Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme

Detalhes bibliográficos
Autor(a) principal: Franciscon, E.
Data de Publicação: 2010
Outros Autores: Piubeli, F., Fantinatti-Garboggini, F., Menezes, C. R., Silva, I. S., Paulo, Artur Cavaco, Grossman, M. J., Durrant, L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/14769
Resumo: Four different azo dyes were decolorized (color reduction >90%) by bacteria isolated from a textile wastewater effluent. Dye decolorizing was carried out under microaerobic conditions until completion, after which the aromatic amine concentration was determined. A laccase from Myceliophthora thermophila was used to catalyze coupling reactions of the aromatic amines produced from decolorizing the dyes. The reaction was carried out with stirring (100 rpm) in a weak acidic buffer solution (pH 5.0) at 45 °C for 3 days. The presence of aromatic amines in the samples after bacterial decolorizing confirmed the azo bond was reduced in the process. In addition, the UV–vis spectrum was shifted significantly after the sequential bacterial-laccase treatment also indicating a chemical transformation of the dyes. After laccase treatment the solutions formed colored soluble and precipitated products. The particles sizes making up the precipitates formed after laccase treatment varied between 105 and 483 nm as determined by Photon Correlation Spectroscopy (PCS). The laccase treatment also reduced the COD of the dye solutions by ∼20%. We show that successive bacterial-laccase treatment is effective in decolorized azo dyes by reduction of the azo bonds, and promoting coupling reactions between the aromatic amines formed. Promoting coupling reactions between the aromatic amines using enzymes may prove useful for the physical removal and reuse of these amines.
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spelling Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzymeAzo dyesBiodegradationBacteriaAromatic aminesPolymerizationLaccaseScience & TechnologyFour different azo dyes were decolorized (color reduction >90%) by bacteria isolated from a textile wastewater effluent. Dye decolorizing was carried out under microaerobic conditions until completion, after which the aromatic amine concentration was determined. A laccase from Myceliophthora thermophila was used to catalyze coupling reactions of the aromatic amines produced from decolorizing the dyes. The reaction was carried out with stirring (100 rpm) in a weak acidic buffer solution (pH 5.0) at 45 °C for 3 days. The presence of aromatic amines in the samples after bacterial decolorizing confirmed the azo bond was reduced in the process. In addition, the UV–vis spectrum was shifted significantly after the sequential bacterial-laccase treatment also indicating a chemical transformation of the dyes. After laccase treatment the solutions formed colored soluble and precipitated products. The particles sizes making up the precipitates formed after laccase treatment varied between 105 and 483 nm as determined by Photon Correlation Spectroscopy (PCS). The laccase treatment also reduced the COD of the dye solutions by ∼20%. We show that successive bacterial-laccase treatment is effective in decolorized azo dyes by reduction of the azo bonds, and promoting coupling reactions between the aromatic amines formed. Promoting coupling reactions between the aromatic amines using enzymes may prove useful for the physical removal and reuse of these amines.The authors would like to thank the Brazilian Foundation Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior of the Ministry of Education (CAPES) and the National Counsel for Technological and Scientific Development (CNPq) for providing grants to Elisangela Franciscon.ElsevierUniversidade do MinhoFranciscon, E.Piubeli, F.Fantinatti-Garboggini, F.Menezes, C. R.Silva, I. S.Paulo, Artur CavacoGrossman, M. J.Durrant, L. R.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/14769eng0141-022910.1016/j.enzmictec.2009.12.014info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:18Zoai:repositorium.sdum.uminho.pt:1822/14769Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:58:12.253268Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
title Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
spellingShingle Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
Franciscon, E.
Azo dyes
Biodegradation
Bacteria
Aromatic amines
Polymerization
Laccase
Science & Technology
title_short Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
title_full Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
title_fullStr Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
title_full_unstemmed Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
title_sort Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
author Franciscon, E.
author_facet Franciscon, E.
Piubeli, F.
Fantinatti-Garboggini, F.
Menezes, C. R.
Silva, I. S.
Paulo, Artur Cavaco
Grossman, M. J.
Durrant, L. R.
author_role author
author2 Piubeli, F.
Fantinatti-Garboggini, F.
Menezes, C. R.
Silva, I. S.
Paulo, Artur Cavaco
Grossman, M. J.
Durrant, L. R.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Franciscon, E.
Piubeli, F.
Fantinatti-Garboggini, F.
Menezes, C. R.
Silva, I. S.
Paulo, Artur Cavaco
Grossman, M. J.
Durrant, L. R.
dc.subject.por.fl_str_mv Azo dyes
Biodegradation
Bacteria
Aromatic amines
Polymerization
Laccase
Science & Technology
topic Azo dyes
Biodegradation
Bacteria
Aromatic amines
Polymerization
Laccase
Science & Technology
description Four different azo dyes were decolorized (color reduction >90%) by bacteria isolated from a textile wastewater effluent. Dye decolorizing was carried out under microaerobic conditions until completion, after which the aromatic amine concentration was determined. A laccase from Myceliophthora thermophila was used to catalyze coupling reactions of the aromatic amines produced from decolorizing the dyes. The reaction was carried out with stirring (100 rpm) in a weak acidic buffer solution (pH 5.0) at 45 °C for 3 days. The presence of aromatic amines in the samples after bacterial decolorizing confirmed the azo bond was reduced in the process. In addition, the UV–vis spectrum was shifted significantly after the sequential bacterial-laccase treatment also indicating a chemical transformation of the dyes. After laccase treatment the solutions formed colored soluble and precipitated products. The particles sizes making up the precipitates formed after laccase treatment varied between 105 and 483 nm as determined by Photon Correlation Spectroscopy (PCS). The laccase treatment also reduced the COD of the dye solutions by ∼20%. We show that successive bacterial-laccase treatment is effective in decolorized azo dyes by reduction of the azo bonds, and promoting coupling reactions between the aromatic amines formed. Promoting coupling reactions between the aromatic amines using enzymes may prove useful for the physical removal and reuse of these amines.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/14769
url http://hdl.handle.net/1822/14769
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0141-0229
10.1016/j.enzmictec.2009.12.014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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