Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5397 https://doi.org/10.1016/S0887-2333(03)00090-0 |
Resumo: | Microorganisms are very powerful tools for the supply of information about the toxic effects of lipophilic compounds, since an impairment of cell growth usually occurs as a result of perturbations related, in most cases, with the partition of toxicants in membranes. The thermophilic eubacterium Bacillus stearothermophilus has been used as a model system to identify [alpha]- and [beta]-endosulfan interactions with the membrane possibly related with the insecticide toxicity. Two approaches have been pursued: (a) bacterial growth is followed and the effects of endosulfan isomers determined; (b) biophysical studies with the fluorescent fluidity probe 1,6-diphenyl-1,3,5-hexatriene (DPH) were performed to assess the effects of [alpha]- and [beta]-endosulfan on the organization of the membrane lipid bilayer. The effects on growth were quantitatively evaluated by determination of growth parameters, namely the lag phase, the specific growth rate and the cell density reached by cultures in the stationary phase. Growth inhibition by [alpha] and [beta]-endosulfan dependent on the concentration is diminished or removed by the addition of 2.5 m Ca2+ to bacterial cultures. Fluorescence DPH polarization consistently showed opposite effects of Ca2+ and [alpha]- and [beta]-endosulfan on the physical state of bacterial polar lipid dispersions. |
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Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan[alpha]-Endosulfan[beta]-EndosulfanFluorescence polarizationBacillus stearothermophilusBacterial growthLipid membraneMicroorganisms are very powerful tools for the supply of information about the toxic effects of lipophilic compounds, since an impairment of cell growth usually occurs as a result of perturbations related, in most cases, with the partition of toxicants in membranes. The thermophilic eubacterium Bacillus stearothermophilus has been used as a model system to identify [alpha]- and [beta]-endosulfan interactions with the membrane possibly related with the insecticide toxicity. Two approaches have been pursued: (a) bacterial growth is followed and the effects of endosulfan isomers determined; (b) biophysical studies with the fluorescent fluidity probe 1,6-diphenyl-1,3,5-hexatriene (DPH) were performed to assess the effects of [alpha]- and [beta]-endosulfan on the organization of the membrane lipid bilayer. The effects on growth were quantitatively evaluated by determination of growth parameters, namely the lag phase, the specific growth rate and the cell density reached by cultures in the stationary phase. Growth inhibition by [alpha] and [beta]-endosulfan dependent on the concentration is diminished or removed by the addition of 2.5 m Ca2+ to bacterial cultures. Fluorescence DPH polarization consistently showed opposite effects of Ca2+ and [alpha]- and [beta]-endosulfan on the physical state of bacterial polar lipid dispersions.http://www.sciencedirect.com/science/article/B6TCP-49H6XR8-1/1/147369f84a1c06432bbf002d58304bb72003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5397http://hdl.handle.net/10316/5397https://doi.org/10.1016/S0887-2333(03)00090-0engToxicology in Vitro. 17:5-6 (2003) 595-601Martins, J. D.Monteiro, J. P.Antunes-Madeira, M. C.Jurado, A. S.Madeira, V. M. C.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-22T11:40:37Zoai:estudogeral.uc.pt:10316/5397Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:28.580428Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan |
title |
Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan |
spellingShingle |
Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan Martins, J. D. [alpha]-Endosulfan [beta]-Endosulfan Fluorescence polarization Bacillus stearothermophilus Bacterial growth Lipid membrane |
title_short |
Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan |
title_full |
Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan |
title_fullStr |
Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan |
title_full_unstemmed |
Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan |
title_sort |
Use of the microorganism Bacillus stearothermophilus as a model to evaluate toxicity of the lipophilic environmental pollutant endosulfan |
author |
Martins, J. D. |
author_facet |
Martins, J. D. Monteiro, J. P. Antunes-Madeira, M. C. Jurado, A. S. Madeira, V. M. C. |
author_role |
author |
author2 |
Monteiro, J. P. Antunes-Madeira, M. C. Jurado, A. S. Madeira, V. M. C. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Martins, J. D. Monteiro, J. P. Antunes-Madeira, M. C. Jurado, A. S. Madeira, V. M. C. |
dc.subject.por.fl_str_mv |
[alpha]-Endosulfan [beta]-Endosulfan Fluorescence polarization Bacillus stearothermophilus Bacterial growth Lipid membrane |
topic |
[alpha]-Endosulfan [beta]-Endosulfan Fluorescence polarization Bacillus stearothermophilus Bacterial growth Lipid membrane |
description |
Microorganisms are very powerful tools for the supply of information about the toxic effects of lipophilic compounds, since an impairment of cell growth usually occurs as a result of perturbations related, in most cases, with the partition of toxicants in membranes. The thermophilic eubacterium Bacillus stearothermophilus has been used as a model system to identify [alpha]- and [beta]-endosulfan interactions with the membrane possibly related with the insecticide toxicity. Two approaches have been pursued: (a) bacterial growth is followed and the effects of endosulfan isomers determined; (b) biophysical studies with the fluorescent fluidity probe 1,6-diphenyl-1,3,5-hexatriene (DPH) were performed to assess the effects of [alpha]- and [beta]-endosulfan on the organization of the membrane lipid bilayer. The effects on growth were quantitatively evaluated by determination of growth parameters, namely the lag phase, the specific growth rate and the cell density reached by cultures in the stationary phase. Growth inhibition by [alpha] and [beta]-endosulfan dependent on the concentration is diminished or removed by the addition of 2.5 m Ca2+ to bacterial cultures. Fluorescence DPH polarization consistently showed opposite effects of Ca2+ and [alpha]- and [beta]-endosulfan on the physical state of bacterial polar lipid dispersions. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5397 http://hdl.handle.net/10316/5397 https://doi.org/10.1016/S0887-2333(03)00090-0 |
url |
http://hdl.handle.net/10316/5397 https://doi.org/10.1016/S0887-2333(03)00090-0 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology in Vitro. 17:5-6 (2003) 595-601 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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