Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/89500 |
Resumo: | This study aimed to investigate the potential of Brillouin spectroscopy as a non-contact and real-time tool for measuring the viscoelastic properties of agar culture media and using them to determine the percentages of their different components. The Brillouin spectrum of media samples with varying concentrations of different components was successfully acquired. A custom-designed refractometer was employed for assessing the refractive index of the gels. The study shows that the Brillouin spectrum and viscoelastic properties of the media vary in dependence on the concentration of their different components in a predictable and controllable way and that the variation is different for each of the additives tested. This approach has the advantage of correlating to the component percentages, providing a non-contact method for evaluating the evolution of the culture medium in real-time, without disturbing the growth of cells or microorganisms. The results of this study have the potential to encourage the design of new tools and methods targeting the prediction, measuring, and control of agar plate behavior during cell culture. This could include measuring and maintaining optimal humidity conditions for maintaining suitable agar plates for cell culture or monitoring cell growth by measuring the consumption of each substance. |
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Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopyAgarBrillouin spectroscopyViscoelastic propertiesScatteringPhotonicsBiomaterialEngenharia e Tecnologia::Engenharia Eletrotécnica, Eletrónica e InformáticaSaúde de qualidadeThis study aimed to investigate the potential of Brillouin spectroscopy as a non-contact and real-time tool for measuring the viscoelastic properties of agar culture media and using them to determine the percentages of their different components. The Brillouin spectrum of media samples with varying concentrations of different components was successfully acquired. A custom-designed refractometer was employed for assessing the refractive index of the gels. The study shows that the Brillouin spectrum and viscoelastic properties of the media vary in dependence on the concentration of their different components in a predictable and controllable way and that the variation is different for each of the additives tested. This approach has the advantage of correlating to the component percentages, providing a non-contact method for evaluating the evolution of the culture medium in real-time, without disturbing the growth of cells or microorganisms. The results of this study have the potential to encourage the design of new tools and methods targeting the prediction, measuring, and control of agar plate behavior during cell culture. This could include measuring and maintaining optimal humidity conditions for maintaining suitable agar plates for cell culture or monitoring cell growth by measuring the consumption of each substance.This work was supported by CMEMS-UMinho Strategic Project UIDB/04436/2020 and UIDP/04436/2020 and project MPhotonBiopsy, PTDC/FIS-OTI/1259/2020. Bruno Esteves was supported by FCT, PhD grant 2021.07239.BD.ElsevierUniversidade do MinhoEsteves, Bruno Miguel CarpinteiroRocha, A.Silva, Manuel Fernando RibeiroCorreia, J. H.Rodrigues, José Artur Oliveira2023-082023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/89500eng2589-152910.1016/j.mtla.2023.101858101858https://www.sciencedirect.com/science/article/pii/S2589152923001850?via%3Dihubinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-16T01:20:37Zoai:repositorium.sdum.uminho.pt:1822/89500Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:01:05.126690Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy |
title |
Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy |
spellingShingle |
Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy Esteves, Bruno Miguel Carpinteiro Agar Brillouin spectroscopy Viscoelastic properties Scattering Photonics Biomaterial Engenharia e Tecnologia::Engenharia Eletrotécnica, Eletrónica e Informática Saúde de qualidade |
title_short |
Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy |
title_full |
Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy |
title_fullStr |
Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy |
title_full_unstemmed |
Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy |
title_sort |
Non-contact determination of the viscoelastic properties of agar culture media by Brillouin spectroscopy |
author |
Esteves, Bruno Miguel Carpinteiro |
author_facet |
Esteves, Bruno Miguel Carpinteiro Rocha, A. Silva, Manuel Fernando Ribeiro Correia, J. H. Rodrigues, José Artur Oliveira |
author_role |
author |
author2 |
Rocha, A. Silva, Manuel Fernando Ribeiro Correia, J. H. Rodrigues, José Artur Oliveira |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Esteves, Bruno Miguel Carpinteiro Rocha, A. Silva, Manuel Fernando Ribeiro Correia, J. H. Rodrigues, José Artur Oliveira |
dc.subject.por.fl_str_mv |
Agar Brillouin spectroscopy Viscoelastic properties Scattering Photonics Biomaterial Engenharia e Tecnologia::Engenharia Eletrotécnica, Eletrónica e Informática Saúde de qualidade |
topic |
Agar Brillouin spectroscopy Viscoelastic properties Scattering Photonics Biomaterial Engenharia e Tecnologia::Engenharia Eletrotécnica, Eletrónica e Informática Saúde de qualidade |
description |
This study aimed to investigate the potential of Brillouin spectroscopy as a non-contact and real-time tool for measuring the viscoelastic properties of agar culture media and using them to determine the percentages of their different components. The Brillouin spectrum of media samples with varying concentrations of different components was successfully acquired. A custom-designed refractometer was employed for assessing the refractive index of the gels. The study shows that the Brillouin spectrum and viscoelastic properties of the media vary in dependence on the concentration of their different components in a predictable and controllable way and that the variation is different for each of the additives tested. This approach has the advantage of correlating to the component percentages, providing a non-contact method for evaluating the evolution of the culture medium in real-time, without disturbing the growth of cells or microorganisms. The results of this study have the potential to encourage the design of new tools and methods targeting the prediction, measuring, and control of agar plate behavior during cell culture. This could include measuring and maintaining optimal humidity conditions for maintaining suitable agar plates for cell culture or monitoring cell growth by measuring the consumption of each substance. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08 2023-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/89500 |
url |
https://hdl.handle.net/1822/89500 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2589-1529 10.1016/j.mtla.2023.101858 101858 https://www.sciencedirect.com/science/article/pii/S2589152923001850?via%3Dihub |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799138184922136576 |