Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms

Detalhes bibliográficos
Autor(a) principal: Enea, Maria
Data de Publicação: 2022
Outros Autores: Peixoto de Almeida, Miguel, Dias, André, Ferreira, Beatriz, Bernardes, Carlos, Flores, Orfeu, Pereira, Eulália, Franco, Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/150483
Resumo: Funding Information: This work was financed by national funds from FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the following Projects: LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB; PTDC/NAN‐MAT/30589/2017 (NANOMODE). Dr. Rocío Jurado is acknowledged for initial work under the scope of the NANOMODE Project. Publisher Copyright: © 2022 The Authors. Particle & Particle Systems Characterization published by Wiley-VCH GmbH.
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spelling Improved Gold Nanoprobes for Detection of Single Nucleotide PolymorphismsThe Influence of SizeDNA detectiongold nanoparticlesgold nanoprobeslactose intolerancemolecular detectionnon-cross-linking methodsingle nucleotide polymorphismChemistry(all)Materials Science(all)Condensed Matter PhysicsFunding Information: This work was financed by national funds from FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the following Projects: LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB; PTDC/NAN‐MAT/30589/2017 (NANOMODE). Dr. Rocío Jurado is acknowledged for initial work under the scope of the NANOMODE Project. Publisher Copyright: © 2022 The Authors. Particle & Particle Systems Characterization published by Wiley-VCH GmbH.Gold nanoparticles (AuNPs) are widely used in biomedical diagnostics due to their unique plasmonic properties, with larger AuNPs showing higher extinction coefficients for the plasmon band and, consequently, more intense colors than the more commonly used spherical 20 nm AuNPs. Other factors can be important in the performance of different-sized AuNPs, including surface area, colloidal stability, and curvature effects. Here, the properties of spherical 20 nm AuNPs and 35 nm AuNPs functionalized with a specific thiol-modified oligonucleotide–Au nanoprobes are compared when used on a colorimetric assay for the detection of a single nucleotide polymorphism related to lactose intolerance in humans. Successful functionalization of AuNPs is assessed by UV–vis spectroscopy, agarose gel electrophoresis, dynamic and electrophoretic light scattering, and nanoparticle tracking analysis. Statistical differences between Au-nanoprobe DNA target groups are calculated using analysis of variance and a post hoc Tukey's test. These results show that both 35 and 20 nm Au nanoprobes have similar detection limits using a 0.15 nmol dm−3 nanoprobe concentration compared to 2.5 nmol dm−3. Interestingly, the use of 35 nm Au nanoprobes allows a reduction of 80% and 48% in the amount of gold and oligonucleotide used in the assay, respectively.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNEnea, MariaPeixoto de Almeida, MiguelDias, AndréFerreira, BeatrizBernardes, CarlosFlores, OrfeuPereira, EuláliaFranco, Ricardo2023-03-13T22:26:39Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/150483eng0934-0866PURE: 55545855https://doi.org/10.1002/ppsc.202200137info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:32:27Zoai:run.unl.pt:10362/150483Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:08.508245Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms
The Influence of Size
title Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms
spellingShingle Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms
Enea, Maria
DNA detection
gold nanoparticles
gold nanoprobes
lactose intolerance
molecular detection
non-cross-linking method
single nucleotide polymorphism
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
title_short Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms
title_full Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms
title_fullStr Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms
title_full_unstemmed Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms
title_sort Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms
author Enea, Maria
author_facet Enea, Maria
Peixoto de Almeida, Miguel
Dias, André
Ferreira, Beatriz
Bernardes, Carlos
Flores, Orfeu
Pereira, Eulália
Franco, Ricardo
author_role author
author2 Peixoto de Almeida, Miguel
Dias, André
Ferreira, Beatriz
Bernardes, Carlos
Flores, Orfeu
Pereira, Eulália
Franco, Ricardo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Enea, Maria
Peixoto de Almeida, Miguel
Dias, André
Ferreira, Beatriz
Bernardes, Carlos
Flores, Orfeu
Pereira, Eulália
Franco, Ricardo
dc.subject.por.fl_str_mv DNA detection
gold nanoparticles
gold nanoprobes
lactose intolerance
molecular detection
non-cross-linking method
single nucleotide polymorphism
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
topic DNA detection
gold nanoparticles
gold nanoprobes
lactose intolerance
molecular detection
non-cross-linking method
single nucleotide polymorphism
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
description Funding Information: This work was financed by national funds from FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the following Projects: LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB; PTDC/NAN‐MAT/30589/2017 (NANOMODE). Dr. Rocío Jurado is acknowledged for initial work under the scope of the NANOMODE Project. Publisher Copyright: © 2022 The Authors. Particle & Particle Systems Characterization published by Wiley-VCH GmbH.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2022-12-01T00:00:00Z
2023-03-13T22:26:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/150483
url http://hdl.handle.net/10362/150483
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0934-0866
PURE: 55545855
https://doi.org/10.1002/ppsc.202200137
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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