Identificação de alelos S em diferentes cultivares de pereira e avaliação da sua compatibilidade gametofítica com a 'Rocha'

• Autor(a) principal: Oliveira, Cristina Moniz
• Data de Publicação: 2009
• Outros Autores: Mota, Mariana, Tavares, Lucélia R.
• Idioma: por
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.5/2369
• topic: Pyrus communis; S-RNase; gametophytic self-incompatibility; cross pollination; PCR; polinização cruzada; incompatibilidade gametofitica

In order to select pear cultivar which are genetically compatible with ‘Rocha’ (Sa, Sj), the identification of S alleles sequences of commercial cultivars with bloom periods overlapping (partially or totally) the bloom period of ‘Rocha’ was performed. Partial sequences of the S alleles from the different cultivars were amplified with specific primers, cloned and sequenced. ‘Pêra De Água’ (S22, Sb) ‘Général Leclerc’ (Sl, Sq) and ‘Alexandrine Douillard’ (Sb, Sk) should be totally compatible. It was identified a Sj allele in the cultivars ‘Amêndoa’, ‘Beurré Precoce Morettini’, ‘Clapp’s Rouge’ e ‘Clapp’s Favourite’ and an allele Sa in ‘Beurré Clairgeau’, indicating that these cultivars are semi-compatible with ‘Rocha’. ‘Carapinheira’ (Sb) and ‘Pérola’ (Sk) carry a different allele from the ones of ‘Rocha’ and an amplification pattern completely dissimilar suggesting full compatibility. In ‘Passe Crassane’ (Sr) and ‘Beurré D’Avril’ (S4), a different allele from the S alleles of ‘Rocha’ was identified indicating compatibility, but total or partial compatibility remained unclear, since the amplification pattern does not exclude that the unknown allele might be Sa from ‘Rocha’.