Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/20.500.12207/6120 |
Resumo: | Centaurea is a genus of winter weeds with a similar life cycle and competitive traits, which occurs in small-grains production fields in the central-southern of the Iberian Peninsula. However, most of herbicides recommended for weed management in wheat show poor control of Centaurea species. This study summarizes the biology, herbicide tolerance to acetolactate synthase (ALS) inhibitors, and recommended chemical alternatives for the control of Centaurea species. Four species (C. cyanus L., C. diluta Aiton, C. melitensis L. and C. pullata L. subsp. baetica Talavera), taxonomically characterized, were found as the main important broadleaf weeds in small- grains production fields of the Iberian Peninsula. These species showed innate tolerance to tribenuron-methyl (TM), showing LD50 values (mortality of 50% of a population) higher than the field dose of TM (20 g ai ha 1). The order of tolerance was C. diluta (LD50 =702 g ha 1) ≫ C. pullata (LD50 =180 g ha 1) ≫ C. cyanus (LD50 =65 g ha 1) >C. melitensis (LD50 =32 g ha 1). Centaurea cyanus and C. melitensis presented higher foliar retention (150–180 μL herbicide solution), absorption (14–28%) and subsequent translocation (7–12%) of TM with respect to the other two species. Centaurea spp. plants were able to metabolize 14C-TM into non-toxic forms (hydroxylated OH-metsulfuron-methyl and conjugated-metsulfuron-methyl), with cytochrome P450 (Cyt-P450) monooxygenases being responsible for herbicide detoxification. Centaurea cyanus and C. mellitensis metabolized up to 25% of TM, while C. diluta and C. pullata metabolized more than 50% of the herbicide. Centaurea species showed 80–100% survival when treated with of florasulam, imazamox and/or metsulfuron-methyl, i.e., these weeds present cross-tolerance to ALS inhibitors. In contrast, auxin mimics herbicides (2,4-D, clopyralid, dicamba, fluroxypir and MCPA) efficiently controlled the four Centaurea species. In addition, the mixture of ALS-inhibitors and auxin mimics also proved to be an interesting alternative for the control of Centaurea. These results show that plants of the genus Centaurea found in the winter cereal fields of the Iberian Peninsula have an innate tolerance to TM and cross-resistance to other ALS-inhibiting herbicides, governed by reduced absorption and translocation, but mainly by the metabolization of the herbicide via Cyt-P450. |
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Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaureaAcetolactate synthaseCornflowerCyt-P450Maltese star-thistleNorth african knapweedTribenuron-methylCentaurea is a genus of winter weeds with a similar life cycle and competitive traits, which occurs in small-grains production fields in the central-southern of the Iberian Peninsula. However, most of herbicides recommended for weed management in wheat show poor control of Centaurea species. This study summarizes the biology, herbicide tolerance to acetolactate synthase (ALS) inhibitors, and recommended chemical alternatives for the control of Centaurea species. Four species (C. cyanus L., C. diluta Aiton, C. melitensis L. and C. pullata L. subsp. baetica Talavera), taxonomically characterized, were found as the main important broadleaf weeds in small- grains production fields of the Iberian Peninsula. These species showed innate tolerance to tribenuron-methyl (TM), showing LD50 values (mortality of 50% of a population) higher than the field dose of TM (20 g ai ha 1). The order of tolerance was C. diluta (LD50 =702 g ha 1) ≫ C. pullata (LD50 =180 g ha 1) ≫ C. cyanus (LD50 =65 g ha 1) >C. melitensis (LD50 =32 g ha 1). Centaurea cyanus and C. melitensis presented higher foliar retention (150–180 μL herbicide solution), absorption (14–28%) and subsequent translocation (7–12%) of TM with respect to the other two species. Centaurea spp. plants were able to metabolize 14C-TM into non-toxic forms (hydroxylated OH-metsulfuron-methyl and conjugated-metsulfuron-methyl), with cytochrome P450 (Cyt-P450) monooxygenases being responsible for herbicide detoxification. Centaurea cyanus and C. mellitensis metabolized up to 25% of TM, while C. diluta and C. pullata metabolized more than 50% of the herbicide. Centaurea species showed 80–100% survival when treated with of florasulam, imazamox and/or metsulfuron-methyl, i.e., these weeds present cross-tolerance to ALS inhibitors. In contrast, auxin mimics herbicides (2,4-D, clopyralid, dicamba, fluroxypir and MCPA) efficiently controlled the four Centaurea species. In addition, the mixture of ALS-inhibitors and auxin mimics also proved to be an interesting alternative for the control of Centaurea. These results show that plants of the genus Centaurea found in the winter cereal fields of the Iberian Peninsula have an innate tolerance to TM and cross-resistance to other ALS-inhibiting herbicides, governed by reduced absorption and translocation, but mainly by the metabolization of the herbicide via Cyt-P450.ELSEVIER2024-01-29T11:49:19Z2023-01-24T00:00:00Z2023-01-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/octet-streamhttps://hdl.handle.net/20.500.12207/6120eng1873-6424https://doi.org/10.1016/j.envpol.2023.121140Palma-Bautista, CandelarioVásquez-García, José G.Portugal, JoãoBastida, FernandoAlcántara-de la Cruz, RicardoOsuna-Ruiz, Maria D.Torra, JoelDe Prado, Rafaelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-07T09:10:11Zoai:repositorio.ipbeja.pt:20.500.12207/6120Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:59:24.794613Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea |
title |
Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea |
spellingShingle |
Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea Palma-Bautista, Candelario Acetolactate synthase Cornflower Cyt-P450 Maltese star-thistle North african knapweed Tribenuron-methyl |
title_short |
Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea |
title_full |
Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea |
title_fullStr |
Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea |
title_full_unstemmed |
Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea |
title_sort |
Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of centaurea |
author |
Palma-Bautista, Candelario |
author_facet |
Palma-Bautista, Candelario Vásquez-García, José G. Portugal, João Bastida, Fernando Alcántara-de la Cruz, Ricardo Osuna-Ruiz, Maria D. Torra, Joel De Prado, Rafael |
author_role |
author |
author2 |
Vásquez-García, José G. Portugal, João Bastida, Fernando Alcántara-de la Cruz, Ricardo Osuna-Ruiz, Maria D. Torra, Joel De Prado, Rafael |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Palma-Bautista, Candelario Vásquez-García, José G. Portugal, João Bastida, Fernando Alcántara-de la Cruz, Ricardo Osuna-Ruiz, Maria D. Torra, Joel De Prado, Rafael |
dc.subject.por.fl_str_mv |
Acetolactate synthase Cornflower Cyt-P450 Maltese star-thistle North african knapweed Tribenuron-methyl |
topic |
Acetolactate synthase Cornflower Cyt-P450 Maltese star-thistle North african knapweed Tribenuron-methyl |
description |
Centaurea is a genus of winter weeds with a similar life cycle and competitive traits, which occurs in small-grains production fields in the central-southern of the Iberian Peninsula. However, most of herbicides recommended for weed management in wheat show poor control of Centaurea species. This study summarizes the biology, herbicide tolerance to acetolactate synthase (ALS) inhibitors, and recommended chemical alternatives for the control of Centaurea species. Four species (C. cyanus L., C. diluta Aiton, C. melitensis L. and C. pullata L. subsp. baetica Talavera), taxonomically characterized, were found as the main important broadleaf weeds in small- grains production fields of the Iberian Peninsula. These species showed innate tolerance to tribenuron-methyl (TM), showing LD50 values (mortality of 50% of a population) higher than the field dose of TM (20 g ai ha 1). The order of tolerance was C. diluta (LD50 =702 g ha 1) ≫ C. pullata (LD50 =180 g ha 1) ≫ C. cyanus (LD50 =65 g ha 1) >C. melitensis (LD50 =32 g ha 1). Centaurea cyanus and C. melitensis presented higher foliar retention (150–180 μL herbicide solution), absorption (14–28%) and subsequent translocation (7–12%) of TM with respect to the other two species. Centaurea spp. plants were able to metabolize 14C-TM into non-toxic forms (hydroxylated OH-metsulfuron-methyl and conjugated-metsulfuron-methyl), with cytochrome P450 (Cyt-P450) monooxygenases being responsible for herbicide detoxification. Centaurea cyanus and C. mellitensis metabolized up to 25% of TM, while C. diluta and C. pullata metabolized more than 50% of the herbicide. Centaurea species showed 80–100% survival when treated with of florasulam, imazamox and/or metsulfuron-methyl, i.e., these weeds present cross-tolerance to ALS inhibitors. In contrast, auxin mimics herbicides (2,4-D, clopyralid, dicamba, fluroxypir and MCPA) efficiently controlled the four Centaurea species. In addition, the mixture of ALS-inhibitors and auxin mimics also proved to be an interesting alternative for the control of Centaurea. These results show that plants of the genus Centaurea found in the winter cereal fields of the Iberian Peninsula have an innate tolerance to TM and cross-resistance to other ALS-inhibiting herbicides, governed by reduced absorption and translocation, but mainly by the metabolization of the herbicide via Cyt-P450. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-24T00:00:00Z 2023-01-24 2024-01-29T11:49:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12207/6120 |
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https://hdl.handle.net/20.500.12207/6120 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1873-6424 https://doi.org/10.1016/j.envpol.2023.121140 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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ELSEVIER |
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ELSEVIER |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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