Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/4743 https://doi.org/10.1016/j.domaniend.2005.07.007 |
Resumo: | Corpus luteum growth and endocrine function are closely dependent on the formation of new capillaries. The objectives of this study were to evaluate (i) tissue growth and microvascular development in the equine cyclic luteal structures; (ii) in vitro angiogenic activity of luteal tissues in response to luteotrophic (LH, PGE2) and luteolytic (PGF2[alpha]) hormones and (iii) to relate data to luteal endocrinological function. Our results show that microvascular density was increased in the early and mid luteal phase, followed by a fall in the late luteal phase and a further decrease in the corpus albicans. Hyperplasia of luteal tissue increased until the mid luteal phase and it was followed by tissue regression. Luteal explants were cultured with no hormone added, or with PGF2[alpha], LH, PGE2, LH + PGE2 or LH + PGF2[alpha]. Media conditioned by equine luteal tissue from different stages of the luteal phase were able to stimulate mitogenesis of bovine aortic endothelial cells (BAEC), suggesting the presence of angiogenic activity. No difference was observed among luteal structures on their mitogenic capacity, for any treatment used. Nevertheless, Late-CL conditioned-media with PGF2[alpha] showed a significant decrease in BAEC proliferation (p < 0.05) and LH + PGF2[alpha] a tendency to reduce mitogenesis. Thus, prostaglandin F2[alpha] may play a role on vascular regression of the CL during the late luteal phase in the mare. These data suggest that luteal angiogenesis and vascular regression in the mare are coordinated with the development of non-vascular tissue and might be regulated by many different factors. |
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Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycleCorpus luteumAngiogenesisVascularizationMitogenic factorsCorpus luteum growth and endocrine function are closely dependent on the formation of new capillaries. The objectives of this study were to evaluate (i) tissue growth and microvascular development in the equine cyclic luteal structures; (ii) in vitro angiogenic activity of luteal tissues in response to luteotrophic (LH, PGE2) and luteolytic (PGF2[alpha]) hormones and (iii) to relate data to luteal endocrinological function. Our results show that microvascular density was increased in the early and mid luteal phase, followed by a fall in the late luteal phase and a further decrease in the corpus albicans. Hyperplasia of luteal tissue increased until the mid luteal phase and it was followed by tissue regression. Luteal explants were cultured with no hormone added, or with PGF2[alpha], LH, PGE2, LH + PGE2 or LH + PGF2[alpha]. Media conditioned by equine luteal tissue from different stages of the luteal phase were able to stimulate mitogenesis of bovine aortic endothelial cells (BAEC), suggesting the presence of angiogenic activity. No difference was observed among luteal structures on their mitogenic capacity, for any treatment used. Nevertheless, Late-CL conditioned-media with PGF2[alpha] showed a significant decrease in BAEC proliferation (p < 0.05) and LH + PGF2[alpha] a tendency to reduce mitogenesis. Thus, prostaglandin F2[alpha] may play a role on vascular regression of the CL during the late luteal phase in the mare. These data suggest that luteal angiogenesis and vascular regression in the mare are coordinated with the development of non-vascular tissue and might be regulated by many different factors.http://www.sciencedirect.com/science/article/B6T62-4GV9B4H-2/1/2910d2927d48ce58f5dc2ca572bcd7c02006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/4743http://hdl.handle.net/10316/4743https://doi.org/10.1016/j.domaniend.2005.07.007engDomestic Animal Endocrinology. 30:4 (2006) 247-259Ferreira-Dias, G.Bravo, P. PintoMateus, L.Redmer, D. A.Medeiros, J. A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:48:57Zoai:estudogeral.uc.pt:10316/4743Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:34.567082Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle |
title |
Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle |
spellingShingle |
Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle Ferreira-Dias, G. Corpus luteum Angiogenesis Vascularization Mitogenic factors |
title_short |
Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle |
title_full |
Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle |
title_fullStr |
Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle |
title_full_unstemmed |
Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle |
title_sort |
Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle |
author |
Ferreira-Dias, G. |
author_facet |
Ferreira-Dias, G. Bravo, P. Pinto Mateus, L. Redmer, D. A. Medeiros, J. A. |
author_role |
author |
author2 |
Bravo, P. Pinto Mateus, L. Redmer, D. A. Medeiros, J. A. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ferreira-Dias, G. Bravo, P. Pinto Mateus, L. Redmer, D. A. Medeiros, J. A. |
dc.subject.por.fl_str_mv |
Corpus luteum Angiogenesis Vascularization Mitogenic factors |
topic |
Corpus luteum Angiogenesis Vascularization Mitogenic factors |
description |
Corpus luteum growth and endocrine function are closely dependent on the formation of new capillaries. The objectives of this study were to evaluate (i) tissue growth and microvascular development in the equine cyclic luteal structures; (ii) in vitro angiogenic activity of luteal tissues in response to luteotrophic (LH, PGE2) and luteolytic (PGF2[alpha]) hormones and (iii) to relate data to luteal endocrinological function. Our results show that microvascular density was increased in the early and mid luteal phase, followed by a fall in the late luteal phase and a further decrease in the corpus albicans. Hyperplasia of luteal tissue increased until the mid luteal phase and it was followed by tissue regression. Luteal explants were cultured with no hormone added, or with PGF2[alpha], LH, PGE2, LH + PGE2 or LH + PGF2[alpha]. Media conditioned by equine luteal tissue from different stages of the luteal phase were able to stimulate mitogenesis of bovine aortic endothelial cells (BAEC), suggesting the presence of angiogenic activity. No difference was observed among luteal structures on their mitogenic capacity, for any treatment used. Nevertheless, Late-CL conditioned-media with PGF2[alpha] showed a significant decrease in BAEC proliferation (p < 0.05) and LH + PGF2[alpha] a tendency to reduce mitogenesis. Thus, prostaglandin F2[alpha] may play a role on vascular regression of the CL during the late luteal phase in the mare. These data suggest that luteal angiogenesis and vascular regression in the mare are coordinated with the development of non-vascular tissue and might be regulated by many different factors. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/4743 http://hdl.handle.net/10316/4743 https://doi.org/10.1016/j.domaniend.2005.07.007 |
url |
http://hdl.handle.net/10316/4743 https://doi.org/10.1016/j.domaniend.2005.07.007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Domestic Animal Endocrinology. 30:4 (2006) 247-259 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133707822432256 |