Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/64330 |
Resumo: | The datasets generated for this study can be found in the 16S rRNA gene sequences submitted to the European Nucleotide Database (ENA) accession numbers LR655884, LR657299 to LR657303, PRJEB33623. The Whole Genome Shotgun project of Acetobacterium wieringae strain JM has been deposited at DDBJ/ENA/GenBank under the accession VSLA00000000. |
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Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jmcarbon monoxidesyngascarboxydotrophsacetogensAcetobacteriumScience & TechnologyThe datasets generated for this study can be found in the 16S rRNA gene sequences submitted to the European Nucleotide Database (ENA) accession numbers LR655884, LR657299 to LR657303, PRJEB33623. The Whole Genome Shotgun project of Acetobacterium wieringae strain JM has been deposited at DDBJ/ENA/GenBank under the accession VSLA00000000.Syngas is a substrate for the anaerobic bioproduction of fuels and valuable chemicals. In this study, anaerobic sludge was used for microbial enrichments with synthetic syngas and acetate as main substrates. The objectives of this study were to identify microbial networks (in enrichment cultures) for the conversion of syngas to added-value products, and to isolate robust, non-fastidious carboxydotrophs. Enrichment cultures produced methane and propionate, this last one an unusual product from syngas fermentation. A bacterium closely related to Acetobacterium wieringae was identified as most prevalent (87% relative abundance) in the enrichments. Methanospirillum sp. and propionate-producing bacteria clustering within the genera Anaerotignum and Pelobacter were also found. Further on, strain JM, was isolated and was found to be 99% identical (16S rRNA gene) to A. wieringae DSM 1911T. Digital DNA-DNA hybridization (dDDH) value between the genomes of strain JM and A. wieringae was 77.1%, indicating that strain JM is a new strain of A. wieringae. Strain JM can grow on carbon monoxide (100% CO, total pressure 170 kPa) without yeast extract or formate, producing mainly acetate. Remarkably, conversion of CO by strain JM showed shorter lag phase than in cultures of A. wieringae DSM 1911T, and about four times higher amount of CO was consumed in 7 days. Genome analysis suggests that strain JM uses the Wood-Ljungdahl pathway for the conversion of one carbon compounds (CO, formate, CO2/H2). Genes encoding bifurcational enzyme complexes with similarity to the bifurcational formate dehydrogenase (Fdh) of Clostridium autoethanogenum are present, and possibly relate to the higher tolerance to CO of strain JM compared to other Acetobacterium species. A. wieringae DSM 1911T grew on CO in medium containing 1 mM formate.The involved research was financially supported by Project NWO-GK-07 from the Netherlands Science Foundation (NWO), a Gravitation Grant (Project 024.002.002) of the Netherlands Ministry of Education, Culture and Science, and by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020–Programa Operacional Regional do Norte. The financial support from FCT and European Social Fund (POPH-QREN) through the grant PD/BD/128030/2016 given to AA, and through the project INNOVsyn – Innovative strategies for syngas fermentation (POCI-01-0145-FEDER-031377), are gratefully acknowledged.info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoArantes, Ana LuísaMoreira, João Paulo CarvalhoDiender, MartijnParshina, Sofiya N.Stams, A. J. M.Alves, M. M.Alves, J. I.Sousa, Diana Zita Machado2020-01-312020-01-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64330engArantes, Ana Luísa; Moreira, João; Diender, Martijn; Parshina, Sofiya N.; Stams, A. J. M.; Alves, M. Madalena; Alves, J. I.; Sousa, Diana Z., Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm. Frontiers in Microbiology, 11(58), 20201664-302X10.3389/fmicb.2020.00058http://journal.frontiersin.org/journal/microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:10Zoai:repositorium.sdum.uminho.pt:1822/64330Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:52:28.526085Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm |
| title |
Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm |
| spellingShingle |
Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm Arantes, Ana Luísa carbon monoxide syngas carboxydotrophs acetogens Acetobacterium Science & Technology |
| title_short |
Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm |
| title_full |
Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm |
| title_fullStr |
Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm |
| title_full_unstemmed |
Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm |
| title_sort |
Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm |
| author |
Arantes, Ana Luísa |
| author_facet |
Arantes, Ana Luísa Moreira, João Paulo Carvalho Diender, Martijn Parshina, Sofiya N. Stams, A. J. M. Alves, M. M. Alves, J. I. Sousa, Diana Zita Machado |
| author_role |
author |
| author2 |
Moreira, João Paulo Carvalho Diender, Martijn Parshina, Sofiya N. Stams, A. J. M. Alves, M. M. Alves, J. I. Sousa, Diana Zita Machado |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Arantes, Ana Luísa Moreira, João Paulo Carvalho Diender, Martijn Parshina, Sofiya N. Stams, A. J. M. Alves, M. M. Alves, J. I. Sousa, Diana Zita Machado |
| dc.subject.por.fl_str_mv |
carbon monoxide syngas carboxydotrophs acetogens Acetobacterium Science & Technology |
| topic |
carbon monoxide syngas carboxydotrophs acetogens Acetobacterium Science & Technology |
| description |
The datasets generated for this study can be found in the 16S rRNA gene sequences submitted to the European Nucleotide Database (ENA) accession numbers LR655884, LR657299 to LR657303, PRJEB33623. The Whole Genome Shotgun project of Acetobacterium wieringae strain JM has been deposited at DDBJ/ENA/GenBank under the accession VSLA00000000. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-01-31 2020-01-31T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/64330 |
| url |
http://hdl.handle.net/1822/64330 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Arantes, Ana Luísa; Moreira, João; Diender, Martijn; Parshina, Sofiya N.; Stams, A. J. M.; Alves, M. Madalena; Alves, J. I.; Sousa, Diana Z., Enrichment of anaerobic syngas-converting communities and isolation of a novel carboxydotrophic Acetobacterium wieringae strain jm. Frontiers in Microbiology, 11(58), 2020 1664-302X 10.3389/fmicb.2020.00058 http://journal.frontiersin.org/journal/microbiology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Frontiers Media S.A. |
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Frontiers Media S.A. |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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