Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer

Detalhes bibliográficos
Autor(a) principal: Hall, Michael J
Data de Publicação: 2022
Outros Autores: Lopes-Ventura, Sara, Neto, Matilde V, Charneca, João, Zoio, Patricia, Seabra, Miguel C, Oliva, Abel, Barral, Duarte C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/137368
Resumo: Funding: We thank the Electron Microscopy Facility at the Instituto Gulbenkian de Ciência, the Pathological Anatomy service in the Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG) and the Histology Facility at CEDOC for technical assistance. This project was supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal through FCT Unit iNOVA4Health – UIDB/04462/2020 and UIDP/04462/2020, a programme financially supported by FCT / Ministério da Ciência, Tecnologia e Ensino Superior, through national funds, grant PTDC/BIA-CEL/29765/2017, PhD fellowships 2020.08528.BD (SLV), PD/BD/128164/2016, PD/BD/137442/2018 (MVN) and PD/BD/136905/2018 (JC) and the FCT Investigator Program to DCB (IF/00501/2014/CP1252/CT0001).
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spelling Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transferSDG 3 - Good Health and Well-beingFunding: We thank the Electron Microscopy Facility at the Instituto Gulbenkian de Ciência, the Pathological Anatomy service in the Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG) and the Histology Facility at CEDOC for technical assistance. This project was supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal through FCT Unit iNOVA4Health – UIDB/04462/2020 and UIDP/04462/2020, a programme financially supported by FCT / Ministério da Ciência, Tecnologia e Ensino Superior, through national funds, grant PTDC/BIA-CEL/29765/2017, PhD fellowships 2020.08528.BD (SLV), PD/BD/128164/2016, PD/BD/137442/2018 (MVN) and PD/BD/136905/2018 (JC) and the FCT Investigator Program to DCB (IF/00501/2014/CP1252/CT0001).The skin acts as a barrier to environmental insults and provides many vital functions. One of these is to shield DNA from harmful UV radiation, which is achieved by skin pigmentation arising as melanin is produced and dispersed within the epidermal layer. This is a crucial defence against DNA damage, photo-ageing and skin cancer. The mechanisms and regulation of melanogenesis and melanin transfer involve extensive crosstalk between melanocytes and keratinocytes in the epidermis, as well as fibroblasts in the dermal layer. Although the predominant mechanism of melanin transfer continues to be debated and several plausible models have been proposed, we and others previously provided evidence for a coupled exo/phagocytosis model. Herein, we performed histology and immunohistochemistry analyses and demonstrated that a newly developed full-thickness 3D reconstructed human pigmented skin model and an epidermis-only model exhibit dispersed pigment throughout keratinocytes in the epidermis. Transmission electron microscopy revealed melanocores between melanocytes and keratinocytes, suggesting that melanin is transferred through coupled exocytosis/phagocytosis of the melanosome core, or melanocore, similar to our previous observations in human skin biopsies. We therefore present evidence that our in vitro models of pigmented human skin show epidermal pigmentation comparable to human skin. These findings have a high value for studies of skin pigmentation mechanisms and pigmentary disorders, whilst reducing the reliance on animal models and human skin biopsies.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNHall, Michael JLopes-Ventura, SaraNeto, Matilde VCharneca, JoãoZoio, PatriciaSeabra, Miguel COliva, AbelBarral, Duarte C2022-05-03T22:48:34Z2022-072022-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/137368eng1755-1471PURE: 42836300https://doi.org/10.1111/pcmr.13039info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:14:54Zoai:run.unl.pt:10362/137368Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:49.756413Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer
title Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer
spellingShingle Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer
Hall, Michael J
SDG 3 - Good Health and Well-being
title_short Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer
title_full Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer
title_fullStr Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer
title_full_unstemmed Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer
title_sort Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer
author Hall, Michael J
author_facet Hall, Michael J
Lopes-Ventura, Sara
Neto, Matilde V
Charneca, João
Zoio, Patricia
Seabra, Miguel C
Oliva, Abel
Barral, Duarte C
author_role author
author2 Lopes-Ventura, Sara
Neto, Matilde V
Charneca, João
Zoio, Patricia
Seabra, Miguel C
Oliva, Abel
Barral, Duarte C
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Hall, Michael J
Lopes-Ventura, Sara
Neto, Matilde V
Charneca, João
Zoio, Patricia
Seabra, Miguel C
Oliva, Abel
Barral, Duarte C
dc.subject.por.fl_str_mv SDG 3 - Good Health and Well-being
topic SDG 3 - Good Health and Well-being
description Funding: We thank the Electron Microscopy Facility at the Instituto Gulbenkian de Ciência, the Pathological Anatomy service in the Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG) and the Histology Facility at CEDOC for technical assistance. This project was supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal through FCT Unit iNOVA4Health – UIDB/04462/2020 and UIDP/04462/2020, a programme financially supported by FCT / Ministério da Ciência, Tecnologia e Ensino Superior, through national funds, grant PTDC/BIA-CEL/29765/2017, PhD fellowships 2020.08528.BD (SLV), PD/BD/128164/2016, PD/BD/137442/2018 (MVN) and PD/BD/136905/2018 (JC) and the FCT Investigator Program to DCB (IF/00501/2014/CP1252/CT0001).
publishDate 2022
dc.date.none.fl_str_mv 2022-05-03T22:48:34Z
2022-07
2022-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/137368
url http://hdl.handle.net/10362/137368
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1755-1471
PURE: 42836300
https://doi.org/10.1111/pcmr.13039
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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