Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1

Detalhes bibliográficos
Autor(a) principal: Severino, Paulo F
Data de Publicação: 2018
Outros Autores: Silva, Mariana, Carrascal, Mylene, Malagolini, Nadia, Chiricolo, Mariella, Venturi, Giulia, Barbaro Forleo, Roberto, Astolfi, Annalisa, Catera, Mariangela, Videira, Paula A, Dall'Olio, Fabio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/31462
Resumo: This work was supported by: Portuguese Foundation for Science and Technology (FCT) PhD grants SFRH/BD/45120/2008 (Paulo F. Severino), SFRH/BD/81860/2011 (Mariana Silva) and SFRH/BD/100970/2014 (Mylene Carrascal), Liga Portuguesa Contra o Cancro 2011 (Mylene A. Carrascal); Premio Bluepharma Inovacao Universidade de Coimbra and the Santander Totta/Universidade NOVA de Lisboa prizes (Paula Videira). Grants from the University of Bologna (Fabio Dall'Olio). Mariangela Catera and Giulia Venturi are PhD students supported by grants from the University of Bologna.
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spelling Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1Bacillus Calmette-GuérinGlycosylationSialyl T antigenSialyltransferasThomsen-Friedenreich antigeSDG 3 - Good Health and Well-beingThis work was supported by: Portuguese Foundation for Science and Technology (FCT) PhD grants SFRH/BD/45120/2008 (Paulo F. Severino), SFRH/BD/81860/2011 (Mariana Silva) and SFRH/BD/100970/2014 (Mylene Carrascal), Liga Portuguesa Contra o Cancro 2011 (Mylene A. Carrascal); Premio Bluepharma Inovacao Universidade de Coimbra and the Santander Totta/Universidade NOVA de Lisboa prizes (Paula Videira). Grants from the University of Bologna (Fabio Dall'Olio). Mariangela Catera and Giulia Venturi are PhD students supported by grants from the University of Bologna.BACKGROUND: Treatment with Bacillus Calmette-Guérin (BCG) is the gold standard adjuvant immunotherapy of non-muscle invasive bladder cancer (NMIBC), although it fails in one third of the patients. NMIBC expresses two tumor-associated O-linked carbohydrates: the disaccharide (Galβ1,3GalNAc) Thomsen-Friedenreich (T) antigen, and its sialylated counterpart (Siaα2,3Galβ1,3GalNAc) sialyl-T (sT), synthesized by sialyltransferase ST3GAL1, whose roles in BCG response are unknown. METHODS: The human bladder cancer (BC) cell line HT1376 strongly expressing the T antigen, was retrovirally transduced with the ST3GAL1 cDNA or with an empty vector, yielding the cell lines HT1376sTand HT1376T, that express, respectively, either the sT or the T antigens. Cells were in vitro challenged with BCG. Whole gene expression was studied by microarray technology, cytokine secretion was measured by multiplex immune-beads assay. Human macrophages derived from blood monocytes were challenged with the secretome of BCG-challenged BC cells. RESULTS: The secretome from BCG-challenged HT1376sTcells induced a stronger macrophage secretion of IL-6, IL-1β, TNFα and IL-10 than that of HT1376Tcells. Transcriptomic analysis revealed that ST3GAL1 overexpression and T/sT replacement modulated hundreds of genes. Several genes preserving genomic stability were down-regulated in HT1376sTcells which, as a consequence, displayed increased sensitivity to oxidative damage. After BCG challenge, the transcriptome of HT1376sTcells showed higher susceptibility to BCG modulation than that of HT1376Tcells. CONCLUSIONS: High ST3GAL1 expression and T/sT replacement in BCG challenged-BC cancer cells induce a stronger macrophage response and alter the gene expression towards genomic instability, indicating a potential impact on BC biology and patient's response to BCG.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNSeverino, Paulo FSilva, MarianaCarrascal, MyleneMalagolini, NadiaChiricolo, MariellaVenturi, GiuliaBarbaro Forleo, RobertoAstolfi, AnnalisaCatera, MariangelaVideira, Paula ADall'Olio, Fabio2018-02-28T23:21:31Z2018-02-172018-02-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/31462eng1471-2407PURE: 3639788https://doi.org/10.1186/s12885-018-4107-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:17:25Zoai:run.unl.pt:10362/31462Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:29:40.225985Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1
title Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1
spellingShingle Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1
Severino, Paulo F
Bacillus Calmette-Guérin
Glycosylation
Sialyl T antigen
Sialyltransferas
Thomsen-Friedenreich antige
SDG 3 - Good Health and Well-being
title_short Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1
title_full Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1
title_fullStr Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1
title_full_unstemmed Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1
title_sort Oxidative damage and response to Bacillus Calmette-Guérin in bladder cancer cells expressing sialyltransferase ST3GAL1
author Severino, Paulo F
author_facet Severino, Paulo F
Silva, Mariana
Carrascal, Mylene
Malagolini, Nadia
Chiricolo, Mariella
Venturi, Giulia
Barbaro Forleo, Roberto
Astolfi, Annalisa
Catera, Mariangela
Videira, Paula A
Dall'Olio, Fabio
author_role author
author2 Silva, Mariana
Carrascal, Mylene
Malagolini, Nadia
Chiricolo, Mariella
Venturi, Giulia
Barbaro Forleo, Roberto
Astolfi, Annalisa
Catera, Mariangela
Videira, Paula A
Dall'Olio, Fabio
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Severino, Paulo F
Silva, Mariana
Carrascal, Mylene
Malagolini, Nadia
Chiricolo, Mariella
Venturi, Giulia
Barbaro Forleo, Roberto
Astolfi, Annalisa
Catera, Mariangela
Videira, Paula A
Dall'Olio, Fabio
dc.subject.por.fl_str_mv Bacillus Calmette-Guérin
Glycosylation
Sialyl T antigen
Sialyltransferas
Thomsen-Friedenreich antige
SDG 3 - Good Health and Well-being
topic Bacillus Calmette-Guérin
Glycosylation
Sialyl T antigen
Sialyltransferas
Thomsen-Friedenreich antige
SDG 3 - Good Health and Well-being
description This work was supported by: Portuguese Foundation for Science and Technology (FCT) PhD grants SFRH/BD/45120/2008 (Paulo F. Severino), SFRH/BD/81860/2011 (Mariana Silva) and SFRH/BD/100970/2014 (Mylene Carrascal), Liga Portuguesa Contra o Cancro 2011 (Mylene A. Carrascal); Premio Bluepharma Inovacao Universidade de Coimbra and the Santander Totta/Universidade NOVA de Lisboa prizes (Paula Videira). Grants from the University of Bologna (Fabio Dall'Olio). Mariangela Catera and Giulia Venturi are PhD students supported by grants from the University of Bologna.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-28T23:21:31Z
2018-02-17
2018-02-17T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/31462
url http://hdl.handle.net/10362/31462
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1471-2407
PURE: 3639788
https://doi.org/10.1186/s12885-018-4107-1
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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