Prevalent species in vascular access medical device-associated infections

Detalhes bibliográficos
Autor(a) principal: Machado, Carolina de Matos Santos Coimbra
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/30961
Resumo: Central venous Catheters (CVCs) and Peripheral venous Catheters (PVCs) break the skin barriers, opening a door to microorganisms that potentially cause bloodstream infections (BSIs).This study aimed to infer about the epidemiology of species associated with the use of CVCs and the design of a protocol for the surveillance of PVC tip bacterial colonization at Centro Hospitalar do Baixo Vouga. The rolling of catheter tips was performed using the Maki technique, microorganisms were identified using MALDI-TOF MS and the antimicrobial susceptibility test by disk diffusion method was performed. The majority of patients in this study were men over 60 years old. 39% of the analysed CVC tips tested positive for the presence of microorganisms, of which most were Gram-positive bacteria and only 15% of the total isolated microorganisms were considered the source of a BSI-associated with CVC use. Of the analysed PVC tips, only a small portion had a positive result for the presence of microorganisms, with Staphylococcus epidermidis being the most frequent one. Methicillin-resistant S. epidermidis and S. aureus (MRSE/MRSA) were also observed. The presence of microorganisms even though they belong to the human skin microbiota, in catheter tips is, in itself, a risk for the development of BSIs associated with catheter use.
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spelling Prevalent species in vascular access medical device-associated infectionsVascular access medical devicesCathetersBloodstream infectionsNosocomial infectionsEpidemiologyStaphylococcus sppCentral venous Catheters (CVCs) and Peripheral venous Catheters (PVCs) break the skin barriers, opening a door to microorganisms that potentially cause bloodstream infections (BSIs).This study aimed to infer about the epidemiology of species associated with the use of CVCs and the design of a protocol for the surveillance of PVC tip bacterial colonization at Centro Hospitalar do Baixo Vouga. The rolling of catheter tips was performed using the Maki technique, microorganisms were identified using MALDI-TOF MS and the antimicrobial susceptibility test by disk diffusion method was performed. The majority of patients in this study were men over 60 years old. 39% of the analysed CVC tips tested positive for the presence of microorganisms, of which most were Gram-positive bacteria and only 15% of the total isolated microorganisms were considered the source of a BSI-associated with CVC use. Of the analysed PVC tips, only a small portion had a positive result for the presence of microorganisms, with Staphylococcus epidermidis being the most frequent one. Methicillin-resistant S. epidermidis and S. aureus (MRSE/MRSA) were also observed. The presence of microorganisms even though they belong to the human skin microbiota, in catheter tips is, in itself, a risk for the development of BSIs associated with catheter use.Os cateteres venosos centrais (CVCs) e os cateteres venosos periféricos (CVPs) quebram a barreira da pele, abrindo uma porta a microrganismos potencialmente causadores de infeções da corrente sanguínea (ICS). Este estudo teve como objetivos, inferir sobre a epidemiologia de espécies associadas ao uso de CVCs e o desenho de um protocolo para a vigilância da colonização bacteriana de CVPs no Centro Hospitalar do Baixo Vouga. Foi feito o rolamento de pontas de cateter através da técnica de Maki, identificação de microrganismos através de MALDI-TOF MS e o teste de suscetibilidade a antimicrobianos pelo método de difusão em disco. A maioria dos doentes deste estudo são homens com idade superior a 60 anos. 39% das pontas de CVC analisadas, testaram positivo à presença de microrganismos, dos quais a maioria eram bactérias de Gram-positivo e apenas 15% do total de isolados foram considerados causadores de ICS associada ao uso de CVC. Das pontas de CVP analisadas apenas uma pequena porção teve um resultado positivo para a presença de microrganismos, sendo Staphylococcus epidermidis o microrganismo mais frequente. Observou-se ainda a presença de espécies de S. epidermidis e S. aureus resistentes à meticilina (MRSE/MRSA). A presença de microrganismos, ainda que pertencentes ao microbiota da pele humana, em pontas de cateter é, por si só, um risco para o desenvolvimento de ICS associadas ao uso de cateter.2023-03-10T00:00:00Z2021-02-25T00:00:00Z2021-02-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/30961engMachado, Carolina de Matos Santos Coimbrainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:59:51Zoai:ria.ua.pt:10773/30961Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:02:58.419895Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Prevalent species in vascular access medical device-associated infections
title Prevalent species in vascular access medical device-associated infections
spellingShingle Prevalent species in vascular access medical device-associated infections
Machado, Carolina de Matos Santos Coimbra
Vascular access medical devices
Catheters
Bloodstream infections
Nosocomial infections
Epidemiology
Staphylococcus spp
title_short Prevalent species in vascular access medical device-associated infections
title_full Prevalent species in vascular access medical device-associated infections
title_fullStr Prevalent species in vascular access medical device-associated infections
title_full_unstemmed Prevalent species in vascular access medical device-associated infections
title_sort Prevalent species in vascular access medical device-associated infections
author Machado, Carolina de Matos Santos Coimbra
author_facet Machado, Carolina de Matos Santos Coimbra
author_role author
dc.contributor.author.fl_str_mv Machado, Carolina de Matos Santos Coimbra
dc.subject.por.fl_str_mv Vascular access medical devices
Catheters
Bloodstream infections
Nosocomial infections
Epidemiology
Staphylococcus spp
topic Vascular access medical devices
Catheters
Bloodstream infections
Nosocomial infections
Epidemiology
Staphylococcus spp
description Central venous Catheters (CVCs) and Peripheral venous Catheters (PVCs) break the skin barriers, opening a door to microorganisms that potentially cause bloodstream infections (BSIs).This study aimed to infer about the epidemiology of species associated with the use of CVCs and the design of a protocol for the surveillance of PVC tip bacterial colonization at Centro Hospitalar do Baixo Vouga. The rolling of catheter tips was performed using the Maki technique, microorganisms were identified using MALDI-TOF MS and the antimicrobial susceptibility test by disk diffusion method was performed. The majority of patients in this study were men over 60 years old. 39% of the analysed CVC tips tested positive for the presence of microorganisms, of which most were Gram-positive bacteria and only 15% of the total isolated microorganisms were considered the source of a BSI-associated with CVC use. Of the analysed PVC tips, only a small portion had a positive result for the presence of microorganisms, with Staphylococcus epidermidis being the most frequent one. Methicillin-resistant S. epidermidis and S. aureus (MRSE/MRSA) were also observed. The presence of microorganisms even though they belong to the human skin microbiota, in catheter tips is, in itself, a risk for the development of BSIs associated with catheter use.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-25T00:00:00Z
2021-02-25
2023-03-10T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
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url http://hdl.handle.net/10773/30961
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