The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1

Detalhes bibliográficos
Autor(a) principal: Casal, Margarida
Data de Publicação: 1999
Outros Autores: Paiva, Sandra, Andrade, Raquel P., Gancedo, Carlos, Leão, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/2971
Resumo: A mutant of Saccharomyces cerevisiae deficient in the lactate-proton symport was isolated. Transformation of the mutant with a yeast genomic library allowed the isolation of the gene JEN1 that restored lactate transport. Disruption of JEN1 abolished uptake of lactate. The results indicate that, under the experimental conditions tested, no other monocarboxylate permease is able to efficiently transport lactate in S. cerevisiae.
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spelling The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1Science & TechnologyA mutant of Saccharomyces cerevisiae deficient in the lactate-proton symport was isolated. Transformation of the mutant with a yeast genomic library allowed the isolation of the gene JEN1 that restored lactate transport. Disruption of JEN1 abolished uptake of lactate. The results indicate that, under the experimental conditions tested, no other monocarboxylate permease is able to efficiently transport lactate in S. cerevisiae.We acknowledge the help of Maria J. Lafuente in the DNA sequencing and analysis and the critical reading of the manuscript by Juana M. Gancedo. This study was supported by Portuguese grant PRAXIS/2/2.1/BIO/1068/95.Work in the laboratory of C.G. was supported by grant PB97-1213-CO2-01 from the Spanish CICYT. M.C. had a FEBS short-term fellowship during the early stages of this work. S.P. received a fellowship from PRAXIS XXI-BM4229. R.P.A. received a fellowship from PRAXIS XXI-BD15737/98.American Society for Microbiology (ASM)Universidade do MinhoCasal, MargaridaPaiva, SandraAndrade, Raquel P.Gancedo, CarlosLeão, Cecília1999-011999-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/2971engCasal, M., Paiva, S., Andrade Raquel, P., Gancedo, C., & Leão, C. (1999). The Lactate-Proton Symport of Saccharomyces cerevisiae Is Encoded by JEN1. Journal of Bacteriology, 181(8), 2620-2623. doi: 10.1128/jb.181.8.2620-2623.19990021-91931098-553010.1128/JB.181.8.2620-2623.199910198029PMC93691https://journals.asm.org/doi/10.1128/JB.181.8.2620-2623.1999info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:39Zoai:repositorium.sdum.uminho.pt:1822/2971Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:10.667434Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1
title The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1
spellingShingle The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1
Casal, Margarida
Science & Technology
title_short The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1
title_full The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1
title_fullStr The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1
title_full_unstemmed The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1
title_sort The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1
author Casal, Margarida
author_facet Casal, Margarida
Paiva, Sandra
Andrade, Raquel P.
Gancedo, Carlos
Leão, Cecília
author_role author
author2 Paiva, Sandra
Andrade, Raquel P.
Gancedo, Carlos
Leão, Cecília
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Casal, Margarida
Paiva, Sandra
Andrade, Raquel P.
Gancedo, Carlos
Leão, Cecília
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description A mutant of Saccharomyces cerevisiae deficient in the lactate-proton symport was isolated. Transformation of the mutant with a yeast genomic library allowed the isolation of the gene JEN1 that restored lactate transport. Disruption of JEN1 abolished uptake of lactate. The results indicate that, under the experimental conditions tested, no other monocarboxylate permease is able to efficiently transport lactate in S. cerevisiae.
publishDate 1999
dc.date.none.fl_str_mv 1999-01
1999-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/2971
url https://hdl.handle.net/1822/2971
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Casal, M., Paiva, S., Andrade Raquel, P., Gancedo, C., & Leão, C. (1999). The Lactate-Proton Symport of Saccharomyces cerevisiae Is Encoded by JEN1. Journal of Bacteriology, 181(8), 2620-2623. doi: 10.1128/jb.181.8.2620-2623.1999
0021-9193
1098-5530
10.1128/JB.181.8.2620-2623.1999
10198029
PMC93691
https://journals.asm.org/doi/10.1128/JB.181.8.2620-2623.1999
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology (ASM)
publisher.none.fl_str_mv American Society for Microbiology (ASM)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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