Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/65404 |
Resumo: | Silk protein fibroin (SF)-based matrices from non-mulberry, and mulberry silkworms are used for different applications in regenerative medicine. Silk fiber spun by the wild non-mulberry silkworm Antheraea pernyi (Ap) is also a promising biomedical material, due to the presence of the inherent tripeptide sequence of Arginine-Glycine-Aspartic acid (RGD) on the protein fibroin sequences. However, SF derived from the Ap cocoons still lacks exploitation in the healthcare field due to its poor solubility in the conventional solvents. This work addresses the application of green chemistry principles, namely the use of ionic liquids (ILs, 1-butyl-imidazolium acetate) and renewable resources such as Antheraea pernyi silk fibroin (ApSF) and chitin (Ch), for the fabrication of sponges from the blends of ApSF and Ch (APC). The formation of β-sheet in different contents during ApSF/Ch/IL was acquired by exposing gels to methanol/water and ethanol/water. The sponges were then obtained by freeze-drying. This approach promotes the formation of both stable and ordered ApSF/Ch-based sponges. The developed sponges show the suitable porosity and interconnectivity, appreciable swelling degree, and tuneable viscoelastic compressive properties for tissue engineering applications. Collectively, the structural properties of these ApSF/Ch-based sponges make them promising candidates for biomedical applications, namely cartilage regeneration. |
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Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterizationAntheraea pernyiBiomaterialsChitinIionic liquidsSilk FibroinSpongesionic liquidsScience & TechnologySilk protein fibroin (SF)-based matrices from non-mulberry, and mulberry silkworms are used for different applications in regenerative medicine. Silk fiber spun by the wild non-mulberry silkworm Antheraea pernyi (Ap) is also a promising biomedical material, due to the presence of the inherent tripeptide sequence of Arginine-Glycine-Aspartic acid (RGD) on the protein fibroin sequences. However, SF derived from the Ap cocoons still lacks exploitation in the healthcare field due to its poor solubility in the conventional solvents. This work addresses the application of green chemistry principles, namely the use of ionic liquids (ILs, 1-butyl-imidazolium acetate) and renewable resources such as Antheraea pernyi silk fibroin (ApSF) and chitin (Ch), for the fabrication of sponges from the blends of ApSF and Ch (APC). The formation of β-sheet in different contents during ApSF/Ch/IL was acquired by exposing gels to methanol/water and ethanol/water. The sponges were then obtained by freeze-drying. This approach promotes the formation of both stable and ordered ApSF/Ch-based sponges. The developed sponges show the suitable porosity and interconnectivity, appreciable swelling degree, and tuneable viscoelastic compressive properties for tissue engineering applications. Collectively, the structural properties of these ApSF/Ch-based sponges make them promising candidates for biomedical applications, namely cartilage regeneration.We thank the Portuguese FCT (PD/BD/135247/2017) to JG; Ph.D. programme in Advanced Therapies for Health (PATH) (PD/00169/2013) and R&D&I Structured Projects with reference NORTE-01-0145-FDER-000021 to SS; and European Union Framework Programme for Research and Innovation Horizon 2020 under grant agreement n◦ 668983—FoReCaST to SK and RR.Frontiers Media S.A.Universidade do MinhoSilva, Simone S.Gomes, J. M.Vale, Ana CatarinaLu, ShenzhouReis, R. L.Kundu, Subhas C2020-052020-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65404engSilva S. S., Gomes J. M., Vale A. C., Lu S., Reis R. L., Kundu S. C. Green Pathway for Processing Non-mulberry Antheraea pernyi Silk Fibroin/Chitin-Based Sponges: Biophysical and Biochemical Characterization, Frontiers in Materials, Vol. 7, pp. 1-9, doi:10.3389/fmats.2020.00135, 20202296-801610.3389/fmats.2020.00135https://www.frontiersin.org/articles/10.3389/fmats.2020.00135/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:04Zoai:repositorium.sdum.uminho.pt:1822/65404Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:41.507708Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization |
title |
Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization |
spellingShingle |
Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization Silva, Simone S. Antheraea pernyi Biomaterials Chitin Iionic liquids Silk Fibroin Sponges ionic liquids Science & Technology |
title_short |
Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization |
title_full |
Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization |
title_fullStr |
Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization |
title_full_unstemmed |
Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization |
title_sort |
Green pathway for processing non-mulberry antheraea pernyi silk fibroin/chitin-based sponges: biophysical and biochemical characterization |
author |
Silva, Simone S. |
author_facet |
Silva, Simone S. Gomes, J. M. Vale, Ana Catarina Lu, Shenzhou Reis, R. L. Kundu, Subhas C |
author_role |
author |
author2 |
Gomes, J. M. Vale, Ana Catarina Lu, Shenzhou Reis, R. L. Kundu, Subhas C |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Simone S. Gomes, J. M. Vale, Ana Catarina Lu, Shenzhou Reis, R. L. Kundu, Subhas C |
dc.subject.por.fl_str_mv |
Antheraea pernyi Biomaterials Chitin Iionic liquids Silk Fibroin Sponges ionic liquids Science & Technology |
topic |
Antheraea pernyi Biomaterials Chitin Iionic liquids Silk Fibroin Sponges ionic liquids Science & Technology |
description |
Silk protein fibroin (SF)-based matrices from non-mulberry, and mulberry silkworms are used for different applications in regenerative medicine. Silk fiber spun by the wild non-mulberry silkworm Antheraea pernyi (Ap) is also a promising biomedical material, due to the presence of the inherent tripeptide sequence of Arginine-Glycine-Aspartic acid (RGD) on the protein fibroin sequences. However, SF derived from the Ap cocoons still lacks exploitation in the healthcare field due to its poor solubility in the conventional solvents. This work addresses the application of green chemistry principles, namely the use of ionic liquids (ILs, 1-butyl-imidazolium acetate) and renewable resources such as Antheraea pernyi silk fibroin (ApSF) and chitin (Ch), for the fabrication of sponges from the blends of ApSF and Ch (APC). The formation of β-sheet in different contents during ApSF/Ch/IL was acquired by exposing gels to methanol/water and ethanol/water. The sponges were then obtained by freeze-drying. This approach promotes the formation of both stable and ordered ApSF/Ch-based sponges. The developed sponges show the suitable porosity and interconnectivity, appreciable swelling degree, and tuneable viscoelastic compressive properties for tissue engineering applications. Collectively, the structural properties of these ApSF/Ch-based sponges make them promising candidates for biomedical applications, namely cartilage regeneration. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05 2020-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/65404 |
url |
http://hdl.handle.net/1822/65404 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva S. S., Gomes J. M., Vale A. C., Lu S., Reis R. L., Kundu S. C. Green Pathway for Processing Non-mulberry Antheraea pernyi Silk Fibroin/Chitin-Based Sponges: Biophysical and Biochemical Characterization, Frontiers in Materials, Vol. 7, pp. 1-9, doi:10.3389/fmats.2020.00135, 2020 2296-8016 10.3389/fmats.2020.00135 https://www.frontiersin.org/articles/10.3389/fmats.2020.00135/full |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
publisher.none.fl_str_mv |
Frontiers Media S.A. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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