A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium

Detalhes bibliográficos
Autor(a) principal: Silva, Nádia F. D.
Data de Publicação: 2015
Outros Autores: Magalhães, Júlia M. C. S., Oliva-Teles, Teresa, Delerue-Matos, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/7203
Resumo: Potentiometric detection with homemade polymeric membrane microelectrodes was coupled to a magnetic sandwich immunoassay for Salmonella typhimurium determination. Cadmium and sodium ion selective electrodes were used respectively as indicator and pseudo-reference electrodes and were prepared in pipette tips to allow potentiometric measurements in microliter sample volumes. In the proposed method, the concentration of S. typhimurium was proportional to the amount of cadmium released upon dissolution of a CdS nanoparticle labeled to the secondary detection antibody. The limit of detection was 2 cells per 100 μL. The immunomagnetic assay with potentiometric detection is suitable for sensitive and rapid (average total time per assay of 75 minutes) detection of S. typhimurium in milk samples. The proposed method is easy to perform, safe, sensitive, and low cost and has potential for in situ analysis.
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spelling A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimuriumPotentiometric detection with homemade polymeric membrane microelectrodes was coupled to a magnetic sandwich immunoassay for Salmonella typhimurium determination. Cadmium and sodium ion selective electrodes were used respectively as indicator and pseudo-reference electrodes and were prepared in pipette tips to allow potentiometric measurements in microliter sample volumes. In the proposed method, the concentration of S. typhimurium was proportional to the amount of cadmium released upon dissolution of a CdS nanoparticle labeled to the secondary detection antibody. The limit of detection was 2 cells per 100 μL. The immunomagnetic assay with potentiometric detection is suitable for sensitive and rapid (average total time per assay of 75 minutes) detection of S. typhimurium in milk samples. The proposed method is easy to perform, safe, sensitive, and low cost and has potential for in situ analysis.Royal Society of ChemistryRepositório Científico do Instituto Politécnico do PortoSilva, Nádia F. D.Magalhães, Júlia M. C. S.Oliva-Teles, TeresaDelerue-Matos, Cristina2015-12-21T16:19:59Z2015-042015-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/7203eng10.1039/C5AY00053Jinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:47:41Zoai:recipp.ipp.pt:10400.22/7203Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:27:46.031516Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
title A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
spellingShingle A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
Silva, Nádia F. D.
title_short A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
title_full A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
title_fullStr A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
title_full_unstemmed A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
title_sort A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
author Silva, Nádia F. D.
author_facet Silva, Nádia F. D.
Magalhães, Júlia M. C. S.
Oliva-Teles, Teresa
Delerue-Matos, Cristina
author_role author
author2 Magalhães, Júlia M. C. S.
Oliva-Teles, Teresa
Delerue-Matos, Cristina
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Silva, Nádia F. D.
Magalhães, Júlia M. C. S.
Oliva-Teles, Teresa
Delerue-Matos, Cristina
description Potentiometric detection with homemade polymeric membrane microelectrodes was coupled to a magnetic sandwich immunoassay for Salmonella typhimurium determination. Cadmium and sodium ion selective electrodes were used respectively as indicator and pseudo-reference electrodes and were prepared in pipette tips to allow potentiometric measurements in microliter sample volumes. In the proposed method, the concentration of S. typhimurium was proportional to the amount of cadmium released upon dissolution of a CdS nanoparticle labeled to the secondary detection antibody. The limit of detection was 2 cells per 100 μL. The immunomagnetic assay with potentiometric detection is suitable for sensitive and rapid (average total time per assay of 75 minutes) detection of S. typhimurium in milk samples. The proposed method is easy to perform, safe, sensitive, and low cost and has potential for in situ analysis.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-21T16:19:59Z
2015-04
2015-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/7203
url http://hdl.handle.net/10400.22/7203
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1039/C5AY00053J
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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