Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/90895 |
Resumo: | Coagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis, are one of the leading causes of sepsis in preterm neonates mostly due to their capacity to form biofilms on medical devices. A major concern regarding CoNS infections is associated with the increased antibiotic resistance that has been observed among this bacterial group. Hence, the treatment of these infections is becoming progressively challenging, which may lead to increased morbidity and mortality in preterm neonates. In that sense, we aim to explore the consequences of antibiotic resistance in biofilm formation by S. epidermidis. First, the susceptibility profile of S. epidermidis strains collected from Portuguese newborns with bloodstream infections was characterized. To induce resistance to vancomycin, the susceptible strains were exposed to increasing concentrations of the antibiotic and, after 36 passages, the MIC was re-evaluated and the capacity of vancomycin-induced resistance strains to form biofilms was assessed (Colony forming units (CFUs) and OD620nm readings). Overall, the strains with induced resistance to vancomycin formed biofilms with less biomass and CFUs than the isogenic strains. The acquisition of resistance to vancomycin in clinical strains of S. epidermidis seems to cause a decrease in the capacity of these strains to form biofilms. As such, in S. epidermidis, the resistance to vancomycin does not seem to be related to a stronger biofilm formation capacity. |
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Induced resistance to vancomycin impairs biofilm formation by S. epidermidisStaphylococcus epidermidisBiofilmsAntibiotic resistanceVancomycinCoagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis, are one of the leading causes of sepsis in preterm neonates mostly due to their capacity to form biofilms on medical devices. A major concern regarding CoNS infections is associated with the increased antibiotic resistance that has been observed among this bacterial group. Hence, the treatment of these infections is becoming progressively challenging, which may lead to increased morbidity and mortality in preterm neonates. In that sense, we aim to explore the consequences of antibiotic resistance in biofilm formation by S. epidermidis. First, the susceptibility profile of S. epidermidis strains collected from Portuguese newborns with bloodstream infections was characterized. To induce resistance to vancomycin, the susceptible strains were exposed to increasing concentrations of the antibiotic and, after 36 passages, the MIC was re-evaluated and the capacity of vancomycin-induced resistance strains to form biofilms was assessed (Colony forming units (CFUs) and OD620nm readings). Overall, the strains with induced resistance to vancomycin formed biofilms with less biomass and CFUs than the isogenic strains. The acquisition of resistance to vancomycin in clinical strains of S. epidermidis seems to cause a decrease in the capacity of these strains to form biofilms. As such, in S. epidermidis, the resistance to vancomycin does not seem to be related to a stronger biofilm formation capacity.This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project EXPL/BIA-MIC/0032/2021.info:eu-repo/semantics/publishedVersionUniversidade da Beira Interior (UBI)Universidade do MinhoSantos, Inês OliveiraFrança, Ângela Maria Oliveira Sousa2023-12-072023-12-07T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/90895engSantos, Inês; França, Angela, Induced resistance to vancomycin impairs biofilm formation by S. epidermidis. Microbiotec23 - Congress of Microbiology and Biotechnology 2023. No. P4.65, Covilhã, Portugal, Dec 07-09, 450, 2023.https://microbiotec23.organideia.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:01:08Zoai:repositorium.sdum.uminho.pt:1822/90895Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:01:08Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis |
title |
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis |
spellingShingle |
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis Santos, Inês Oliveira Staphylococcus epidermidis Biofilms Antibiotic resistance Vancomycin |
title_short |
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis |
title_full |
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis |
title_fullStr |
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis |
title_full_unstemmed |
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis |
title_sort |
Induced resistance to vancomycin impairs biofilm formation by S. epidermidis |
author |
Santos, Inês Oliveira |
author_facet |
Santos, Inês Oliveira França, Ângela Maria Oliveira Sousa |
author_role |
author |
author2 |
França, Ângela Maria Oliveira Sousa |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, Inês Oliveira França, Ângela Maria Oliveira Sousa |
dc.subject.por.fl_str_mv |
Staphylococcus epidermidis Biofilms Antibiotic resistance Vancomycin |
topic |
Staphylococcus epidermidis Biofilms Antibiotic resistance Vancomycin |
description |
Coagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis, are one of the leading causes of sepsis in preterm neonates mostly due to their capacity to form biofilms on medical devices. A major concern regarding CoNS infections is associated with the increased antibiotic resistance that has been observed among this bacterial group. Hence, the treatment of these infections is becoming progressively challenging, which may lead to increased morbidity and mortality in preterm neonates. In that sense, we aim to explore the consequences of antibiotic resistance in biofilm formation by S. epidermidis. First, the susceptibility profile of S. epidermidis strains collected from Portuguese newborns with bloodstream infections was characterized. To induce resistance to vancomycin, the susceptible strains were exposed to increasing concentrations of the antibiotic and, after 36 passages, the MIC was re-evaluated and the capacity of vancomycin-induced resistance strains to form biofilms was assessed (Colony forming units (CFUs) and OD620nm readings). Overall, the strains with induced resistance to vancomycin formed biofilms with less biomass and CFUs than the isogenic strains. The acquisition of resistance to vancomycin in clinical strains of S. epidermidis seems to cause a decrease in the capacity of these strains to form biofilms. As such, in S. epidermidis, the resistance to vancomycin does not seem to be related to a stronger biofilm formation capacity. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-07 2023-12-07T00:00:00Z |
dc.type.driver.fl_str_mv |
conference object |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/90895 |
url |
https://hdl.handle.net/1822/90895 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, Inês; França, Angela, Induced resistance to vancomycin impairs biofilm formation by S. epidermidis. Microbiotec23 - Congress of Microbiology and Biotechnology 2023. No. P4.65, Covilhã, Portugal, Dec 07-09, 450, 2023. https://microbiotec23.organideia.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade da Beira Interior (UBI) |
publisher.none.fl_str_mv |
Universidade da Beira Interior (UBI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545173430697984 |