Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/86429 |
Resumo: | The interactions between glioma cells and neurons are important for glioma progression but are rarely mimicked and recapitulated in in vitro three-dimensional (3D) models, which may affect the success rate of relevant drug research and development. In this study, an in vitro bioprinted 3D glioma model consisting of an outer hemispherical shell with neurons and an inner hemisphere with glioma cells is proposed to simulate the natural glioma. This model was produced by extrusion-based 3D bioprinting technology. The cells survival rate, morphology, and intercellular Ca2+ concentration studies were carried out up to 5 days of culturing. It was found that neurons could promote the proliferation of glioma cells around them, associate the morphological changes of glioma cells to be neuron-like, and increase the expression of intracellular Ca2+ of glioma cells. Conversely, the presence of glioma cells could maintain the neuronal survival rate and promote the neurite outgrowth. The results indicated that glioma cells and neurons facilitated each other implying a symbiotic pattern established between two types of cells during the early stage of glioma development, which were seldom found in the present artificial glioma models. The proposed bioprinted glioma model can mimic the natural microenvironment of glioma tissue, provide an in-depth understanding of cellâ cell interactions, and enable pathological and pharmacological studies of glioma. |
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Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactionsBiomimeticBioprintingGlioma modelIn vitro 3D model3D bioprintingGlioma cellsNeuronsThe interactions between glioma cells and neurons are important for glioma progression but are rarely mimicked and recapitulated in in vitro three-dimensional (3D) models, which may affect the success rate of relevant drug research and development. In this study, an in vitro bioprinted 3D glioma model consisting of an outer hemispherical shell with neurons and an inner hemisphere with glioma cells is proposed to simulate the natural glioma. This model was produced by extrusion-based 3D bioprinting technology. The cells survival rate, morphology, and intercellular Ca2+ concentration studies were carried out up to 5 days of culturing. It was found that neurons could promote the proliferation of glioma cells around them, associate the morphological changes of glioma cells to be neuron-like, and increase the expression of intracellular Ca2+ of glioma cells. Conversely, the presence of glioma cells could maintain the neuronal survival rate and promote the neurite outgrowth. The results indicated that glioma cells and neurons facilitated each other implying a symbiotic pattern established between two types of cells during the early stage of glioma development, which were seldom found in the present artificial glioma models. The proposed bioprinted glioma model can mimic the natural microenvironment of glioma tissue, provide an in-depth understanding of cellâ cell interactions, and enable pathological and pharmacological studies of glioma.The work was supported by the Program of the National Natural Science Foundation of China [52275291], [51675411], [81972359], the Fundamental Research Funds for the Central Universities, and the Youth Innovation Team of Shaanxi Universities.AccScience PublishingUniversidade do MinhoBai, LugeHao, ZhiyanWang, SenZhou, JiajiaYao, SiqiPei, NaZhu, HuiZhang, KunReis, R. L.Oliveira, Joaquim M.He, JiankangLi, DichenMao, XinggangWang, Ling2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86429engBai L., Hao Z., Wang S., Zhou J., Yao S., Pei N., Zhu H., Zhang K., Reis R. L., Oliveira J. M., He J., Li D., Mao X., Wang L. Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions, International Journal of Bioprinting, Vol. 9, Issue 4, doi:10.18063/ijb.715, 20232424-77232424-800210.18063/ijb.715https://ijb.whioce.com/index.php/int-j-bioprinting/article/viewFile/715/541info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-23T01:18:36Zoai:repositorium.sdum.uminho.pt:1822/86429Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:30:40.895611Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions |
title |
Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions |
spellingShingle |
Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions Bai, Luge Biomimetic Bioprinting Glioma model In vitro 3D model 3D bioprinting Glioma cells Neurons |
title_short |
Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions |
title_full |
Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions |
title_fullStr |
Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions |
title_full_unstemmed |
Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions |
title_sort |
Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions |
author |
Bai, Luge |
author_facet |
Bai, Luge Hao, Zhiyan Wang, Sen Zhou, Jiajia Yao, Siqi Pei, Na Zhu, Hui Zhang, Kun Reis, R. L. Oliveira, Joaquim M. He, Jiankang Li, Dichen Mao, Xinggang Wang, Ling |
author_role |
author |
author2 |
Hao, Zhiyan Wang, Sen Zhou, Jiajia Yao, Siqi Pei, Na Zhu, Hui Zhang, Kun Reis, R. L. Oliveira, Joaquim M. He, Jiankang Li, Dichen Mao, Xinggang Wang, Ling |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Bai, Luge Hao, Zhiyan Wang, Sen Zhou, Jiajia Yao, Siqi Pei, Na Zhu, Hui Zhang, Kun Reis, R. L. Oliveira, Joaquim M. He, Jiankang Li, Dichen Mao, Xinggang Wang, Ling |
dc.subject.por.fl_str_mv |
Biomimetic Bioprinting Glioma model In vitro 3D model 3D bioprinting Glioma cells Neurons |
topic |
Biomimetic Bioprinting Glioma model In vitro 3D model 3D bioprinting Glioma cells Neurons |
description |
The interactions between glioma cells and neurons are important for glioma progression but are rarely mimicked and recapitulated in in vitro three-dimensional (3D) models, which may affect the success rate of relevant drug research and development. In this study, an in vitro bioprinted 3D glioma model consisting of an outer hemispherical shell with neurons and an inner hemisphere with glioma cells is proposed to simulate the natural glioma. This model was produced by extrusion-based 3D bioprinting technology. The cells survival rate, morphology, and intercellular Ca2+ concentration studies were carried out up to 5 days of culturing. It was found that neurons could promote the proliferation of glioma cells around them, associate the morphological changes of glioma cells to be neuron-like, and increase the expression of intracellular Ca2+ of glioma cells. Conversely, the presence of glioma cells could maintain the neuronal survival rate and promote the neurite outgrowth. The results indicated that glioma cells and neurons facilitated each other implying a symbiotic pattern established between two types of cells during the early stage of glioma development, which were seldom found in the present artificial glioma models. The proposed bioprinted glioma model can mimic the natural microenvironment of glioma tissue, provide an in-depth understanding of cellâ cell interactions, and enable pathological and pharmacological studies of glioma. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03 2023-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/86429 |
url |
https://hdl.handle.net/1822/86429 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bai L., Hao Z., Wang S., Zhou J., Yao S., Pei N., Zhu H., Zhang K., Reis R. L., Oliveira J. M., He J., Li D., Mao X., Wang L. Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions, International Journal of Bioprinting, Vol. 9, Issue 4, doi:10.18063/ijb.715, 2023 2424-7723 2424-8002 10.18063/ijb.715 https://ijb.whioce.com/index.php/int-j-bioprinting/article/viewFile/715/541 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
AccScience Publishing |
publisher.none.fl_str_mv |
AccScience Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133575322271744 |