Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/33774 |
Resumo: | In recent years, self-assembled biomaterials using peptides as building blocks have been developed as a solution for several medical challenges. Peptides offer a great diversity of biochemical properties and, through the combination of different amino acids, it is possible to produce a variety of peptide-based structures with specific proprieties with interest for regenerative medicine and tissue engineering. In the present project, novel hyaluronan base hydrogels obtained by supramolecular crosslinking with peptides were developed for biomedical application. Thus, different designs of peptides, containing a positively charged residue at one end and an aromatic residue at other end, were synthesized to form supramolecular interactions with hyaluronic acid (HA). The mass of the synthesised peptides was confirmed by Electrospray Ionization Mass Spectrometry (ESI-MS), their purity by High Performance Liquid Chromatography (HPLC) and their secondary structure by Circular Dichroism (CD) spectroscopy. In order to investigate gel formation, the peptides, in powder form and in solution of different concentrations at neutral and acidic pH, were combined with 1% and 2% w/v HA solutions of different molecular weights, following different strategies. Observation of gel formation was expected through electrostatic interactions between the positively charge residue of the peptide with HA (negatively charged) and π-π stacking between aromatic residues of the peptides. The resulted hydrogels were characterized by Scanning Electron Microscopy (SEM), revealing a network of nasnofibrous structures. The concentration of the HA solutions, different molecular weights HA and the peptide design showed to have an effect on the density, organization and packing of the fibers. |
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Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptidesDomínio/Área Científica::Engenharia e Tecnologia::Engenharia QuímicaIn recent years, self-assembled biomaterials using peptides as building blocks have been developed as a solution for several medical challenges. Peptides offer a great diversity of biochemical properties and, through the combination of different amino acids, it is possible to produce a variety of peptide-based structures with specific proprieties with interest for regenerative medicine and tissue engineering. In the present project, novel hyaluronan base hydrogels obtained by supramolecular crosslinking with peptides were developed for biomedical application. Thus, different designs of peptides, containing a positively charged residue at one end and an aromatic residue at other end, were synthesized to form supramolecular interactions with hyaluronic acid (HA). The mass of the synthesised peptides was confirmed by Electrospray Ionization Mass Spectrometry (ESI-MS), their purity by High Performance Liquid Chromatography (HPLC) and their secondary structure by Circular Dichroism (CD) spectroscopy. In order to investigate gel formation, the peptides, in powder form and in solution of different concentrations at neutral and acidic pH, were combined with 1% and 2% w/v HA solutions of different molecular weights, following different strategies. Observation of gel formation was expected through electrostatic interactions between the positively charge residue of the peptide with HA (negatively charged) and π-π stacking between aromatic residues of the peptides. The resulted hydrogels were characterized by Scanning Electron Microscopy (SEM), revealing a network of nasnofibrous structures. The concentration of the HA solutions, different molecular weights HA and the peptide design showed to have an effect on the density, organization and packing of the fibers.Nos últimos anos, biomateriais formados por auto-organização molecular constituídos por péptidos têm vindo a ser desenvolvidos como solução a vários desafios médicos. Os péptidos são interessantes unidades moleculares, uma vez que a partir da combinação de diferentes aminoácidos é possível obter biomateriais com propriedades específicas de bastante interesse em diversas áreas, como por exemplo, engenharia de tecidos e medicina regenerativa. Neste projeto, hidrogéis à base de ácido hialurónico foram desenvolvidos a partir de interações supramoleculares com diferentes péptidos, para aplicação biomédica. Como tal, péptidos com diferentes sequências, possuindo um resíduo carregado positivamente numa extremidade e um resíduo aromático na outra extremidade da sequência, foram sintetizados para formarem interações supramoleculares com o ácido hialurónico e interações entre os anéis aromáticos dos péptidos. A massa dos péptidos sintetizados foi confirmada por Espectrometria de Massa com Ionização por Electrospray, a sua pureza por Cromatografia Líquida de Alta Eficiência e a estrutura secundária por espectroscopia de dicroísmo circular. No sentido de investigar a formação de hidrogéis, os péptidos, em pó ou em soluções de diferentes concentrações a pH neutro e ácido, foram combinados com soluções de 1% e 2% de ácido hialurónico com diferentes massas moleculares, usando diferentes estratégias. Era esperada a formação de hidrogéis através de interações eletrostáticas entre os resíduos positivos do péptido e o ácido hialurónico (carga negativa), e ainda π-π stacking entre os anéis aromáticos dos resíduos dos péptidos. Os hidrogéis obtidos foram caracterizados por Microscopia de Varrimento Eletrónico, tendo revelado estruturas compostas por uma rede de nanofibras. A concentração da solução de ácido hialurónico e a sua massa molecular, bem como a sequência do péptido, mostraram ter efeito na densidade e organização das fibras.Azevedo, HelenaRadvar, ElhamVeritati - Repositório Institucional da Universidade Católica PortuguesaFaria, Ana Luísa de Oliveira Leite2021-06-21T13:00:08Z2017-05-3120172017-05-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.14/33774TID:201957990enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:39:20Zoai:repositorio.ucp.pt:10400.14/33774Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:27:22.849609Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides |
title |
Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides |
spellingShingle |
Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides Faria, Ana Luísa de Oliveira Leite Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
title_short |
Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides |
title_full |
Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides |
title_fullStr |
Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides |
title_full_unstemmed |
Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides |
title_sort |
Novel hyaluronan-based hydrogels obtained by supramolecular crosslinking with peptides |
author |
Faria, Ana Luísa de Oliveira Leite |
author_facet |
Faria, Ana Luísa de Oliveira Leite |
author_role |
author |
dc.contributor.none.fl_str_mv |
Azevedo, Helena Radvar, Elham Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Faria, Ana Luísa de Oliveira Leite |
dc.subject.por.fl_str_mv |
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
topic |
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
description |
In recent years, self-assembled biomaterials using peptides as building blocks have been developed as a solution for several medical challenges. Peptides offer a great diversity of biochemical properties and, through the combination of different amino acids, it is possible to produce a variety of peptide-based structures with specific proprieties with interest for regenerative medicine and tissue engineering. In the present project, novel hyaluronan base hydrogels obtained by supramolecular crosslinking with peptides were developed for biomedical application. Thus, different designs of peptides, containing a positively charged residue at one end and an aromatic residue at other end, were synthesized to form supramolecular interactions with hyaluronic acid (HA). The mass of the synthesised peptides was confirmed by Electrospray Ionization Mass Spectrometry (ESI-MS), their purity by High Performance Liquid Chromatography (HPLC) and their secondary structure by Circular Dichroism (CD) spectroscopy. In order to investigate gel formation, the peptides, in powder form and in solution of different concentrations at neutral and acidic pH, were combined with 1% and 2% w/v HA solutions of different molecular weights, following different strategies. Observation of gel formation was expected through electrostatic interactions between the positively charge residue of the peptide with HA (negatively charged) and π-π stacking between aromatic residues of the peptides. The resulted hydrogels were characterized by Scanning Electron Microscopy (SEM), revealing a network of nasnofibrous structures. The concentration of the HA solutions, different molecular weights HA and the peptide design showed to have an effect on the density, organization and packing of the fibers. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-31 2017 2017-05-31T00:00:00Z 2021-06-21T13:00:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/33774 TID:201957990 |
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http://hdl.handle.net/10400.14/33774 |
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TID:201957990 |
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eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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