The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Fernando José dos Santos
Data de Publicação: 2003
Outros Autores: Ludovico, Paula, Sousa, Maria João, Steensma, H. Yde, Côrte-Real, Manuela, Leão, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1711
Resumo: Zygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants. Mitotic chromosome loss induced by benomyl and selection on canavanine media resulted in three haploid strains of Z. bailii. This new set of Z. bailii strains allows the easy isolation of recessive mutants and is suitable for further molecular genetic studies.
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spelling The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidizationYeastMytotic sporesScience & TechnologyZygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants. Mitotic chromosome loss induced by benomyl and selection on canavanine media resulted in three haploid strains of Z. bailii. This new set of Z. bailii strains allows the easy isolation of recessive mutants and is suitable for further molecular genetic studies.Fundação para a Ciência e a Tecnologia (FCT) – Praxis XXI P/AGR/11135/98.American Society for Microbiology (ASM)Universidade do MinhoRodrigues, Fernando José dos SantosLudovico, PaulaSousa, Maria JoãoSteensma, H. YdeCôrte-Real, ManuelaLeão, Cecília2003-012003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1711eng"Applied and Environmental Microbiology". ISSN 0099-2240. 69:1 (2003) 649-653.1098-533610.1128/AEM.69.1.649-653.200312514054http://aem.asm.org/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:58:56Zoai:repositorium.sdum.uminho.pt:1822/1711Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:42.525105Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization
title The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization
spellingShingle The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization
Rodrigues, Fernando José dos Santos
Yeast
Mytotic spores
Science & Technology
title_short The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization
title_full The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization
title_fullStr The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization
title_full_unstemmed The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization
title_sort The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization
author Rodrigues, Fernando José dos Santos
author_facet Rodrigues, Fernando José dos Santos
Ludovico, Paula
Sousa, Maria João
Steensma, H. Yde
Côrte-Real, Manuela
Leão, Cecília
author_role author
author2 Ludovico, Paula
Sousa, Maria João
Steensma, H. Yde
Côrte-Real, Manuela
Leão, Cecília
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, Fernando José dos Santos
Ludovico, Paula
Sousa, Maria João
Steensma, H. Yde
Côrte-Real, Manuela
Leão, Cecília
dc.subject.por.fl_str_mv Yeast
Mytotic spores
Science & Technology
topic Yeast
Mytotic spores
Science & Technology
description Zygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants. Mitotic chromosome loss induced by benomyl and selection on canavanine media resulted in three haploid strains of Z. bailii. This new set of Z. bailii strains allows the easy isolation of recessive mutants and is suitable for further molecular genetic studies.
publishDate 2003
dc.date.none.fl_str_mv 2003-01
2003-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1711
url http://hdl.handle.net/1822/1711
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Applied and Environmental Microbiology". ISSN 0099-2240. 69:1 (2003) 649-653.
1098-5336
10.1128/AEM.69.1.649-653.2003
12514054
http://aem.asm.org/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology (ASM)
publisher.none.fl_str_mv American Society for Microbiology (ASM)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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