Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria

Detalhes bibliográficos
Autor(a) principal: Anand, Paras K.
Data de Publicação: 2010
Outros Autores: Anand, Ellis, Bleck, Christopher K. E., Anes, Elsa, Griffiths, Gareth
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/5453
Resumo: © 2010 Anand et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteriaExosomesHsp70InflammationMycobacteriaMycobacterial infection© 2010 Anand et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Background: Exosomes are endosome-derived vesicles that are released when multi-vesicular bodies (MVBs) fuse with the plasma membrane. Exosomes released from mycobacteria-infected cells have recently been shown to be pro-inflammatory. A prominent host molecule that is found within these exosomes is Hsp70, a member of the heat-shock family of proteins. Methodology/Principal Findings: We first characterized the exosomes purified from control and mycobacteria-infected cells. We found that relative to uninfected cells, macrophages infected with M. smegmatis and M. avium release more exosomes and the exosomes they released had more Hsp70 on their surface. Both exosomes and exogenous Hsp70 treatment of macrophages led to NF-kB activation and TNFa release in uninfected macrophages; Hsp70 levels were elevated in mycobacteria-infected cells. Macrophage treatment with Hsp70 also led to increase in the phagocytosis and maturation of latex-bead phagosomes. Finally, Hsp70 pre-incubation of M. smegmatis- and M. avium-infected cells led to increased phago-lysosome fusion, as well as more killing of mycobacteria within macrophages. Conclusions/Significance: Our results fit into an emerging concept whereby exosomes-containing Hsp70 are effective inducers of inflammation, also in response to mycobacterial infection.E.A. was supported by Fundação para a Ciência e a Tecnologia (FCT) Grant PIC/IC/82859/2007 and PTDC/SAU-MII/098024/2008. P.K.A was supported by a post-doctoral research grant from Alexander von Humboldt foundation, Germany and Marie-Curie fellowship from European Union, FP6 programme MIF1-CT- 2006-039351. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of ScienceRepositório da Universidade de LisboaAnand, Paras K.Anand, EllisBleck, Christopher K. E.Anes, ElsaGriffiths, Gareth2012-02-28T16:09:01Z2010-042010-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/5453engPLoS ONE | April 2010 | Volume 5 | Issue 4 | e101361932-6203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:47:07Zoai:repositorio.ul.pt:10451/5453Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:30:47.263786Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
title Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
spellingShingle Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
Anand, Paras K.
Exosomes
Hsp70
Inflammation
Mycobacteria
Mycobacterial infection
title_short Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
title_full Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
title_fullStr Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
title_full_unstemmed Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
title_sort Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
author Anand, Paras K.
author_facet Anand, Paras K.
Anand, Ellis
Bleck, Christopher K. E.
Anes, Elsa
Griffiths, Gareth
author_role author
author2 Anand, Ellis
Bleck, Christopher K. E.
Anes, Elsa
Griffiths, Gareth
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Anand, Paras K.
Anand, Ellis
Bleck, Christopher K. E.
Anes, Elsa
Griffiths, Gareth
dc.subject.por.fl_str_mv Exosomes
Hsp70
Inflammation
Mycobacteria
Mycobacterial infection
topic Exosomes
Hsp70
Inflammation
Mycobacteria
Mycobacterial infection
description © 2010 Anand et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
2010-04-01T00:00:00Z
2012-02-28T16:09:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/5453
url http://hdl.handle.net/10451/5453
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS ONE | April 2010 | Volume 5 | Issue 4 | e10136
1932-6203
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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