regional” (FEDER). FCT for the SFRH/BPD/90870/2012 grant.Elsevier B.V.Universidade do MinhoPereira, Eduardo Jorge NunesRibeiro, S.Ribeiro, C.Gombek, C. J.Gama, F. M.Gomes, A. C.Patterson, D. A.Lanceros-Méndez, S.2015-072015-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/35265engNunes-Pereira, J.; Ribeiro, Sylvie; Ribeiro, Clarisse; Gombek, C. J.; Gama, F. M.; Gomes, A. C.; Patterson, D. A.; Lanceros-Méndez, S., Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications. Polymer Testing, 44, 234-241, 20150142-94180142-941810.1016/j.polymertesting.2015.05.001http://www.sciencedirect.com/science/article/pii/S0142941815001063info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:39Zoai:repositorium.sdum.uminho.pt:1822/35265Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:56.672405Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications |
title |
Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications |
spellingShingle |
Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications Pereira, Eduardo Jorge Nunes Porous membranes poly(vinylidene fluoride) poly(vinylidene fluoride-co-trifluoroethylene) poly(vinylidene fluoride-co-hexafluoropropene) tissue engineering Ciências Médicas::Biotecnologia Médica Science & Technology |
title_short |
Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications |
title_full |
Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications |
title_fullStr |
Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications |
title_full_unstemmed |
Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications |
title_sort |
Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications |
author |
Pereira, Eduardo Jorge Nunes |
author_facet |
Pereira, Eduardo Jorge Nunes Ribeiro, S. Ribeiro, C. Gombek, C. J. Gama, F. M. Gomes, A. C. Patterson, D. A. Lanceros-Méndez, S. |
author_role |
author |
author2 |
Ribeiro, S. Ribeiro, C. Gombek, C. J. Gama, F. M. Gomes, A. C. Patterson, D. A. Lanceros-Méndez, S. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pereira, Eduardo Jorge Nunes Ribeiro, S. Ribeiro, C. Gombek, C. J. Gama, F. M. Gomes, A. C. Patterson, D. A. Lanceros-Méndez, S. |
dc.subject.por.fl_str_mv |
Porous membranes poly(vinylidene fluoride) poly(vinylidene fluoride-co-trifluoroethylene) poly(vinylidene fluoride-co-hexafluoropropene) tissue engineering Ciências Médicas::Biotecnologia Médica Science & Technology |
topic |
Porous membranes poly(vinylidene fluoride) poly(vinylidene fluoride-co-trifluoroethylene) poly(vinylidene fluoride-co-hexafluoropropene) tissue engineering Ciências Médicas::Biotecnologia Médica Science & Technology |
description |
Poly(vinylidene fluoride) (PVDF) and its main copolymers - poly(vinylidene fluoride-co-hexafluoropropene), P(VDF-HFP), and poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) - were processed by solvent casting at room temperature in the form of porous membranes. Copolymer membranes showed higher degree of porosity than PVDF, the average pore size being larger for P(VDF-TrFE) than for P(VDF-HFP) and PVDF. All membranes show high hydrophobicity with water contact angles in the range 94° to 115°, and electroactive beta phase contents above 90%. The adhesion and proliferation of both C2C12 myoblast and MC3T3-E1 pre-osteoblast cells on the membranes were investigated. It is demonstrated that PVDF membranes promote higher cell proliferation while P(VDF-HFP) membranes show the lowest proliferation for both kinds of cell. The proliferation on P(VDF-TrFE) membranes is cell dependent, higher for MC3T3-E1 cells but lower for C2C12 cells, related to the effect of the highly porous structure on the preferred morphology of each cell type, as the higher pore size and porosity of the P(VDF-TrFE) membrane induce cell elongation, which is preferred just by the C2C12 muscle cells. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07 2015-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/35265 |
url |
http://hdl.handle.net/1822/35265 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nunes-Pereira, J.; Ribeiro, Sylvie; Ribeiro, Clarisse; Gombek, C. J.; Gama, F. M.; Gomes, A. C.; Patterson, D. A.; Lanceros-Méndez, S., Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications. Polymer Testing, 44, 234-241, 2015 0142-9418 0142-9418 10.1016/j.polymertesting.2015.05.001 http://www.sciencedirect.com/science/article/pii/S0142941815001063 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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