Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/83075 |
Resumo: | More than 50% of all prostate cancer (PCa) patients are treated by radiotherapy (RT). Radioresistance and cancer recurrence are two consequences of the therapy and are related to dose heterogeneity and non-selectivity between normal and tumoral cells. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome these therapeutic limitations of RT. This study assessed the biological interaction of different morphologies of AuNPs with ionizing radiation (IR) in PCa cells. To achieve that aim, three different amine-pegylated AuNPs were synthesized with distinct sizes and shapes (spherical, AuNPsp-PEG, star, AuNPst-PEG, and rods, AuNPr-PEG) and viability, injury and colony assays were used to analyze their biological effect on PCa cells (PC3, DU145, and LNCaP) when submitted to the accumulative fraction of RT. The combinatory effect of AuNPs with IR decreased cell viability and increased apoptosis compared to cells treated only with IR or untreated cells. Additionally, our results showed an increase in the sensitization enhancement ratio by cells treated with AuNPs and IR, and this effect is cell line dependent. Our findings support that the design of AuNPs modulated their cellular behavior and suggested that AuNPs could improve the RT efficacy in PCa cells. |
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Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell linesGold nanoparticlesRadiotherapyRadiosensitizing effectProstate cancerIn vitro assayScience & TechnologyMore than 50% of all prostate cancer (PCa) patients are treated by radiotherapy (RT). Radioresistance and cancer recurrence are two consequences of the therapy and are related to dose heterogeneity and non-selectivity between normal and tumoral cells. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome these therapeutic limitations of RT. This study assessed the biological interaction of different morphologies of AuNPs with ionizing radiation (IR) in PCa cells. To achieve that aim, three different amine-pegylated AuNPs were synthesized with distinct sizes and shapes (spherical, AuNPsp-PEG, star, AuNPst-PEG, and rods, AuNPr-PEG) and viability, injury and colony assays were used to analyze their biological effect on PCa cells (PC3, DU145, and LNCaP) when submitted to the accumulative fraction of RT. The combinatory effect of AuNPs with IR decreased cell viability and increased apoptosis compared to cells treated only with IR or untreated cells. Additionally, our results showed an increase in the sensitization enhancement ratio by cells treated with AuNPs and IR, and this effect is cell line dependent. Our findings support that the design of AuNPs modulated their cellular behavior and suggested that AuNPs could improve the RT efficacy in PCa cells.The author is grateful for the financial support of Foundation for Science and Technology (grant reference: SFRH/BD/138271/2018).info:eu-repo/semantics/publishedVersionMultidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoSoares, SílviaFaria, IsabelAires, FátimaMonteiro, ArmandaPinto, GabrielaSales, Maria GoretiCorrea-Duarte, Miguel A.Guerreiro, Susana G.Fernandes, Rúben2023-02-132023-02-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/83075engSoares, Sílvia; Faria, Isabel; Aires, Fátima; Monteiro, Armanda; Pinto, Gabriela; Sales, M. G. F.; Correa-Duarte, Miguel A.; Guerreiro, Susana G.; Fernandes, Rúben, Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines. International Journal of Molecular Sciences, 24(4), 4122, 20231424-67831661-65961422-006710.3390/ijms24044122368355384122http://www.mdpi.com/journal/ijmsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:39Zoai:repositorium.sdum.uminho.pt:1822/83075Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:50.306617Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines |
title |
Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines |
spellingShingle |
Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines Soares, Sílvia Gold nanoparticles Radiotherapy Radiosensitizing effect Prostate cancer In vitro assay Science & Technology |
title_short |
Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines |
title_full |
Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines |
title_fullStr |
Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines |
title_full_unstemmed |
Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines |
title_sort |
Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines |
author |
Soares, Sílvia |
author_facet |
Soares, Sílvia Faria, Isabel Aires, Fátima Monteiro, Armanda Pinto, Gabriela Sales, Maria Goreti Correa-Duarte, Miguel A. Guerreiro, Susana G. Fernandes, Rúben |
author_role |
author |
author2 |
Faria, Isabel Aires, Fátima Monteiro, Armanda Pinto, Gabriela Sales, Maria Goreti Correa-Duarte, Miguel A. Guerreiro, Susana G. Fernandes, Rúben |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Soares, Sílvia Faria, Isabel Aires, Fátima Monteiro, Armanda Pinto, Gabriela Sales, Maria Goreti Correa-Duarte, Miguel A. Guerreiro, Susana G. Fernandes, Rúben |
dc.subject.por.fl_str_mv |
Gold nanoparticles Radiotherapy Radiosensitizing effect Prostate cancer In vitro assay Science & Technology |
topic |
Gold nanoparticles Radiotherapy Radiosensitizing effect Prostate cancer In vitro assay Science & Technology |
description |
More than 50% of all prostate cancer (PCa) patients are treated by radiotherapy (RT). Radioresistance and cancer recurrence are two consequences of the therapy and are related to dose heterogeneity and non-selectivity between normal and tumoral cells. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome these therapeutic limitations of RT. This study assessed the biological interaction of different morphologies of AuNPs with ionizing radiation (IR) in PCa cells. To achieve that aim, three different amine-pegylated AuNPs were synthesized with distinct sizes and shapes (spherical, AuNPsp-PEG, star, AuNPst-PEG, and rods, AuNPr-PEG) and viability, injury and colony assays were used to analyze their biological effect on PCa cells (PC3, DU145, and LNCaP) when submitted to the accumulative fraction of RT. The combinatory effect of AuNPs with IR decreased cell viability and increased apoptosis compared to cells treated only with IR or untreated cells. Additionally, our results showed an increase in the sensitization enhancement ratio by cells treated with AuNPs and IR, and this effect is cell line dependent. Our findings support that the design of AuNPs modulated their cellular behavior and suggested that AuNPs could improve the RT efficacy in PCa cells. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-02-13 2023-02-13T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/83075 |
url |
https://hdl.handle.net/1822/83075 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Soares, Sílvia; Faria, Isabel; Aires, Fátima; Monteiro, Armanda; Pinto, Gabriela; Sales, M. G. F.; Correa-Duarte, Miguel A.; Guerreiro, Susana G.; Fernandes, Rúben, Application of gold nanoparticles as radiosensitizer for metastatic prostate cancer cell lines. International Journal of Molecular Sciences, 24(4), 4122, 2023 1424-6783 1661-6596 1422-0067 10.3390/ijms24044122 36835538 4122 http://www.mdpi.com/journal/ijms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132578344599552 |