Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/43900 |
Resumo: | Hybrid materials comprising zinc oxide and nanocellulose have been widely explored for a good number of applications, including wound healing/dressing related purposes. However, investigation of wound healing materials has been more focused on bacterial nanocellulose-based zinc oxide hybrid composites, which utilize a form of nanocellulose that is significantly more expensive to obtain due to its nature. This work aims the obtention of sustainable and safe nanocellulose-based zinc oxide hybrid composites as wound healing materials. For this purpose, in-situ synthesis’ conditions were explored, employing in-situ hydrothermal and sol-gel synthetic approaches, where the impact of various reactional parameters (reagents ratio; temperature; time; washing and drying methods) on the structural properties of obtained materials was explored. Successful obtention of all different materials was attested via Ultraviolet-Visible (UV-Vis) spectroscopy, which were characterized on a structural level by: FT-IR (Fourier Transform Infrared) spectroscopy (for study of nanocellulose characteristic vibrations); by Powder X-ray analysis (evaluation of diffraction patterns and crystallinity) and by electronic microscopy, through SEM (Scanning Electron Microsccopy) and TEM (Transmission Electron Microscopy). Obtained hybrid materials of nanocellulose/ZnO, that showed robustness and differentiating structural characteristics, were studied in vitro for determination of safe concentration through cytotoxicity tests performed on HaCaT cells, and their wound healing potential was also evaluated through in vitro assay. |
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Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiatorsHybrid materialNanocelluloseZinc oxideIn-situ synthesisWound healing potentialMaterial híbridoNanoceluloseÓxido de zincoSíntese in-situCicatrização de feridasDomínio/Área Científica::Ciências Médicas::Biotecnologia MédicaHybrid materials comprising zinc oxide and nanocellulose have been widely explored for a good number of applications, including wound healing/dressing related purposes. However, investigation of wound healing materials has been more focused on bacterial nanocellulose-based zinc oxide hybrid composites, which utilize a form of nanocellulose that is significantly more expensive to obtain due to its nature. This work aims the obtention of sustainable and safe nanocellulose-based zinc oxide hybrid composites as wound healing materials. For this purpose, in-situ synthesis’ conditions were explored, employing in-situ hydrothermal and sol-gel synthetic approaches, where the impact of various reactional parameters (reagents ratio; temperature; time; washing and drying methods) on the structural properties of obtained materials was explored. Successful obtention of all different materials was attested via Ultraviolet-Visible (UV-Vis) spectroscopy, which were characterized on a structural level by: FT-IR (Fourier Transform Infrared) spectroscopy (for study of nanocellulose characteristic vibrations); by Powder X-ray analysis (evaluation of diffraction patterns and crystallinity) and by electronic microscopy, through SEM (Scanning Electron Microsccopy) and TEM (Transmission Electron Microscopy). Obtained hybrid materials of nanocellulose/ZnO, that showed robustness and differentiating structural characteristics, were studied in vitro for determination of safe concentration through cytotoxicity tests performed on HaCaT cells, and their wound healing potential was also evaluated through in vitro assay.Materiais híbridos constituídos por óxido de zinco e nanocelulose têm sido amplamente explorados para um número de aplicações considerável, incluindo propósitos relacionados a cicatrização de feridas ou como curativos. No entanto, as investigações desse potencial têm sido particularmente direcionadas a compostos híbridos constituídos por óxido de zinco e nanocelulose bacteriana, que é significativamente mais cara de obter devido à sua natureza. Este trabalho visa a obtenção de compostos híbridos nanocelulose/ZnO que sejam seguros e sustentáveis para uso como materiais cicatrizantes. Para tal, foram exploradas condições de síntese in-situ, tendo-se usado os métodos hidrotérmico e sol-gel, onde se explorou o impato de diferentes parâmetros reacionais (rácio de reagentes; temperatura; tempo; métodos de lavagem e secagem) nas proprieadades estruturais dos materiais obtidos. A obtenção bem sucedida dos diferentes materiais foi atestada por espectroscopia de Ultravioleta-Visível (UV-Vis), os quais foram caraterizados a nível estrutural por: escpectroscopia de FT-IR (do inglês, Fourier Transform Infrared Spectroscopy) para estudo das vibrações caraterísticas da nanocelulose); por Raio-X de Pós (avaliação dos padrões de difração e cristalinidade) e por microscopia eletrónica, mediante utilização de SEM (do inglês, Scanning Electron Microsccopy) e TEM (do inglês, Transmission Electron Microscopy). Os materiais híbridos de nanocelulose/ZnO obtidos que demonstraram robustez e caraterísticas estruturais diferenciadores foram estudados in vitro para determinação da concentração segura a partir de testes citotóxicos efetuados em células HaCaT, e o seu potencial de cicatrização de feridas foi também avaliado a partir de ensaio in vitro.Pereira, Carla Patrícia FernandesRamos, Óscar Leandro da SilvaSilva, João Pedro AzevedoVeritati - Repositório Institucional da Universidade Católica PortuguesaMiranda, Clara Tavares2023-12-182023-112025-02-01T00:00:00Z2023-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.14/43900TID:203443063enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-13T01:34:25Zoai:repositorio.ucp.pt:10400.14/43900Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:37:58.211576Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators |
title |
Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators |
spellingShingle |
Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators Miranda, Clara Tavares Hybrid material Nanocellulose Zinc oxide In-situ synthesis Wound healing potential Material híbrido Nanocelulose Óxido de zinco Síntese in-situ Cicatrização de feridas Domínio/Área Científica::Ciências Médicas::Biotecnologia Médica |
title_short |
Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators |
title_full |
Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators |
title_fullStr |
Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators |
title_full_unstemmed |
Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators |
title_sort |
Preparation, characterization and biological evaluations of hybrid nanosystems as wound healing potentiators |
author |
Miranda, Clara Tavares |
author_facet |
Miranda, Clara Tavares |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pereira, Carla Patrícia Fernandes Ramos, Óscar Leandro da Silva Silva, João Pedro Azevedo Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Miranda, Clara Tavares |
dc.subject.por.fl_str_mv |
Hybrid material Nanocellulose Zinc oxide In-situ synthesis Wound healing potential Material híbrido Nanocelulose Óxido de zinco Síntese in-situ Cicatrização de feridas Domínio/Área Científica::Ciências Médicas::Biotecnologia Médica |
topic |
Hybrid material Nanocellulose Zinc oxide In-situ synthesis Wound healing potential Material híbrido Nanocelulose Óxido de zinco Síntese in-situ Cicatrização de feridas Domínio/Área Científica::Ciências Médicas::Biotecnologia Médica |
description |
Hybrid materials comprising zinc oxide and nanocellulose have been widely explored for a good number of applications, including wound healing/dressing related purposes. However, investigation of wound healing materials has been more focused on bacterial nanocellulose-based zinc oxide hybrid composites, which utilize a form of nanocellulose that is significantly more expensive to obtain due to its nature. This work aims the obtention of sustainable and safe nanocellulose-based zinc oxide hybrid composites as wound healing materials. For this purpose, in-situ synthesis’ conditions were explored, employing in-situ hydrothermal and sol-gel synthetic approaches, where the impact of various reactional parameters (reagents ratio; temperature; time; washing and drying methods) on the structural properties of obtained materials was explored. Successful obtention of all different materials was attested via Ultraviolet-Visible (UV-Vis) spectroscopy, which were characterized on a structural level by: FT-IR (Fourier Transform Infrared) spectroscopy (for study of nanocellulose characteristic vibrations); by Powder X-ray analysis (evaluation of diffraction patterns and crystallinity) and by electronic microscopy, through SEM (Scanning Electron Microsccopy) and TEM (Transmission Electron Microscopy). Obtained hybrid materials of nanocellulose/ZnO, that showed robustness and differentiating structural characteristics, were studied in vitro for determination of safe concentration through cytotoxicity tests performed on HaCaT cells, and their wound healing potential was also evaluated through in vitro assay. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-18 2023-11 2023-12-18T00:00:00Z 2025-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/43900 TID:203443063 |
url |
http://hdl.handle.net/10400.14/43900 |
identifier_str_mv |
TID:203443063 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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