Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications

Detalhes bibliográficos
Autor(a) principal: Pereira, Eduardo Jorge Nunes
Data de Publicação: 2015
Outros Autores: Ribeiro, S., Ribeiro, C., Gombek, C. J., Gama, F. M., Gomes, A. C., Patterson, D. A., Lanceros-Méndez, S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/35265
Resumo: Poly(vinylidene fluoride) (PVDF) and its main copolymers - poly(vinylidene fluoride-co-hexafluoropropene), P(VDF-HFP), and poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) - were processed by solvent casting at room temperature in the form of porous membranes. Copolymer membranes showed higher degree of porosity than PVDF, the average pore size being larger for P(VDF-TrFE) than for P(VDF-HFP) and PVDF. All membranes show high hydrophobicity with water contact angles in the range 94° to 115°, and electroactive beta phase contents above 90%. The adhesion and proliferation of both C2C12 myoblast and MC3T3-E1 pre-osteoblast cells on the membranes were investigated. It is demonstrated that PVDF membranes promote higher cell proliferation while P(VDF-HFP) membranes show the lowest proliferation for both kinds of cell. The proliferation on P(VDF-TrFE) membranes is cell dependent, higher for MC3T3-E1 cells but lower for C2C12 cells, related to the effect of the highly porous structure on the preferred morphology of each cell type, as the higher pore size and porosity of the P(VDF-TrFE) membrane induce cell elongation, which is preferred just by the C2C12 muscle cells.
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spelling Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applicationsPorous membranespoly(vinylidene fluoride)poly(vinylidene fluoride-co-trifluoroethylene)poly(vinylidene fluoride-co-hexafluoropropene)tissue engineeringCiências Médicas::Biotecnologia MédicaScience & TechnologyPoly(vinylidene fluoride) (PVDF) and its main copolymers - poly(vinylidene fluoride-co-hexafluoropropene), P(VDF-HFP), and poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) - were processed by solvent casting at room temperature in the form of porous membranes. Copolymer membranes showed higher degree of porosity than PVDF, the average pore size being larger for P(VDF-TrFE) than for P(VDF-HFP) and PVDF. All membranes show high hydrophobicity with water contact angles in the range 94° to 115°, and electroactive beta phase contents above 90%. The adhesion and proliferation of both C2C12 myoblast and MC3T3-E1 pre-osteoblast cells on the membranes were investigated. It is demonstrated that PVDF membranes promote higher cell proliferation while P(VDF-HFP) membranes show the lowest proliferation for both kinds of cell. The proliferation on P(VDF-TrFE) membranes is cell dependent, higher for MC3T3-E1 cells but lower for C2C12 cells, related to the effect of the highly porous structure on the preferred morphology of each cell type, as the higher pore size and porosity of the P(VDF-TrFE) membrane induce cell elongation, which is preferred just by the C2C12 muscle cells.Funded by FEDER funds through the “Programa Operacional Fatores de Competitividade e COMPETE” and by national funds arranged by FCT Fundação para a Ciência e a Tecnologia, project references PTDC/CTM-NAN/112574/2009 and PEST-C/FIS/UI607/2014. Funding from “MateproOptimizing Materials and Processes”, ref. NORTE-07-0124-FEDER-000037”, co-funded by the “Programa Operacional Regional do Norte” (ON.2 e O Novo Norte), under the “Quadro de Referência Estrategico Nacional” (QREN), through the “Fundo Europeu de Desenvolvimento Regional” (FEDER). FCT for the SFRH/BPD/90870/2012 grant.Elsevier B.V.Universidade do MinhoPereira, Eduardo Jorge NunesRibeiro, S.Ribeiro, C.Gombek, C. J.Gama, F. M.Gomes, A. C.Patterson, D. A.Lanceros-Méndez, S.2015-072015-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/35265engNunes-Pereira, J.; Ribeiro, Sylvie; Ribeiro, Clarisse; Gombek, C. J.; Gama, F. M.; Gomes, A. C.; Patterson, D. A.; Lanceros-Méndez, S., Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications. Polymer Testing, 44, 234-241, 20150142-94180142-941810.1016/j.polymertesting.2015.05.001http://www.sciencedirect.com/science/article/pii/S0142941815001063info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:39Zoai:repositorium.sdum.uminho.pt:1822/35265Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:56.672405Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
title Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
spellingShingle Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
Pereira, Eduardo Jorge Nunes
Porous membranes
poly(vinylidene fluoride)
poly(vinylidene fluoride-co-trifluoroethylene)
poly(vinylidene fluoride-co-hexafluoropropene)
tissue engineering
Ciências Médicas::Biotecnologia Médica
Science & Technology
title_short Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
title_full Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
title_fullStr Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
title_full_unstemmed Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
title_sort Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications
author Pereira, Eduardo Jorge Nunes
author_facet Pereira, Eduardo Jorge Nunes
Ribeiro, S.
Ribeiro, C.
Gombek, C. J.
Gama, F. M.
Gomes, A. C.
Patterson, D. A.
Lanceros-Méndez, S.
author_role author
author2 Ribeiro, S.
Ribeiro, C.
Gombek, C. J.
Gama, F. M.
Gomes, A. C.
Patterson, D. A.
Lanceros-Méndez, S.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pereira, Eduardo Jorge Nunes
Ribeiro, S.
Ribeiro, C.
Gombek, C. J.
Gama, F. M.
Gomes, A. C.
Patterson, D. A.
Lanceros-Méndez, S.
dc.subject.por.fl_str_mv Porous membranes
poly(vinylidene fluoride)
poly(vinylidene fluoride-co-trifluoroethylene)
poly(vinylidene fluoride-co-hexafluoropropene)
tissue engineering
Ciências Médicas::Biotecnologia Médica
Science & Technology
topic Porous membranes
poly(vinylidene fluoride)
poly(vinylidene fluoride-co-trifluoroethylene)
poly(vinylidene fluoride-co-hexafluoropropene)
tissue engineering
Ciências Médicas::Biotecnologia Médica
Science & Technology
description Poly(vinylidene fluoride) (PVDF) and its main copolymers - poly(vinylidene fluoride-co-hexafluoropropene), P(VDF-HFP), and poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) - were processed by solvent casting at room temperature in the form of porous membranes. Copolymer membranes showed higher degree of porosity than PVDF, the average pore size being larger for P(VDF-TrFE) than for P(VDF-HFP) and PVDF. All membranes show high hydrophobicity with water contact angles in the range 94° to 115°, and electroactive beta phase contents above 90%. The adhesion and proliferation of both C2C12 myoblast and MC3T3-E1 pre-osteoblast cells on the membranes were investigated. It is demonstrated that PVDF membranes promote higher cell proliferation while P(VDF-HFP) membranes show the lowest proliferation for both kinds of cell. The proliferation on P(VDF-TrFE) membranes is cell dependent, higher for MC3T3-E1 cells but lower for C2C12 cells, related to the effect of the highly porous structure on the preferred morphology of each cell type, as the higher pore size and porosity of the P(VDF-TrFE) membrane induce cell elongation, which is preferred just by the C2C12 muscle cells.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
2015-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/35265
url http://hdl.handle.net/1822/35265
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nunes-Pereira, J.; Ribeiro, Sylvie; Ribeiro, Clarisse; Gombek, C. J.; Gama, F. M.; Gomes, A. C.; Patterson, D. A.; Lanceros-Méndez, S., Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications. Polymer Testing, 44, 234-241, 2015
0142-9418
0142-9418
10.1016/j.polymertesting.2015.05.001
http://www.sciencedirect.com/science/article/pii/S0142941815001063
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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