Origin of salt additive effect on solute partitioning in aqueous polyethylene glycol-8000–sodium sulfate two-phase system

• Autor(a) principal: Silva, Nuno R.
• Data de Publicação: 2014
• Outros Autores: Ferreira, Luísa A., Mikheeva, Larissa M., Teixeira, J. A., Zaslavsky, Boris Y.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/31485
• topic: PEG–sulfate aqueous two-phase systems; Salt additives; Ionic and non-ionic solutes partitioning; Hofmeister series; Relative hydrophobicity; Electrostatic properties; Science & Technology

Partitioning of a homologous series of dinitrophenylted (DNP-) amino acids with aliphatic side chains was examined in aqueous polyethylene glycol (PEG)-8000–sodium sulfate two-phase systems (ATPS) with the additives NaSCN, NaClO4, and NaH2PO4 at concentrations varied from 0.025 M up to 0.54 M. The differences between the relative hydrophobicities and electrostatic properties of the two phases in all ATPS were estimated. Partitioning of adenine, adenosine mono-, di- and tri-phosphates was also examined in all ATPSs, including those with NaCl additive. Partition coefficients for these compounds and for nonionic organic compounds previously reported [L.A. Ferreira, P. Parpot, J.A. Teixeira, L.M. Mikheeva, B.Y. Zaslavsky, J. Chromatogr. A 1220 (2012) 14.] were analyzed in terms of linear solvent regression relationship. The results obtained suggest that the effects of the salts additives are related to their influence on the water structure.