Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/14769 |
Resumo: | Four different azo dyes were decolorized (color reduction >90%) by bacteria isolated from a textile wastewater effluent. Dye decolorizing was carried out under microaerobic conditions until completion, after which the aromatic amine concentration was determined. A laccase from Myceliophthora thermophila was used to catalyze coupling reactions of the aromatic amines produced from decolorizing the dyes. The reaction was carried out with stirring (100 rpm) in a weak acidic buffer solution (pH 5.0) at 45 °C for 3 days. The presence of aromatic amines in the samples after bacterial decolorizing confirmed the azo bond was reduced in the process. In addition, the UV–vis spectrum was shifted significantly after the sequential bacterial-laccase treatment also indicating a chemical transformation of the dyes. After laccase treatment the solutions formed colored soluble and precipitated products. The particles sizes making up the precipitates formed after laccase treatment varied between 105 and 483 nm as determined by Photon Correlation Spectroscopy (PCS). The laccase treatment also reduced the COD of the dye solutions by ∼20%. We show that successive bacterial-laccase treatment is effective in decolorized azo dyes by reduction of the azo bonds, and promoting coupling reactions between the aromatic amines formed. Promoting coupling reactions between the aromatic amines using enzymes may prove useful for the physical removal and reuse of these amines. |
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Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzymeAzo dyesBiodegradationBacteriaAromatic aminesPolymerizationLaccaseScience & TechnologyFour different azo dyes were decolorized (color reduction >90%) by bacteria isolated from a textile wastewater effluent. Dye decolorizing was carried out under microaerobic conditions until completion, after which the aromatic amine concentration was determined. A laccase from Myceliophthora thermophila was used to catalyze coupling reactions of the aromatic amines produced from decolorizing the dyes. The reaction was carried out with stirring (100 rpm) in a weak acidic buffer solution (pH 5.0) at 45 °C for 3 days. The presence of aromatic amines in the samples after bacterial decolorizing confirmed the azo bond was reduced in the process. In addition, the UV–vis spectrum was shifted significantly after the sequential bacterial-laccase treatment also indicating a chemical transformation of the dyes. After laccase treatment the solutions formed colored soluble and precipitated products. The particles sizes making up the precipitates formed after laccase treatment varied between 105 and 483 nm as determined by Photon Correlation Spectroscopy (PCS). The laccase treatment also reduced the COD of the dye solutions by ∼20%. We show that successive bacterial-laccase treatment is effective in decolorized azo dyes by reduction of the azo bonds, and promoting coupling reactions between the aromatic amines formed. Promoting coupling reactions between the aromatic amines using enzymes may prove useful for the physical removal and reuse of these amines.The authors would like to thank the Brazilian Foundation Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior of the Ministry of Education (CAPES) and the National Counsel for Technological and Scientific Development (CNPq) for providing grants to Elisangela Franciscon.ElsevierUniversidade do MinhoFranciscon, E.Piubeli, F.Fantinatti-Garboggini, F.Menezes, C. R.Silva, I. S.Paulo, Artur CavacoGrossman, M. J.Durrant, L. R.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/14769eng0141-022910.1016/j.enzmictec.2009.12.014info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:18Zoai:repositorium.sdum.uminho.pt:1822/14769Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:58:12.253268Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme |
title |
Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme |
spellingShingle |
Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme Franciscon, E. Azo dyes Biodegradation Bacteria Aromatic amines Polymerization Laccase Science & Technology |
title_short |
Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme |
title_full |
Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme |
title_fullStr |
Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme |
title_full_unstemmed |
Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme |
title_sort |
Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme |
author |
Franciscon, E. |
author_facet |
Franciscon, E. Piubeli, F. Fantinatti-Garboggini, F. Menezes, C. R. Silva, I. S. Paulo, Artur Cavaco Grossman, M. J. Durrant, L. R. |
author_role |
author |
author2 |
Piubeli, F. Fantinatti-Garboggini, F. Menezes, C. R. Silva, I. S. Paulo, Artur Cavaco Grossman, M. J. Durrant, L. R. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Franciscon, E. Piubeli, F. Fantinatti-Garboggini, F. Menezes, C. R. Silva, I. S. Paulo, Artur Cavaco Grossman, M. J. Durrant, L. R. |
dc.subject.por.fl_str_mv |
Azo dyes Biodegradation Bacteria Aromatic amines Polymerization Laccase Science & Technology |
topic |
Azo dyes Biodegradation Bacteria Aromatic amines Polymerization Laccase Science & Technology |
description |
Four different azo dyes were decolorized (color reduction >90%) by bacteria isolated from a textile wastewater effluent. Dye decolorizing was carried out under microaerobic conditions until completion, after which the aromatic amine concentration was determined. A laccase from Myceliophthora thermophila was used to catalyze coupling reactions of the aromatic amines produced from decolorizing the dyes. The reaction was carried out with stirring (100 rpm) in a weak acidic buffer solution (pH 5.0) at 45 °C for 3 days. The presence of aromatic amines in the samples after bacterial decolorizing confirmed the azo bond was reduced in the process. In addition, the UV–vis spectrum was shifted significantly after the sequential bacterial-laccase treatment also indicating a chemical transformation of the dyes. After laccase treatment the solutions formed colored soluble and precipitated products. The particles sizes making up the precipitates formed after laccase treatment varied between 105 and 483 nm as determined by Photon Correlation Spectroscopy (PCS). The laccase treatment also reduced the COD of the dye solutions by ∼20%. We show that successive bacterial-laccase treatment is effective in decolorized azo dyes by reduction of the azo bonds, and promoting coupling reactions between the aromatic amines formed. Promoting coupling reactions between the aromatic amines using enzymes may prove useful for the physical removal and reuse of these amines. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/14769 |
url |
http://hdl.handle.net/1822/14769 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0141-0229 10.1016/j.enzmictec.2009.12.014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132372358135808 |