Slow growing behavior in African trypanosomes during adipose tissue colonization

Detalhes bibliográficos
Autor(a) principal: Trindade, Sandra
Data de Publicação: 2022
Outros Autores: De Niz, Mariana, Sequeira, Mariana, Rebelo, Tiago, Bento, Fabio, Dejung, Mario, Narciso, Marta Valido, Escobar, Lara, Ferreira, João, Butter, Falk, Bringaud, Frédéric, Gjini, Erida, Figueiredo, Luisa M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/56325
Resumo: © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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spelling Slow growing behavior in African trypanosomes during adipose tissue colonization© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.When Trypanosoma brucei parasites, the causative agent of sleeping sickness, colonize the adipose tissue, they rewire gene expression. Whether this adaptation affects population behavior and disease treatment remained unknown. By using a mathematical model, we estimate that the population of adipose tissue forms (ATFs) proliferates slower than blood parasites. Analysis of the ATFs proteome, measurement of protein synthesis and proliferation rates confirm that the ATFs divide on average every 12 h, instead of 6 h in the blood. Importantly, the population of ATFs is heterogeneous with parasites doubling times ranging between 5 h and 35 h. Slow-proliferating parasites remain capable of reverting to the fast proliferation profile in blood conditions. Intravital imaging shows that ATFs are refractory to drug treatment. We propose that in adipose tissue, a subpopulation of T. brucei parasites acquire a slow growing behavior, which contributes to disease chronicity and treatment failure.The project leading to this application has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 771714). This work was also supported by IC&DT Programa de Actividades Conjuntas – ref. 016417 “Oneida”, PTDC/BIMMET/4471/2014 and PTDC/CVT-CVT/29161/2017 (FCT). SFRH/BPD/89833/2012 and DL 57/2016/CP1451/CT0019 (FCT) to S.T, LT000047/2019-L (HFSP) and ALTF 1048-2016 (EMBO) to M.D.N, MSCA ITN Cell2Cell fellowship to L.LE and CEECIND/03322/2018 (FCT) to L.M.F. FBr is supported by the Agence National de Recherche (ANR, grant number ANR19-CE15-0004-01: ADIPOTRYP), the Fondation pour le Recherche Médicale (FRM, grant n°EQU201903007845: “Equipe FRM”) and the Laboratoire d’Excellence (grant number ANR-11-LABX-0024:ParaFrap).Springer NatureRepositório da Universidade de LisboaTrindade, SandraDe Niz, MarianaSequeira, MarianaRebelo, TiagoBento, FabioDejung, MarioNarciso, Marta ValidoEscobar, LaraFerreira, JoãoButter, FalkBringaud, FrédéricGjini, EridaFigueiredo, Luisa M.2023-02-15T14:43:49Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/56325engNat Commun. 2022 Dec 8;13(1):754810.1038/s41467-022-34622-w2041-1723info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T18:19:30Zoai:repositorio.ul.pt:10451/56325Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T18:19:30Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Slow growing behavior in African trypanosomes during adipose tissue colonization
title Slow growing behavior in African trypanosomes during adipose tissue colonization
spellingShingle Slow growing behavior in African trypanosomes during adipose tissue colonization
Trindade, Sandra
title_short Slow growing behavior in African trypanosomes during adipose tissue colonization
title_full Slow growing behavior in African trypanosomes during adipose tissue colonization
title_fullStr Slow growing behavior in African trypanosomes during adipose tissue colonization
title_full_unstemmed Slow growing behavior in African trypanosomes during adipose tissue colonization
title_sort Slow growing behavior in African trypanosomes during adipose tissue colonization
author Trindade, Sandra
author_facet Trindade, Sandra
De Niz, Mariana
Sequeira, Mariana
Rebelo, Tiago
Bento, Fabio
Dejung, Mario
Narciso, Marta Valido
Escobar, Lara
Ferreira, João
Butter, Falk
Bringaud, Frédéric
Gjini, Erida
Figueiredo, Luisa M.
author_role author
author2 De Niz, Mariana
Sequeira, Mariana
Rebelo, Tiago
Bento, Fabio
Dejung, Mario
Narciso, Marta Valido
Escobar, Lara
Ferreira, João
Butter, Falk
Bringaud, Frédéric
Gjini, Erida
Figueiredo, Luisa M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Trindade, Sandra
De Niz, Mariana
Sequeira, Mariana
Rebelo, Tiago
Bento, Fabio
Dejung, Mario
Narciso, Marta Valido
Escobar, Lara
Ferreira, João
Butter, Falk
Bringaud, Frédéric
Gjini, Erida
Figueiredo, Luisa M.
description © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01T00:00:00Z
2023-02-15T14:43:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/56325
url http://hdl.handle.net/10451/56325
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nat Commun. 2022 Dec 8;13(1):7548
10.1038/s41467-022-34622-w
2041-1723
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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