Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/76693 |
Resumo: | Antimicrobial resistance is among the biggest threats to mankind today. There are two alternative antimicrobial therapies that may help to control multidrug-resistant bacteria. In phage therapy, natural antagonists of bacteria, lytic phages, are harnessed to fight pathogens. In antimicrobial photodynamic therapy (aPDT), a photosensitizer, molecular oxygen, and light are used to produce reactive oxygen species (ROS) that inflict lethal damage on pathogens. Since aPDT destroys multiple essential components in targeted pathogens, aPDT resistance is unlikely. However, the challenge in aPDT is to maximize target specificity and minimize collateral oxidative damage to host cells. We now present an antimicrobial approach that combines the best features of both alternative therapies, namely, the high target specificity of phages and the efficacy of aPDT. This is achieved by conjugating the specific cell-binding domain from a phage protein to a near-infrared photosensitizer. aPDT with the resulting conjugate shows high target specificity toward MRSA with minimal side effects. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domainCell-binding domainEndolysinAntimicrobial photodynamic therapyIntracellular Staphylococcus aureusStaphylococcus epidermidisStaphylococcus aureusBiofilmsIntracellular bacteriaAntimicrobial resistance is among the biggest threats to mankind today. There are two alternative antimicrobial therapies that may help to control multidrug-resistant bacteria. In phage therapy, natural antagonists of bacteria, lytic phages, are harnessed to fight pathogens. In antimicrobial photodynamic therapy (aPDT), a photosensitizer, molecular oxygen, and light are used to produce reactive oxygen species (ROS) that inflict lethal damage on pathogens. Since aPDT destroys multiple essential components in targeted pathogens, aPDT resistance is unlikely. However, the challenge in aPDT is to maximize target specificity and minimize collateral oxidative damage to host cells. We now present an antimicrobial approach that combines the best features of both alternative therapies, namely, the high target specificity of phages and the efficacy of aPDT. This is achieved by conjugating the specific cell-binding domain from a phage protein to a near-infrared photosensitizer. aPDT with the resulting conjugate shows high target specificity toward MRSA with minimal side effects.FCT -ZonMw(713660)info:eu-repo/semantics/publishedVersionAmerican Society for Microbiology (ASM)Universidade do MinhoBispo, MafaldaSantos, Sílvio Roberto BrancoMelo, Luís Daniel RodriguesAzeredo, JoanaDijl, Jan Maarten van2022-03-232022-03-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/76693engBispo, Mafalda; Santos, Sílvio Roberto Branco; Melo, Luís Daniel Rodrigues; Azeredo, Joana; Dijl, Jan Maarten van; Rosato, Adriana E., Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain. Microbiology Spectrum, 10(1), e01466-21, 20222165-049710.1128/spectrum.01466-2135196798https://journals.asm.org/journal/spectruminfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:54:03Zoai:repositorium.sdum.uminho.pt:1822/76693Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:34.101823Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain |
title |
Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain |
spellingShingle |
Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain Bispo, Mafalda Cell-binding domain Endolysin Antimicrobial photodynamic therapy Intracellular Staphylococcus aureus Staphylococcus epidermidis Staphylococcus aureus Biofilms Intracellular bacteria |
title_short |
Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain |
title_full |
Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain |
title_fullStr |
Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain |
title_full_unstemmed |
Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain |
title_sort |
Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain |
author |
Bispo, Mafalda |
author_facet |
Bispo, Mafalda Santos, Sílvio Roberto Branco Melo, Luís Daniel Rodrigues Azeredo, Joana Dijl, Jan Maarten van |
author_role |
author |
author2 |
Santos, Sílvio Roberto Branco Melo, Luís Daniel Rodrigues Azeredo, Joana Dijl, Jan Maarten van |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Bispo, Mafalda Santos, Sílvio Roberto Branco Melo, Luís Daniel Rodrigues Azeredo, Joana Dijl, Jan Maarten van |
dc.subject.por.fl_str_mv |
Cell-binding domain Endolysin Antimicrobial photodynamic therapy Intracellular Staphylococcus aureus Staphylococcus epidermidis Staphylococcus aureus Biofilms Intracellular bacteria |
topic |
Cell-binding domain Endolysin Antimicrobial photodynamic therapy Intracellular Staphylococcus aureus Staphylococcus epidermidis Staphylococcus aureus Biofilms Intracellular bacteria |
description |
Antimicrobial resistance is among the biggest threats to mankind today. There are two alternative antimicrobial therapies that may help to control multidrug-resistant bacteria. In phage therapy, natural antagonists of bacteria, lytic phages, are harnessed to fight pathogens. In antimicrobial photodynamic therapy (aPDT), a photosensitizer, molecular oxygen, and light are used to produce reactive oxygen species (ROS) that inflict lethal damage on pathogens. Since aPDT destroys multiple essential components in targeted pathogens, aPDT resistance is unlikely. However, the challenge in aPDT is to maximize target specificity and minimize collateral oxidative damage to host cells. We now present an antimicrobial approach that combines the best features of both alternative therapies, namely, the high target specificity of phages and the efficacy of aPDT. This is achieved by conjugating the specific cell-binding domain from a phage protein to a near-infrared photosensitizer. aPDT with the resulting conjugate shows high target specificity toward MRSA with minimal side effects. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-23 2022-03-23T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/76693 |
url |
http://hdl.handle.net/1822/76693 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bispo, Mafalda; Santos, Sílvio Roberto Branco; Melo, Luís Daniel Rodrigues; Azeredo, Joana; Dijl, Jan Maarten van; Rosato, Adriana E., Targeted antimicrobial photodynamic therapy of biofilm-embedded and intracellular staphylococci with a phage endolysins cell binding domain. Microbiology Spectrum, 10(1), e01466-21, 2022 2165-0497 10.1128/spectrum.01466-21 35196798 https://journals.asm.org/journal/spectrum |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology (ASM) |
publisher.none.fl_str_mv |
American Society for Microbiology (ASM) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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_version_ |
1799133132701564928 |