Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/12446 |
Resumo: | Although microplastics (MPs) are distributed globally in the marine environment, a great deal of unknowns relating to their ecotoxicological effects on the marine biota remains. Due to their lipophilic nature, microplastics have the potential to adsorb persistent organic pollutants present in contaminated regions, which may increase their detrimental impact once assimilated by organisms. This study investigates the ecotoxicological effects of exposure to low-density polyethylene (LDPE) microplastics (11-13 beta m), with and without adsorbed contaminants (benzo[a]pyrene- BaP and perfluorooctane sulfonic acid-PFOS), in the peppery furrow shell clam, Scrobicularia plana. Environmentally relevant concentrations of contaminants (BaP-16.87 +/- 0.22 mu g g(-1) and PFOS-70.22 +/- 12.41 mu g g(-1)) were adsorbed to microplastics to evaluate the potential role of plastic particles as a source of chemical contamination once ingested. S. plana were exposed to microplastics, at a concentration of 1 mg L-1, in a water-sediment exposure setup for 14 days. Clams were sampled at the beginning of the experiment (day 0) and after 3, 7, and 14 days. BaP accumulation, in whole clam tissues, was analyzed. A multi-biomarker assessment was conducted in the gills, digestive gland, and haemolymph of clams to clarify the effects of exposure. This included the quantification of antioxidant (superoxide dismutase, catalase, glutathione peroxidase) and biotransformation (glutathione-Stransferases) enzyme activities, oxidative damage (lipid peroxidation levels), genotoxicity (single and double strand DNA breaks), and neurotoxicity (acetylcholinesterase activity). Results suggest a potential mechanical injury of gills caused by ingestion of microplastics that may also affect the analyzed biomarkers. The digestive gland seems less affected by mechanical damage caused by virgin microplastic exposure, with the MPs-adsorbed BaP and PFOS exerting a negative influence over the assessed biomarkers in this tissue. |
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Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia planaPolyethyleneBenzo[a]pyrenePerfluorooctane sulfonic acidDNA damageOxidative stressNeurotoxicityOxidative damageAlthough microplastics (MPs) are distributed globally in the marine environment, a great deal of unknowns relating to their ecotoxicological effects on the marine biota remains. Due to their lipophilic nature, microplastics have the potential to adsorb persistent organic pollutants present in contaminated regions, which may increase their detrimental impact once assimilated by organisms. This study investigates the ecotoxicological effects of exposure to low-density polyethylene (LDPE) microplastics (11-13 beta m), with and without adsorbed contaminants (benzo[a]pyrene- BaP and perfluorooctane sulfonic acid-PFOS), in the peppery furrow shell clam, Scrobicularia plana. Environmentally relevant concentrations of contaminants (BaP-16.87 +/- 0.22 mu g g(-1) and PFOS-70.22 +/- 12.41 mu g g(-1)) were adsorbed to microplastics to evaluate the potential role of plastic particles as a source of chemical contamination once ingested. S. plana were exposed to microplastics, at a concentration of 1 mg L-1, in a water-sediment exposure setup for 14 days. Clams were sampled at the beginning of the experiment (day 0) and after 3, 7, and 14 days. BaP accumulation, in whole clam tissues, was analyzed. A multi-biomarker assessment was conducted in the gills, digestive gland, and haemolymph of clams to clarify the effects of exposure. This included the quantification of antioxidant (superoxide dismutase, catalase, glutathione peroxidase) and biotransformation (glutathione-Stransferases) enzyme activities, oxidative damage (lipid peroxidation levels), genotoxicity (single and double strand DNA breaks), and neurotoxicity (acetylcholinesterase activity). Results suggest a potential mechanical injury of gills caused by ingestion of microplastics that may also affect the analyzed biomarkers. The digestive gland seems less affected by mechanical damage caused by virgin microplastic exposure, with the MPs-adsorbed BaP and PFOS exerting a negative influence over the assessed biomarkers in this tissue.JPI Oceans FCT JPIOCEANS/0005/2015 JPI Oceans (BELSPO) JPI Oceans (FWO) JPI Oceans (FORMAS) JPI Oceans (SwAM) FCT UID/MAR/00350/2013Frontiers MediaSapientiaO'Donovan, SaritMestre, NéliaAbel, SerenaFonseca, TaináCarteny, Camilla C.Cormier, BettieKeiter, Steffen H.Bebianno, Maria João2019-04-04T14:08:41Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/12446eng2296-774510.3389/fmars.2018.00143info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:24:24Zoai:sapientia.ualg.pt:10400.1/12446Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:48.168295Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana |
title |
Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana |
spellingShingle |
Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana O'Donovan, Sarit Polyethylene Benzo[a]pyrene Perfluorooctane sulfonic acid DNA damage Oxidative stress Neurotoxicity Oxidative damage |
title_short |
Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana |
title_full |
Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana |
title_fullStr |
Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana |
title_full_unstemmed |
Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana |
title_sort |
Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana |
author |
O'Donovan, Sarit |
author_facet |
O'Donovan, Sarit Mestre, Nélia Abel, Serena Fonseca, Tainá Carteny, Camilla C. Cormier, Bettie Keiter, Steffen H. Bebianno, Maria João |
author_role |
author |
author2 |
Mestre, Nélia Abel, Serena Fonseca, Tainá Carteny, Camilla C. Cormier, Bettie Keiter, Steffen H. Bebianno, Maria João |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
O'Donovan, Sarit Mestre, Nélia Abel, Serena Fonseca, Tainá Carteny, Camilla C. Cormier, Bettie Keiter, Steffen H. Bebianno, Maria João |
dc.subject.por.fl_str_mv |
Polyethylene Benzo[a]pyrene Perfluorooctane sulfonic acid DNA damage Oxidative stress Neurotoxicity Oxidative damage |
topic |
Polyethylene Benzo[a]pyrene Perfluorooctane sulfonic acid DNA damage Oxidative stress Neurotoxicity Oxidative damage |
description |
Although microplastics (MPs) are distributed globally in the marine environment, a great deal of unknowns relating to their ecotoxicological effects on the marine biota remains. Due to their lipophilic nature, microplastics have the potential to adsorb persistent organic pollutants present in contaminated regions, which may increase their detrimental impact once assimilated by organisms. This study investigates the ecotoxicological effects of exposure to low-density polyethylene (LDPE) microplastics (11-13 beta m), with and without adsorbed contaminants (benzo[a]pyrene- BaP and perfluorooctane sulfonic acid-PFOS), in the peppery furrow shell clam, Scrobicularia plana. Environmentally relevant concentrations of contaminants (BaP-16.87 +/- 0.22 mu g g(-1) and PFOS-70.22 +/- 12.41 mu g g(-1)) were adsorbed to microplastics to evaluate the potential role of plastic particles as a source of chemical contamination once ingested. S. plana were exposed to microplastics, at a concentration of 1 mg L-1, in a water-sediment exposure setup for 14 days. Clams were sampled at the beginning of the experiment (day 0) and after 3, 7, and 14 days. BaP accumulation, in whole clam tissues, was analyzed. A multi-biomarker assessment was conducted in the gills, digestive gland, and haemolymph of clams to clarify the effects of exposure. This included the quantification of antioxidant (superoxide dismutase, catalase, glutathione peroxidase) and biotransformation (glutathione-Stransferases) enzyme activities, oxidative damage (lipid peroxidation levels), genotoxicity (single and double strand DNA breaks), and neurotoxicity (acetylcholinesterase activity). Results suggest a potential mechanical injury of gills caused by ingestion of microplastics that may also affect the analyzed biomarkers. The digestive gland seems less affected by mechanical damage caused by virgin microplastic exposure, with the MPs-adsorbed BaP and PFOS exerting a negative influence over the assessed biomarkers in this tissue. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z 2019-04-04T14:08:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/12446 |
url |
http://hdl.handle.net/10400.1/12446 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2296-7745 10.3389/fmars.2018.00143 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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