Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/87288 |
Resumo: | Three-dimensional (3D) printing technology has become a popular tool to produce complex structures. It has great potential in the regenerative medicine field to produce customizable and reproducible scaffolds with high control of dimensions and porosity. This study was focused on the investigation of new biocompatible and biodegradable 3D-printed scaffolds with suitable mechanical properties to assist tendon and ligament regeneration. Polylactic acid (PLA) scaffolds were reinforced with 0.5 wt.% of functionalized graphite nanoplatelets decorated with silver nanoparticles ((f-EG)+Ag). The functionalization of graphene was carried out to strengthen the interface with the polymer. (f-EG)+Ag exhibited antibacterial properties against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>), an important feature for the healing process and prevention of bacterial infections. The scaffolds’ structure, biodegradation, and mechanical properties were assessed to confirm their suitability for tendon and ligamentregeneration. All scaffolds exhibited surface nanoroughness created during printing, which was increased by the filler presence. The wet state dynamic mechanical analysis proved that the incorporation of reinforcement led to an increase in the storage modulus, compared with neat PLA. The cytotoxicity assays using L929 fibroblasts showed that the scaffolds were biocompatible. The PLA+[(f-EG)+Ag] scaffolds were also loaded with human tendon-derived cells and showed their capability to maintain the tenogenic commitment with an increase in the gene expression of specific tendon/ligament-related markers. The results demonstrate the potential application of these new 3D-printed nanocomposite scaffolds for tendon and ligament regeneration. |
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Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites3D printingFunctionalized graphite nanoplateletsPLACompositesLigamentsTendonsThree-dimensional (3D) printing technology has become a popular tool to produce complex structures. It has great potential in the regenerative medicine field to produce customizable and reproducible scaffolds with high control of dimensions and porosity. This study was focused on the investigation of new biocompatible and biodegradable 3D-printed scaffolds with suitable mechanical properties to assist tendon and ligament regeneration. Polylactic acid (PLA) scaffolds were reinforced with 0.5 wt.% of functionalized graphite nanoplatelets decorated with silver nanoparticles ((f-EG)+Ag). The functionalization of graphene was carried out to strengthen the interface with the polymer. (f-EG)+Ag exhibited antibacterial properties against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>), an important feature for the healing process and prevention of bacterial infections. The scaffolds’ structure, biodegradation, and mechanical properties were assessed to confirm their suitability for tendon and ligamentregeneration. All scaffolds exhibited surface nanoroughness created during printing, which was increased by the filler presence. The wet state dynamic mechanical analysis proved that the incorporation of reinforcement led to an increase in the storage modulus, compared with neat PLA. The cytotoxicity assays using L929 fibroblasts showed that the scaffolds were biocompatible. The PLA+[(f-EG)+Ag] scaffolds were also loaded with human tendon-derived cells and showed their capability to maintain the tenogenic commitment with an increase in the gene expression of specific tendon/ligament-related markers. The results demonstrate the potential application of these new 3D-printed nanocomposite scaffolds for tendon and ligament regeneration.This research was funded by the Portuguese Foundation for Science and Technology (FCT) through the National Funds Reference UIDB/05256/2020 and UIDP/05256/2020, the FCT, EU and European Social Fund (FSE) through the Ph.D. Grant References SFRH/BD/138244/2018, COVID/BD/153245/2023, and SFRH/BD/143209/2019, the FCT in cooperation with the Northern Portugal Regional Coordination and Development Commission (CCDR-N) through the project “TERM RES Hub—Scientific Infrastructure for Tissue Engineering and Regenerative Medicine”, reference PINFRA/22190/2016 (Norte-01-0145-FEDER-022190), and the ERC CoG MagTendon (No. 772817).Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoSilva, Magda Sofia GonçalvesGomes, SusanaCorreia, Cátia Sofia PalmaPeixoto, DanielaVinhas, Carla Adriana AraújoRodrigues, Márcia T.Gomes, Manuela E.Covas, J. A.Paiva, Maria C.Alves, N. M.2023-09-082023-09-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/87288engSilva, M.; Gomes, S.; Correia, C.; Peixoto, D.; Vinhas, A.; Rodrigues, M.T.; Gomes, M.E.; Covas, J.A.; Paiva, M.C.; Alves, N.M. Biocompatible 3D-Printed Tendon/Ligament Scaffolds Based on Polylactic Acid/Graphite Nanoplatelet Composites. Nanomaterials 2023, 13, 2518. https://doi.org/10.3390/nano131825182079-499110.3390/nano131825182518https://www.mdpi.com/2079-4991/13/18/2518info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-25T01:17:03Zoai:repositorium.sdum.uminho.pt:1822/87288Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:19:39.474076Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites |
title |
Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites |
spellingShingle |
Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites Silva, Magda Sofia Gonçalves 3D printing Functionalized graphite nanoplatelets PLA Composites Ligaments Tendons |
title_short |
Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites |
title_full |
Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites |
title_fullStr |
Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites |
title_full_unstemmed |
Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites |
title_sort |
Biocompatible 3D-printed tendon/ligament scaffolds based on polylactic acid/graphite nanoplatelet composites |
author |
Silva, Magda Sofia Gonçalves |
author_facet |
Silva, Magda Sofia Gonçalves Gomes, Susana Correia, Cátia Sofia Palma Peixoto, Daniela Vinhas, Carla Adriana Araújo Rodrigues, Márcia T. Gomes, Manuela E. Covas, J. A. Paiva, Maria C. Alves, N. M. |
author_role |
author |
author2 |
Gomes, Susana Correia, Cátia Sofia Palma Peixoto, Daniela Vinhas, Carla Adriana Araújo Rodrigues, Márcia T. Gomes, Manuela E. Covas, J. A. Paiva, Maria C. Alves, N. M. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Magda Sofia Gonçalves Gomes, Susana Correia, Cátia Sofia Palma Peixoto, Daniela Vinhas, Carla Adriana Araújo Rodrigues, Márcia T. Gomes, Manuela E. Covas, J. A. Paiva, Maria C. Alves, N. M. |
dc.subject.por.fl_str_mv |
3D printing Functionalized graphite nanoplatelets PLA Composites Ligaments Tendons |
topic |
3D printing Functionalized graphite nanoplatelets PLA Composites Ligaments Tendons |
description |
Three-dimensional (3D) printing technology has become a popular tool to produce complex structures. It has great potential in the regenerative medicine field to produce customizable and reproducible scaffolds with high control of dimensions and porosity. This study was focused on the investigation of new biocompatible and biodegradable 3D-printed scaffolds with suitable mechanical properties to assist tendon and ligament regeneration. Polylactic acid (PLA) scaffolds were reinforced with 0.5 wt.% of functionalized graphite nanoplatelets decorated with silver nanoparticles ((f-EG)+Ag). The functionalization of graphene was carried out to strengthen the interface with the polymer. (f-EG)+Ag exhibited antibacterial properties against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>), an important feature for the healing process and prevention of bacterial infections. The scaffolds’ structure, biodegradation, and mechanical properties were assessed to confirm their suitability for tendon and ligamentregeneration. All scaffolds exhibited surface nanoroughness created during printing, which was increased by the filler presence. The wet state dynamic mechanical analysis proved that the incorporation of reinforcement led to an increase in the storage modulus, compared with neat PLA. The cytotoxicity assays using L929 fibroblasts showed that the scaffolds were biocompatible. The PLA+[(f-EG)+Ag] scaffolds were also loaded with human tendon-derived cells and showed their capability to maintain the tenogenic commitment with an increase in the gene expression of specific tendon/ligament-related markers. The results demonstrate the potential application of these new 3D-printed nanocomposite scaffolds for tendon and ligament regeneration. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-08 2023-09-08T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/87288 |
url |
https://hdl.handle.net/1822/87288 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, M.; Gomes, S.; Correia, C.; Peixoto, D.; Vinhas, A.; Rodrigues, M.T.; Gomes, M.E.; Covas, J.A.; Paiva, M.C.; Alves, N.M. Biocompatible 3D-Printed Tendon/Ligament Scaffolds Based on Polylactic Acid/Graphite Nanoplatelet Composites. Nanomaterials 2023, 13, 2518. https://doi.org/10.3390/nano13182518 2079-4991 10.3390/nano13182518 2518 https://www.mdpi.com/2079-4991/13/18/2518 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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