Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/8805 |
Resumo: | The present study aims to find a method to reduce Staphylococcus epidermidis adhesion to acrylic and silicone – two materials used commonly in medical devices – by heparin and gentian violet surface preconditioning. Different periods of heparin preconditioning are studied to evaluate the influence of preincubation time on the reduction of bacterial adhesion. A two-hour period was chosen and applied in the adhesion assays with either heparin or gentian violet. Squares of the materials with adherent cells were also analysed by scanning electron microscopy (SEM). Results of adhesion assays showed a significant reduction (53–90%, P<0.05) in bacterial adhesion to silicone and acrylic after precontact with the conditioning substances. No statistical differences (P>0.05) were found between the extent of adhesion on silicone coupons precontacted either with heparin or gentian violet for each of the strains tested. On acrylic, heparin was more efficient (P<0.001) in reducing S. epidermidis IE186 adhesion than was gentian violet (85% and 53% reductions, respectively). Therefore, immersion of acrylic and silicone in heparin or gentian violet may constitute a simple and effective method by which to reduce S. epidermidis adhesion to medical devices. |
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Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedureCross infectionGentian violetHeparinStaphylococcus epidermidisScience & TechnologyThe present study aims to find a method to reduce Staphylococcus epidermidis adhesion to acrylic and silicone – two materials used commonly in medical devices – by heparin and gentian violet surface preconditioning. Different periods of heparin preconditioning are studied to evaluate the influence of preincubation time on the reduction of bacterial adhesion. A two-hour period was chosen and applied in the adhesion assays with either heparin or gentian violet. Squares of the materials with adherent cells were also analysed by scanning electron microscopy (SEM). Results of adhesion assays showed a significant reduction (53–90%, P<0.05) in bacterial adhesion to silicone and acrylic after precontact with the conditioning substances. No statistical differences (P>0.05) were found between the extent of adhesion on silicone coupons precontacted either with heparin or gentian violet for each of the strains tested. On acrylic, heparin was more efficient (P<0.001) in reducing S. epidermidis IE186 adhesion than was gentian violet (85% and 53% reductions, respectively). Therefore, immersion of acrylic and silicone in heparin or gentian violet may constitute a simple and effective method by which to reduce S. epidermidis adhesion to medical devices.Fundação para a Ciência e a Tecnologia (FCT) - projecto POCTI/ESP/42688/2001, SFRH/BD/19265/2004 and SFRH/BPD/26803/2006Institute of Biomedical ScienceUniversidade do MinhoSousa, CláudiaHenriques, MarianaTeixeira, P.Oliveira, Rosário20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/8805eng"British Journal of Biomedical Science." ISSN 0967-4845. 65:4 (2008) 184-190.0967-484510.1080/09674845.2008.1173282619181036info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:29:45Zoai:repositorium.sdum.uminho.pt:1822/8805Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:29:45Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure |
title |
Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure |
spellingShingle |
Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure Sousa, Cláudia Cross infection Gentian violet Heparin Staphylococcus epidermidis Science & Technology |
title_short |
Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure |
title_full |
Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure |
title_fullStr |
Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure |
title_full_unstemmed |
Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure |
title_sort |
Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices : a simple procedure |
author |
Sousa, Cláudia |
author_facet |
Sousa, Cláudia Henriques, Mariana Teixeira, P. Oliveira, Rosário |
author_role |
author |
author2 |
Henriques, Mariana Teixeira, P. Oliveira, Rosário |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sousa, Cláudia Henriques, Mariana Teixeira, P. Oliveira, Rosário |
dc.subject.por.fl_str_mv |
Cross infection Gentian violet Heparin Staphylococcus epidermidis Science & Technology |
topic |
Cross infection Gentian violet Heparin Staphylococcus epidermidis Science & Technology |
description |
The present study aims to find a method to reduce Staphylococcus epidermidis adhesion to acrylic and silicone – two materials used commonly in medical devices – by heparin and gentian violet surface preconditioning. Different periods of heparin preconditioning are studied to evaluate the influence of preincubation time on the reduction of bacterial adhesion. A two-hour period was chosen and applied in the adhesion assays with either heparin or gentian violet. Squares of the materials with adherent cells were also analysed by scanning electron microscopy (SEM). Results of adhesion assays showed a significant reduction (53–90%, P<0.05) in bacterial adhesion to silicone and acrylic after precontact with the conditioning substances. No statistical differences (P>0.05) were found between the extent of adhesion on silicone coupons precontacted either with heparin or gentian violet for each of the strains tested. On acrylic, heparin was more efficient (P<0.001) in reducing S. epidermidis IE186 adhesion than was gentian violet (85% and 53% reductions, respectively). Therefore, immersion of acrylic and silicone in heparin or gentian violet may constitute a simple and effective method by which to reduce S. epidermidis adhesion to medical devices. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/8805 |
url |
http://hdl.handle.net/1822/8805 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"British Journal of Biomedical Science." ISSN 0967-4845. 65:4 (2008) 184-190. 0967-4845 10.1080/09674845.2008.11732826 19181036 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Biomedical Science |
publisher.none.fl_str_mv |
Institute of Biomedical Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545337546473472 |