Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/43756 |
Resumo: | Background: The incidence of melanoma is increasing worldwide. Since metastatic melanoma is highly aggressive, it is important to decipher all the biological aspects of melanoma cells. In this context, we have previously shown that metastatic FEMX-I melanoma cells release small (< 150 nm) extracellular vesicles (EVs) known as exosomes and ectosomes containing the stem (and cancer stem) cell antigenic marker CD133. EVs play an important role in intercellular communication, which could have a micro-environmental impact on surrounding tissues. Results: We report here a new type of large CD133+ EVs released by FEMX-I cells. Their sizes range from 2 to 6 µm and they contain lipid droplets and mitochondria. Real-time video microscopy revealed that these EVs originate from the lipid droplet-enriched cell extremities that did not completely retract during the cell division process. Once released, they can be taken up by other cells. Silencing CD133 significantly affected the cellular distribution of lipid droplets, with a re-localization around the nuclear compartment. As a result, the formation of large EVs containing lipid droplets was severely compromised. Conclusion: Given the biochemical effect of lipid droplets and mitochondria and/or their complexes on cell metabolism, the release and uptake of these new large CD133+ EVs from dividing aggressive melanoma cells can influence both donor and recipient cells, and therefore impact melanoma growth and dissemination. |
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Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell divisionCell divisionExtracellular vesicleLipid dropletMelanomaMitochondrionProminin-1Background: The incidence of melanoma is increasing worldwide. Since metastatic melanoma is highly aggressive, it is important to decipher all the biological aspects of melanoma cells. In this context, we have previously shown that metastatic FEMX-I melanoma cells release small (< 150 nm) extracellular vesicles (EVs) known as exosomes and ectosomes containing the stem (and cancer stem) cell antigenic marker CD133. EVs play an important role in intercellular communication, which could have a micro-environmental impact on surrounding tissues. Results: We report here a new type of large CD133+ EVs released by FEMX-I cells. Their sizes range from 2 to 6 µm and they contain lipid droplets and mitochondria. Real-time video microscopy revealed that these EVs originate from the lipid droplet-enriched cell extremities that did not completely retract during the cell division process. Once released, they can be taken up by other cells. Silencing CD133 significantly affected the cellular distribution of lipid droplets, with a re-localization around the nuclear compartment. As a result, the formation of large EVs containing lipid droplets was severely compromised. Conclusion: Given the biochemical effect of lipid droplets and mitochondria and/or their complexes on cell metabolism, the release and uptake of these new large CD133+ EVs from dividing aggressive melanoma cells can influence both donor and recipient cells, and therefore impact melanoma growth and dissemination.Veritati - Repositório Institucional da Universidade Católica PortuguesaKarbanová, JanaDeniz, Ilker A.Wilsch-Bräuninger, MichaelaCouto, Rita Alexandra de SousaFargeas, Christine A.Santos, Mark F.Lorico, AurelioCorbeil, Denis2024-01-29T17:30:48Z2024-122024-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/43756eng1478-811X10.1186/s12964-024-01471-785182719190PMC1079937338243233001146019500003info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-13T01:33:52Zoai:repositorio.ucp.pt:10400.14/43756Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:58:51.668559Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division |
title |
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division |
spellingShingle |
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division Karbanová, Jana Cell division Extracellular vesicle Lipid droplet Melanoma Mitochondrion Prominin-1 |
title_short |
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division |
title_full |
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division |
title_fullStr |
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division |
title_full_unstemmed |
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division |
title_sort |
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division |
author |
Karbanová, Jana |
author_facet |
Karbanová, Jana Deniz, Ilker A. Wilsch-Bräuninger, Michaela Couto, Rita Alexandra de Sousa Fargeas, Christine A. Santos, Mark F. Lorico, Aurelio Corbeil, Denis |
author_role |
author |
author2 |
Deniz, Ilker A. Wilsch-Bräuninger, Michaela Couto, Rita Alexandra de Sousa Fargeas, Christine A. Santos, Mark F. Lorico, Aurelio Corbeil, Denis |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Karbanová, Jana Deniz, Ilker A. Wilsch-Bräuninger, Michaela Couto, Rita Alexandra de Sousa Fargeas, Christine A. Santos, Mark F. Lorico, Aurelio Corbeil, Denis |
dc.subject.por.fl_str_mv |
Cell division Extracellular vesicle Lipid droplet Melanoma Mitochondrion Prominin-1 |
topic |
Cell division Extracellular vesicle Lipid droplet Melanoma Mitochondrion Prominin-1 |
description |
Background: The incidence of melanoma is increasing worldwide. Since metastatic melanoma is highly aggressive, it is important to decipher all the biological aspects of melanoma cells. In this context, we have previously shown that metastatic FEMX-I melanoma cells release small (< 150 nm) extracellular vesicles (EVs) known as exosomes and ectosomes containing the stem (and cancer stem) cell antigenic marker CD133. EVs play an important role in intercellular communication, which could have a micro-environmental impact on surrounding tissues. Results: We report here a new type of large CD133+ EVs released by FEMX-I cells. Their sizes range from 2 to 6 µm and they contain lipid droplets and mitochondria. Real-time video microscopy revealed that these EVs originate from the lipid droplet-enriched cell extremities that did not completely retract during the cell division process. Once released, they can be taken up by other cells. Silencing CD133 significantly affected the cellular distribution of lipid droplets, with a re-localization around the nuclear compartment. As a result, the formation of large EVs containing lipid droplets was severely compromised. Conclusion: Given the biochemical effect of lipid droplets and mitochondria and/or their complexes on cell metabolism, the release and uptake of these new large CD133+ EVs from dividing aggressive melanoma cells can influence both donor and recipient cells, and therefore impact melanoma growth and dissemination. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-01-29T17:30:48Z 2024-12 2024-12-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/43756 |
url |
http://hdl.handle.net/10400.14/43756 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1478-811X 10.1186/s12964-024-01471-7 85182719190 PMC10799373 38243233 001146019500003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137072733224960 |