Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization

Detalhes bibliográficos
Autor(a) principal: Silva, Simone S.
Data de Publicação: 2020
Outros Autores: Gomes, J. M., Vale, Ana Catarina, Lu, Shenzhou, Reis, R. L., Kundu, Subhas C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/65404
Resumo: Silk protein fibroin (SF)-based matrices from non-mulberry, and mulberry silkworms are used for different applications in regenerative medicine. Silk fiber spun by the wild non-mulberry silkworm Antheraea pernyi (Ap) is also a promising biomedical material, due to the presence of the inherent tripeptide sequence of Arginine-Glycine-Aspartic acid (RGD) on the protein fibroin sequences. However, SF derived from the Ap cocoons still lacks exploitation in the healthcare field due to its poor solubility in the conventional solvents. This work addresses the application of green chemistry principles, namely the use of ionic liquids (ILs, 1-butyl-imidazolium acetate) and renewable resources such as Antheraea pernyi silk fibroin (ApSF) and chitin (Ch), for the fabrication of sponges from the blends of ApSF and Ch (APC). The formation of β-sheet in different contents during ApSF/Ch/IL was acquired by exposing gels to methanol/water and ethanol/water. The sponges were then obtained by freeze-drying. This approach promotes the formation of both stable and ordered ApSF/Ch-based sponges. The developed sponges show the suitable porosity and interconnectivity, appreciable swelling degree, and tuneable viscoelastic compressive properties for tissue engineering applications. Collectively, the structural properties of these ApSF/Ch-based sponges make them promising candidates for biomedical applications, namely cartilage regeneration.
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spelling Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterizationAntheraea pernyiBiomaterialsChitinIionic liquidsSilk FibroinSpongesionic liquidsScience & TechnologySilk protein fibroin (SF)-based matrices from non-mulberry, and mulberry silkworms are used for different applications in regenerative medicine. Silk fiber spun by the wild non-mulberry silkworm Antheraea pernyi (Ap) is also a promising biomedical material, due to the presence of the inherent tripeptide sequence of Arginine-Glycine-Aspartic acid (RGD) on the protein fibroin sequences. However, SF derived from the Ap cocoons still lacks exploitation in the healthcare field due to its poor solubility in the conventional solvents. This work addresses the application of green chemistry principles, namely the use of ionic liquids (ILs, 1-butyl-imidazolium acetate) and renewable resources such as Antheraea pernyi silk fibroin (ApSF) and chitin (Ch), for the fabrication of sponges from the blends of ApSF and Ch (APC). The formation of β-sheet in different contents during ApSF/Ch/IL was acquired by exposing gels to methanol/water and ethanol/water. The sponges were then obtained by freeze-drying. This approach promotes the formation of both stable and ordered ApSF/Ch-based sponges. The developed sponges show the suitable porosity and interconnectivity, appreciable swelling degree, and tuneable viscoelastic compressive properties for tissue engineering applications. Collectively, the structural properties of these ApSF/Ch-based sponges make them promising candidates for biomedical applications, namely cartilage regeneration.We thank the Portuguese FCT (PD/BD/135247/2017) to JG; Ph.D. programme in Advanced Therapies for Health (PATH) (PD/00169/2013) and R&D&I Structured Projects with reference NORTE-01-0145-FDER-000021 to SS; and European Union Framework Programme for Research and Innovation Horizon 2020 under grant agreement n◦ 668983—FoReCaST to SK and RR.Frontiers Media S.A.Universidade do MinhoSilva, Simone S.Gomes, J. M.Vale, Ana CatarinaLu, ShenzhouReis, R. L.Kundu, Subhas C2020-052020-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65404engSilva S. S., Gomes J. M., Vale A. C., Lu S., Reis R. L., Kundu S. C. Green Pathway for Processing Non-mulberry Antheraea pernyi Silk Fibroin/Chitin-Based Sponges: Biophysical and Biochemical Characterization, Frontiers in Materials, Vol. 7, pp. 1-9, doi:10.3389/fmats.2020.00135, 20202296-801610.3389/fmats.2020.00135https://www.frontiersin.org/articles/10.3389/fmats.2020.00135/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:04Zoai:repositorium.sdum.uminho.pt:1822/65404Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:41.507708Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
title Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
spellingShingle Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
Silva, Simone S.
Antheraea pernyi
Biomaterials
Chitin
Iionic liquids
Silk Fibroin
Sponges
ionic liquids
Science & Technology
title_short Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
title_full Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
title_fullStr Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
title_full_unstemmed Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
title_sort Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
author Silva, Simone S.
author_facet Silva, Simone S.
Gomes, J. M.
Vale, Ana Catarina
Lu, Shenzhou
Reis, R. L.
Kundu, Subhas C
author_role author
author2 Gomes, J. M.
Vale, Ana Catarina
Lu, Shenzhou
Reis, R. L.
Kundu, Subhas C
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Simone S.
Gomes, J. M.
Vale, Ana Catarina
Lu, Shenzhou
Reis, R. L.
Kundu, Subhas C
dc.subject.por.fl_str_mv Antheraea pernyi
Biomaterials
Chitin
Iionic liquids
Silk Fibroin
Sponges
ionic liquids
Science & Technology
topic Antheraea pernyi
Biomaterials
Chitin
Iionic liquids
Silk Fibroin
Sponges
ionic liquids
Science & Technology
description Silk protein fibroin (SF)-based matrices from non-mulberry, and mulberry silkworms are used for different applications in regenerative medicine. Silk fiber spun by the wild non-mulberry silkworm Antheraea pernyi (Ap) is also a promising biomedical material, due to the presence of the inherent tripeptide sequence of Arginine-Glycine-Aspartic acid (RGD) on the protein fibroin sequences. However, SF derived from the Ap cocoons still lacks exploitation in the healthcare field due to its poor solubility in the conventional solvents. This work addresses the application of green chemistry principles, namely the use of ionic liquids (ILs, 1-butyl-imidazolium acetate) and renewable resources such as Antheraea pernyi silk fibroin (ApSF) and chitin (Ch), for the fabrication of sponges from the blends of ApSF and Ch (APC). The formation of β-sheet in different contents during ApSF/Ch/IL was acquired by exposing gels to methanol/water and ethanol/water. The sponges were then obtained by freeze-drying. This approach promotes the formation of both stable and ordered ApSF/Ch-based sponges. The developed sponges show the suitable porosity and interconnectivity, appreciable swelling degree, and tuneable viscoelastic compressive properties for tissue engineering applications. Collectively, the structural properties of these ApSF/Ch-based sponges make them promising candidates for biomedical applications, namely cartilage regeneration.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
2020-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/65404
url http://hdl.handle.net/1822/65404
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva S. S., Gomes J. M., Vale A. C., Lu S., Reis R. L., Kundu S. C. Green Pathway for Processing Non-mulberry Antheraea pernyi Silk Fibroin/Chitin-Based Sponges: Biophysical and Biochemical Characterization, Frontiers in Materials, Vol. 7, pp. 1-9, doi:10.3389/fmats.2020.00135, 2020
2296-8016
10.3389/fmats.2020.00135
https://www.frontiersin.org/articles/10.3389/fmats.2020.00135/full
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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