Self-assembled RGD dehydropeptide hydrogels for drug delivery applications
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/47150 |
Resumo: | Peptide-based self-assembled hydrogels have triggered remarkable research interest in recent years owing to their biocompatibility and biomimetic properties and responsiveness, which warrant many technological and biomedical applications. Dehydrodipeptides N-capped with naproxen emerged from our research as effective hydrogelators endowed with resistance to proteolysis. Dehydrodipeptide-based hydrogels are promising nanocarriers for drug delivery applications. In this work, we demonstrate that dehydrodipetide Npx-L-Ala-Z-DPhe-OH can be deployed as a minimalist hydrogelator module for synthesizing a gelating construct Npx-L-Ala-Z-DPhe-G-R-G-D-G-OH bearing a GRGDG adhesion motif. The self-assembly of the peptide construct and the drug delivery properties of the hydrogel were studied in this work. The peptide construct showed no toxicity towards a fibroblast cell line expressing the avb3 integrin. Docking studies suggest that the hydrogelator block does not interfere with the recognition of the RGD motif by the integrin receptor. The self-assembly seems to be directed by intermolecular naphthalene p–p stacking interactions, with the peptide backbone assuming a random coil conformation both in solution and in the gel phase. TEM and STEM imaging revealed that the hydrogel is made of entangled bundles of long thin fibres (width circa 23 nm). The hydrogel exhibits viscoelastic properties, thermo-reversibility and recovery after mechanical fluidization. FRET studies showed that curcumin incorporated into the hydrogel interacts non-covalently with the hydrogel fibrils. Delivery of curcumin from the hydrogel into Nile red loaded model membranes (SUVs) was demonstrated by FRET. Naproxen N-capped dehydrodipeptides are efficacious minimalist hydrogelator modules for obtaining hydrogels functionalized with peptide ligands for cell receptors. These hydrogels are potential nanocarriers for drug delivery. |
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Self-assembled RGD dehydropeptide hydrogels for drug delivery applicationsCiências Médicas::Biotecnologia MédicaCiências Naturais::Ciências QuímicasEngenharia e Tecnologia::NanotecnologiaScience & TechnologyPeptide-based self-assembled hydrogels have triggered remarkable research interest in recent years owing to their biocompatibility and biomimetic properties and responsiveness, which warrant many technological and biomedical applications. Dehydrodipeptides N-capped with naproxen emerged from our research as effective hydrogelators endowed with resistance to proteolysis. Dehydrodipeptide-based hydrogels are promising nanocarriers for drug delivery applications. In this work, we demonstrate that dehydrodipetide Npx-L-Ala-Z-DPhe-OH can be deployed as a minimalist hydrogelator module for synthesizing a gelating construct Npx-L-Ala-Z-DPhe-G-R-G-D-G-OH bearing a GRGDG adhesion motif. The self-assembly of the peptide construct and the drug delivery properties of the hydrogel were studied in this work. The peptide construct showed no toxicity towards a fibroblast cell line expressing the avb3 integrin. Docking studies suggest that the hydrogelator block does not interfere with the recognition of the RGD motif by the integrin receptor. The self-assembly seems to be directed by intermolecular naphthalene p–p stacking interactions, with the peptide backbone assuming a random coil conformation both in solution and in the gel phase. TEM and STEM imaging revealed that the hydrogel is made of entangled bundles of long thin fibres (width circa 23 nm). The hydrogel exhibits viscoelastic properties, thermo-reversibility and recovery after mechanical fluidization. FRET studies showed that curcumin incorporated into the hydrogel interacts non-covalently with the hydrogel fibrils. Delivery of curcumin from the hydrogel into Nile red loaded model membranes (SUVs) was demonstrated by FRET. Naproxen N-capped dehydrodipeptides are efficacious minimalist hydrogelator modules for obtaining hydrogels functionalized with peptide ligands for cell receptors. These hydrogels are potential nanocarriers for drug delivery.Thanks are due to Foundation for Science and Technology, FCT-Portugal, for financial support through Centre of Chemistry of University of Minho (CQ-UM) (projects UID/QUI/00686/ 2013 and UID/QUI/00686/2016) and Centre of Physics of Minho and Porto Universities, CF-UM-UP (project UID/FIS/04650/2013 and project UID/CEC/00319/2013). The NMR spectrometer Bruker Avance III 400 is part of the Portuguese NMR Network (Rede/1517/RMN/2005), which is also supported by FCT. Access to computing resources was funded by project NORTE-07-0162-FEDER-000086. TC acknowledges FCT PhD grant SFRH/BD/79195/2011.info:eu-repo/semantics/publishedVersionRoyal Society of ChemistryUniversidade do MinhoVilaça, HelenaCastro, Tarsila GabrielCosta, Fernando Miguel GonçalvesMelle-Franco, ManuelHilliou, L.Hamley, Ian W.Castanheira, Elisabete M. S.Martins, J. A. R.Ferreira, Paula M. T.20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/1822/47150eng2050-750X10.1039/c7tb01883e32264529info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:35ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Self-assembled RGD dehydropeptide hydrogels for drug delivery applications |
title |
Self-assembled RGD dehydropeptide hydrogels for drug delivery applications |
spellingShingle |
Self-assembled RGD dehydropeptide hydrogels for drug delivery applications Vilaça, Helena Ciências Médicas::Biotecnologia Médica Ciências Naturais::Ciências Químicas Engenharia e Tecnologia::Nanotecnologia Science & Technology |
title_short |
Self-assembled RGD dehydropeptide hydrogels for drug delivery applications |
title_full |
Self-assembled RGD dehydropeptide hydrogels for drug delivery applications |
title_fullStr |
Self-assembled RGD dehydropeptide hydrogels for drug delivery applications |
title_full_unstemmed |
Self-assembled RGD dehydropeptide hydrogels for drug delivery applications |
title_sort |
Self-assembled RGD dehydropeptide hydrogels for drug delivery applications |
author |
Vilaça, Helena |
author_facet |
Vilaça, Helena Castro, Tarsila Gabriel Costa, Fernando Miguel Gonçalves Melle-Franco, Manuel Hilliou, L. Hamley, Ian W. Castanheira, Elisabete M. S. Martins, J. A. R. Ferreira, Paula M. T. |
author_role |
author |
author2 |
Castro, Tarsila Gabriel Costa, Fernando Miguel Gonçalves Melle-Franco, Manuel Hilliou, L. Hamley, Ian W. Castanheira, Elisabete M. S. Martins, J. A. R. Ferreira, Paula M. T. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Vilaça, Helena Castro, Tarsila Gabriel Costa, Fernando Miguel Gonçalves Melle-Franco, Manuel Hilliou, L. Hamley, Ian W. Castanheira, Elisabete M. S. Martins, J. A. R. Ferreira, Paula M. T. |
dc.subject.por.fl_str_mv |
Ciências Médicas::Biotecnologia Médica Ciências Naturais::Ciências Químicas Engenharia e Tecnologia::Nanotecnologia Science & Technology |
topic |
Ciências Médicas::Biotecnologia Médica Ciências Naturais::Ciências Químicas Engenharia e Tecnologia::Nanotecnologia Science & Technology |
description |
Peptide-based self-assembled hydrogels have triggered remarkable research interest in recent years owing to their biocompatibility and biomimetic properties and responsiveness, which warrant many technological and biomedical applications. Dehydrodipeptides N-capped with naproxen emerged from our research as effective hydrogelators endowed with resistance to proteolysis. Dehydrodipeptide-based hydrogels are promising nanocarriers for drug delivery applications. In this work, we demonstrate that dehydrodipetide Npx-L-Ala-Z-DPhe-OH can be deployed as a minimalist hydrogelator module for synthesizing a gelating construct Npx-L-Ala-Z-DPhe-G-R-G-D-G-OH bearing a GRGDG adhesion motif. The self-assembly of the peptide construct and the drug delivery properties of the hydrogel were studied in this work. The peptide construct showed no toxicity towards a fibroblast cell line expressing the avb3 integrin. Docking studies suggest that the hydrogelator block does not interfere with the recognition of the RGD motif by the integrin receptor. The self-assembly seems to be directed by intermolecular naphthalene p–p stacking interactions, with the peptide backbone assuming a random coil conformation both in solution and in the gel phase. TEM and STEM imaging revealed that the hydrogel is made of entangled bundles of long thin fibres (width circa 23 nm). The hydrogel exhibits viscoelastic properties, thermo-reversibility and recovery after mechanical fluidization. FRET studies showed that curcumin incorporated into the hydrogel interacts non-covalently with the hydrogel fibrils. Delivery of curcumin from the hydrogel into Nile red loaded model membranes (SUVs) was demonstrated by FRET. Naproxen N-capped dehydrodipeptides are efficacious minimalist hydrogelator modules for obtaining hydrogels functionalized with peptide ligands for cell receptors. These hydrogels are potential nanocarriers for drug delivery. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/47150 |
url |
http://hdl.handle.net/1822/47150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2050-750X 10.1039/c7tb01883e 32264529 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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