Differential expression and co-regulation of carrot AOX genes (Daucus carota)
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/2736 |
Resumo: | Alternative oxidase (AOX) is a mitochondrial protein encoded by the nuclear genome. In higher plants AOX genes form a small multigene family mostly consisting of the two subfamilies AOX1 and AOX2. Daucus carota L. is characterized by a unique extension pattern of AOX genes. Different from other plant species studied so far it contains two genes in both subfamilies. Therefore, carrot was recently highlighted as an important model in AOX stress research to understand the evolutionary importance of both AOX subfamilies. Here we report on the expression patterns of DcAOX1a, DcAOX1b and DcAOX2a and DcAOX2b. Our results demonstrate that all of the four carrot AOX genes are expressed. Differential expression was observed in organs, tissues and during de novo induction of secondary root phloem explants to growth and development. DcAOX1a and DcAOX2a indicated a differential transcript accumulation but a similar co-expression pattern. The genes of each carrot AOX sub-family revealed a differential regulation and responsiveness. DcAOX2a indicated high inducibility in contrast to DcAOX2b, which generally revealed low transcript abundance and rather weak responses. In search for withingene sequence differences between both genes as a potential reason for the differential expression patterns, the structural organization of the two genes was compared. DcAOX2a and DcAOX2b showed high sequence similarity in their open reading frames (ORFs). However, length variability was observed in the N-terminal exon1 region. The predicted cleavage site of the mitochondrial targeting sequence in this locus is untypical small for both genes and consists of 35 amino acids for DcAOX2a and of 21 amino acids for DcAOX2b. The importance of structural gene organization and the relevancy of within-gene sequence variations are discussed. Our results strengthen the value of carrot as a model plant for future studies on the importance of AOX sub family evolution. |
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Differential expression and co-regulation of carrot AOX genes (Daucus carota)--Alternative oxidase (AOX) is a mitochondrial protein encoded by the nuclear genome. In higher plants AOX genes form a small multigene family mostly consisting of the two subfamilies AOX1 and AOX2. Daucus carota L. is characterized by a unique extension pattern of AOX genes. Different from other plant species studied so far it contains two genes in both subfamilies. Therefore, carrot was recently highlighted as an important model in AOX stress research to understand the evolutionary importance of both AOX subfamilies. Here we report on the expression patterns of DcAOX1a, DcAOX1b and DcAOX2a and DcAOX2b. Our results demonstrate that all of the four carrot AOX genes are expressed. Differential expression was observed in organs, tissues and during de novo induction of secondary root phloem explants to growth and development. DcAOX1a and DcAOX2a indicated a differential transcript accumulation but a similar co-expression pattern. The genes of each carrot AOX sub-family revealed a differential regulation and responsiveness. DcAOX2a indicated high inducibility in contrast to DcAOX2b, which generally revealed low transcript abundance and rather weak responses. In search for withingene sequence differences between both genes as a potential reason for the differential expression patterns, the structural organization of the two genes was compared. DcAOX2a and DcAOX2b showed high sequence similarity in their open reading frames (ORFs). However, length variability was observed in the N-terminal exon1 region. The predicted cleavage site of the mitochondrial targeting sequence in this locus is untypical small for both genes and consists of 35 amino acids for DcAOX2a and of 21 amino acids for DcAOX2b. The importance of structural gene organization and the relevancy of within-gene sequence variations are discussed. Our results strengthen the value of carrot as a model plant for future studies on the importance of AOX sub family evolution.Wiley2011-08-17T15:02:39Z2011-08-172009-08-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9451 bytesapplication/pdfhttp://hdl.handle.net/10174/2736http://hdl.handle.net/10174/2736por678-6910031-9317 (print) 1399-3054 (online)137Physiologia Plantarum4restrito_uendhcardoso@uevora.ptndndndndndeu_chair@uevora.pt577Campos, Maria DoroteiaCardoso, HéliaLinke, BettinaCosta, José HélioFernandes de Melo, DirceJusto, LígiaFrederico, António MiguelArnholdt-Schmitt, Birgitinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:38:04Zoai:dspace.uevora.pt:10174/2736Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:57:47.180104Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differential expression and co-regulation of carrot AOX genes (Daucus carota) |
title |
Differential expression and co-regulation of carrot AOX genes (Daucus carota) |
spellingShingle |
Differential expression and co-regulation of carrot AOX genes (Daucus carota) Campos, Maria Doroteia - - |
title_short |
Differential expression and co-regulation of carrot AOX genes (Daucus carota) |
title_full |
Differential expression and co-regulation of carrot AOX genes (Daucus carota) |
title_fullStr |
Differential expression and co-regulation of carrot AOX genes (Daucus carota) |
title_full_unstemmed |
Differential expression and co-regulation of carrot AOX genes (Daucus carota) |
title_sort |
Differential expression and co-regulation of carrot AOX genes (Daucus carota) |
author |
Campos, Maria Doroteia |
author_facet |
Campos, Maria Doroteia Cardoso, Hélia Linke, Bettina Costa, José Hélio Fernandes de Melo, Dirce Justo, Lígia Frederico, António Miguel Arnholdt-Schmitt, Birgit |
author_role |
author |
author2 |
Cardoso, Hélia Linke, Bettina Costa, José Hélio Fernandes de Melo, Dirce Justo, Lígia Frederico, António Miguel Arnholdt-Schmitt, Birgit |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Campos, Maria Doroteia Cardoso, Hélia Linke, Bettina Costa, José Hélio Fernandes de Melo, Dirce Justo, Lígia Frederico, António Miguel Arnholdt-Schmitt, Birgit |
dc.subject.por.fl_str_mv |
- - |
topic |
- - |
description |
Alternative oxidase (AOX) is a mitochondrial protein encoded by the nuclear genome. In higher plants AOX genes form a small multigene family mostly consisting of the two subfamilies AOX1 and AOX2. Daucus carota L. is characterized by a unique extension pattern of AOX genes. Different from other plant species studied so far it contains two genes in both subfamilies. Therefore, carrot was recently highlighted as an important model in AOX stress research to understand the evolutionary importance of both AOX subfamilies. Here we report on the expression patterns of DcAOX1a, DcAOX1b and DcAOX2a and DcAOX2b. Our results demonstrate that all of the four carrot AOX genes are expressed. Differential expression was observed in organs, tissues and during de novo induction of secondary root phloem explants to growth and development. DcAOX1a and DcAOX2a indicated a differential transcript accumulation but a similar co-expression pattern. The genes of each carrot AOX sub-family revealed a differential regulation and responsiveness. DcAOX2a indicated high inducibility in contrast to DcAOX2b, which generally revealed low transcript abundance and rather weak responses. In search for withingene sequence differences between both genes as a potential reason for the differential expression patterns, the structural organization of the two genes was compared. DcAOX2a and DcAOX2b showed high sequence similarity in their open reading frames (ORFs). However, length variability was observed in the N-terminal exon1 region. The predicted cleavage site of the mitochondrial targeting sequence in this locus is untypical small for both genes and consists of 35 amino acids for DcAOX2a and of 21 amino acids for DcAOX2b. The importance of structural gene organization and the relevancy of within-gene sequence variations are discussed. Our results strengthen the value of carrot as a model plant for future studies on the importance of AOX sub family evolution. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-08-24T00:00:00Z 2011-08-17T15:02:39Z 2011-08-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/2736 http://hdl.handle.net/10174/2736 |
url |
http://hdl.handle.net/10174/2736 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
678-691 0031-9317 (print) 1399-3054 (online) 137 Physiologia Plantarum 4 restrito_ue nd hcardoso@uevora.pt nd nd nd nd nd eu_chair@uevora.pt 577 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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9451 bytes application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
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