Study of hepatocellular function in the murine model following hepatic artery selective clamping
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.4/1605 |
Resumo: | PURPOSE: To investigate the impact of selective hepatic artery clamping (SHAC) in hepatocellular function. METHODS: Three groups of Wistar male rats were subjected to SHAC ischemia period of 60min: Group A continuous SHAC were subjected to SHAC ischemia period of 60min, Group B intermittent SHAC of 30min with 5min of reperfusion and Group C intermittent SHAC of 15min with 5min of reperfusion. Animals without SHAC were included-Group D. To evaluate hepatocellular function blood markers and hepatic extraction function (HEF) using 99mTc-mebrofenin were performed before and after surgery. Flow cytometry was used to analyze oxidative stress and cell viability. RESULTS: A mortality rate of 7.6% in Group A was observed. HEF maintained normal values between the groups. Flow cytometry demonstrated no significant differences between the groups in viability, type of cell death as well as in the production of reactive oxygen species. CONCLUSIONS: The selective hepatic artery clamping compared to other clamping techniques results on increased cell viability and decreased hepatocyte death. The SHAC is a potential alternative to decrease per-operative bleeding while maintaining hepatocellular function. |
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Study of hepatocellular function in the murine model following hepatic artery selective clampingArtéria HepáticaRatosImagiologia com RadionuclídeosCirculação HepáticaPURPOSE: To investigate the impact of selective hepatic artery clamping (SHAC) in hepatocellular function. METHODS: Three groups of Wistar male rats were subjected to SHAC ischemia period of 60min: Group A continuous SHAC were subjected to SHAC ischemia period of 60min, Group B intermittent SHAC of 30min with 5min of reperfusion and Group C intermittent SHAC of 15min with 5min of reperfusion. Animals without SHAC were included-Group D. To evaluate hepatocellular function blood markers and hepatic extraction function (HEF) using 99mTc-mebrofenin were performed before and after surgery. Flow cytometry was used to analyze oxidative stress and cell viability. RESULTS: A mortality rate of 7.6% in Group A was observed. HEF maintained normal values between the groups. Flow cytometry demonstrated no significant differences between the groups in viability, type of cell death as well as in the production of reactive oxygen species. CONCLUSIONS: The selective hepatic artery clamping compared to other clamping techniques results on increased cell viability and decreased hepatocyte death. The SHAC is a potential alternative to decrease per-operative bleeding while maintaining hepatocellular function.RIHUCTralhão, JGAbrantes, AMGonçalves, ACHoti, ELaranjo, MMartins, ROliveiros, BCardoso, DSarmento-Ribeiro, ABBotelho, MFCastro e Sousa, F2013-12-12T12:33:53Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.4/1605engActa Cir Bras. 2013;28(9):657-63.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-11T14:22:52Zoai:rihuc.huc.min-saude.pt:10400.4/1605Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:04:05.598590Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Study of hepatocellular function in the murine model following hepatic artery selective clamping |
title |
Study of hepatocellular function in the murine model following hepatic artery selective clamping |
spellingShingle |
Study of hepatocellular function in the murine model following hepatic artery selective clamping Tralhão, JG Artéria Hepática Ratos Imagiologia com Radionuclídeos Circulação Hepática |
title_short |
Study of hepatocellular function in the murine model following hepatic artery selective clamping |
title_full |
Study of hepatocellular function in the murine model following hepatic artery selective clamping |
title_fullStr |
Study of hepatocellular function in the murine model following hepatic artery selective clamping |
title_full_unstemmed |
Study of hepatocellular function in the murine model following hepatic artery selective clamping |
title_sort |
Study of hepatocellular function in the murine model following hepatic artery selective clamping |
author |
Tralhão, JG |
author_facet |
Tralhão, JG Abrantes, AM Gonçalves, AC Hoti, E Laranjo, M Martins, R Oliveiros, B Cardoso, D Sarmento-Ribeiro, AB Botelho, MF Castro e Sousa, F |
author_role |
author |
author2 |
Abrantes, AM Gonçalves, AC Hoti, E Laranjo, M Martins, R Oliveiros, B Cardoso, D Sarmento-Ribeiro, AB Botelho, MF Castro e Sousa, F |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RIHUC |
dc.contributor.author.fl_str_mv |
Tralhão, JG Abrantes, AM Gonçalves, AC Hoti, E Laranjo, M Martins, R Oliveiros, B Cardoso, D Sarmento-Ribeiro, AB Botelho, MF Castro e Sousa, F |
dc.subject.por.fl_str_mv |
Artéria Hepática Ratos Imagiologia com Radionuclídeos Circulação Hepática |
topic |
Artéria Hepática Ratos Imagiologia com Radionuclídeos Circulação Hepática |
description |
PURPOSE: To investigate the impact of selective hepatic artery clamping (SHAC) in hepatocellular function. METHODS: Three groups of Wistar male rats were subjected to SHAC ischemia period of 60min: Group A continuous SHAC were subjected to SHAC ischemia period of 60min, Group B intermittent SHAC of 30min with 5min of reperfusion and Group C intermittent SHAC of 15min with 5min of reperfusion. Animals without SHAC were included-Group D. To evaluate hepatocellular function blood markers and hepatic extraction function (HEF) using 99mTc-mebrofenin were performed before and after surgery. Flow cytometry was used to analyze oxidative stress and cell viability. RESULTS: A mortality rate of 7.6% in Group A was observed. HEF maintained normal values between the groups. Flow cytometry demonstrated no significant differences between the groups in viability, type of cell death as well as in the production of reactive oxygen species. CONCLUSIONS: The selective hepatic artery clamping compared to other clamping techniques results on increased cell viability and decreased hepatocyte death. The SHAC is a potential alternative to decrease per-operative bleeding while maintaining hepatocellular function. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-12T12:33:53Z 2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.4/1605 |
url |
http://hdl.handle.net/10400.4/1605 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cir Bras. 2013;28(9):657-63. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131703239770112 |