Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/50458 |
Resumo: | Background: Exploring different microbial sources for biotechnological production of organic acids is important. Dutch and Thai cow rumen samples were used as inocula to produce organic acid from starch waste in anaerobic reactors. Organic acid production profiles were determined and microbial communities were compared using 16S ribosomal ribonucleic acid gene amplicon pyrosequencing. Results In both reactors, lactate was the main initial product and was associated with growth of Streptococcus spp. (86% average relative abundance). Subsequently, lactate served as a substrate for secondary fermentations. In the reactor inoculated with rumen fluid from the Dutch cow, the relative abundance of Bacillus and Streptococcus increased from the start, and lactate, acetate, formate and ethanol were produced. From day 1.33 to 2, lactate and acetate were degraded, resulting in butyrate production. Butyrate production coincided with a decrease in relative abundance of Streptococcus spp. and increased relative abundances of bacteria of other groups, including Parabacteroides, Sporanaerobacter, Helicobacteraceae, Peptostreptococcaceae and Porphyromonadaceae. In the reactor with the Thai cow inoculum, Streptococcus spp. also increased from the start. When lactate was consumed, acetate, propionate and butyrate were produced (day 34). After day 3, bacteria belonging to five dominant groups, Bacteroides, Pseudoramibacter_Eubacterium, Dysgonomonas, Enterobacteriaceae and Porphyromonadaceae, were detected and these showed significant positive correlations with acetate, propionate and butyrate levels. Conclusions The complexity of rumen microorganisms with high adaptation capacity makes rumen fluid a suitable source to convert organic waste into valuable products without the addition of hydrolytic enzymes. Starch waste is a source for organic acid production, especially lactate. |
id |
RCAP_38ed16039e6fd244d48d337c7d9081be |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/50458 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cowsLactate fermentationMicrobial communitiesRenewable energyRumen fluidOrganic acidsStarch wasteScience & TechnologyBackground: Exploring different microbial sources for biotechnological production of organic acids is important. Dutch and Thai cow rumen samples were used as inocula to produce organic acid from starch waste in anaerobic reactors. Organic acid production profiles were determined and microbial communities were compared using 16S ribosomal ribonucleic acid gene amplicon pyrosequencing. Results In both reactors, lactate was the main initial product and was associated with growth of Streptococcus spp. (86% average relative abundance). Subsequently, lactate served as a substrate for secondary fermentations. In the reactor inoculated with rumen fluid from the Dutch cow, the relative abundance of Bacillus and Streptococcus increased from the start, and lactate, acetate, formate and ethanol were produced. From day 1.33 to 2, lactate and acetate were degraded, resulting in butyrate production. Butyrate production coincided with a decrease in relative abundance of Streptococcus spp. and increased relative abundances of bacteria of other groups, including Parabacteroides, Sporanaerobacter, Helicobacteraceae, Peptostreptococcaceae and Porphyromonadaceae. In the reactor with the Thai cow inoculum, Streptococcus spp. also increased from the start. When lactate was consumed, acetate, propionate and butyrate were produced (day 34). After day 3, bacteria belonging to five dominant groups, Bacteroides, Pseudoramibacter_Eubacterium, Dysgonomonas, Enterobacteriaceae and Porphyromonadaceae, were detected and these showed significant positive correlations with acetate, propionate and butyrate levels. Conclusions The complexity of rumen microorganisms with high adaptation capacity makes rumen fluid a suitable source to convert organic waste into valuable products without the addition of hydrolytic enzymes. Starch waste is a source for organic acid production, especially lactate.Susakul Palakawong Na Ayudthaya was financially supported by a Royal Thai Government Scholarship, Thailand. We thank Stang Pumisutapon at The Charoen Pokphand Group, Thailand and Rik Verkerk at the Department of Animal Sciences, Wageningen University for providing rumen fluids, Detmer Sipkema for his help with pyrosequencing analysis and Gerben Hermes for his support using the CANOCO program and Bart Nijsse for technical support. Research of Alfons J. M. Stams is supported by ERC Grant Project 323009. Alfons J. M. Stams and Willem M. de Vos are supported by Gravitation Grant Project 024.002.002 from the Netherlands Ministry of Education, Culture and Science.info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoPalakawong Na Ayudthaya, Susakulvan de Weijer, Antonius H. P.van Gelder, Antonie H.Stams, Alfons Johannes Mariade Vos, Willem M.Plugge, Caroline M.2018-01-252018-01-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/50458engPalakawong Na Ayudthaya, Susakul; van de Weijer, Antonius H. P.; van Gelder, Antonie H.; Stams, A. J. M.; de Vos, Willem M.; Plugge, Caroline M., Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows. Biotechnology for Biofuels, 11(13), 20181754-683410.1186/s13068-018-1012-4http://www.biotechnologyforbiofuels.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:23Zoai:repositorium.sdum.uminho.pt:1822/50458Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:38.766840Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows |
title |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows |
spellingShingle |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows Palakawong Na Ayudthaya, Susakul Lactate fermentation Microbial communities Renewable energy Rumen fluid Organic acids Starch waste Science & Technology |
title_short |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows |
title_full |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows |
title_fullStr |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows |
title_full_unstemmed |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows |
title_sort |
Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows |
author |
Palakawong Na Ayudthaya, Susakul |
author_facet |
Palakawong Na Ayudthaya, Susakul van de Weijer, Antonius H. P. van Gelder, Antonie H. Stams, Alfons Johannes Maria de Vos, Willem M. Plugge, Caroline M. |
author_role |
author |
author2 |
van de Weijer, Antonius H. P. van Gelder, Antonie H. Stams, Alfons Johannes Maria de Vos, Willem M. Plugge, Caroline M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Palakawong Na Ayudthaya, Susakul van de Weijer, Antonius H. P. van Gelder, Antonie H. Stams, Alfons Johannes Maria de Vos, Willem M. Plugge, Caroline M. |
dc.subject.por.fl_str_mv |
Lactate fermentation Microbial communities Renewable energy Rumen fluid Organic acids Starch waste Science & Technology |
topic |
Lactate fermentation Microbial communities Renewable energy Rumen fluid Organic acids Starch waste Science & Technology |
description |
Background: Exploring different microbial sources for biotechnological production of organic acids is important. Dutch and Thai cow rumen samples were used as inocula to produce organic acid from starch waste in anaerobic reactors. Organic acid production profiles were determined and microbial communities were compared using 16S ribosomal ribonucleic acid gene amplicon pyrosequencing. Results In both reactors, lactate was the main initial product and was associated with growth of Streptococcus spp. (86% average relative abundance). Subsequently, lactate served as a substrate for secondary fermentations. In the reactor inoculated with rumen fluid from the Dutch cow, the relative abundance of Bacillus and Streptococcus increased from the start, and lactate, acetate, formate and ethanol were produced. From day 1.33 to 2, lactate and acetate were degraded, resulting in butyrate production. Butyrate production coincided with a decrease in relative abundance of Streptococcus spp. and increased relative abundances of bacteria of other groups, including Parabacteroides, Sporanaerobacter, Helicobacteraceae, Peptostreptococcaceae and Porphyromonadaceae. In the reactor with the Thai cow inoculum, Streptococcus spp. also increased from the start. When lactate was consumed, acetate, propionate and butyrate were produced (day 34). After day 3, bacteria belonging to five dominant groups, Bacteroides, Pseudoramibacter_Eubacterium, Dysgonomonas, Enterobacteriaceae and Porphyromonadaceae, were detected and these showed significant positive correlations with acetate, propionate and butyrate levels. Conclusions The complexity of rumen microorganisms with high adaptation capacity makes rumen fluid a suitable source to convert organic waste into valuable products without the addition of hydrolytic enzymes. Starch waste is a source for organic acid production, especially lactate. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-25 2018-01-25T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/50458 |
url |
http://hdl.handle.net/1822/50458 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Palakawong Na Ayudthaya, Susakul; van de Weijer, Antonius H. P.; van Gelder, Antonie H.; Stams, A. J. M.; de Vos, Willem M.; Plugge, Caroline M., Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows. Biotechnology for Biofuels, 11(13), 2018 1754-6834 10.1186/s13068-018-1012-4 http://www.biotechnologyforbiofuels.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133036008177664 |