Induced resistance to vancomycin impairs biofilm formation by S. epidermidis

Detalhes bibliográficos
Autor(a) principal: Santos, Inês Oliveira
Data de Publicação: 2023
Outros Autores: França, Ângela Maria Oliveira Sousa
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/90895
Resumo: Coagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis, are one of the leading causes of sepsis in preterm neonates mostly due to their capacity to form biofilms on medical devices. A major concern regarding CoNS infections is associated with the increased antibiotic resistance that has been observed among this bacterial group. Hence, the treatment of these infections is becoming progressively challenging, which may lead to increased morbidity and mortality in preterm neonates. In that sense, we aim to explore the consequences of antibiotic resistance in biofilm formation by S. epidermidis. First, the susceptibility profile of S. epidermidis strains collected from Portuguese newborns with bloodstream infections was characterized. To induce resistance to vancomycin, the susceptible strains were exposed to increasing concentrations of the antibiotic and, after 36 passages, the MIC was re-evaluated and the capacity of vancomycin-induced resistance strains to form biofilms was assessed (Colony forming units (CFUs) and OD620nm readings). Overall, the strains with induced resistance to vancomycin formed biofilms with less biomass and CFUs than the isogenic strains. The acquisition of resistance to vancomycin in clinical strains of S. epidermidis seems to cause a decrease in the capacity of these strains to form biofilms. As such, in S. epidermidis, the resistance to vancomycin does not seem to be related to a stronger biofilm formation capacity.
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spelling Induced resistance to vancomycin impairs biofilm formation by S. epidermidisStaphylococcus epidermidisBiofilmsAntibiotic resistanceVancomycinCoagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis, are one of the leading causes of sepsis in preterm neonates mostly due to their capacity to form biofilms on medical devices. A major concern regarding CoNS infections is associated with the increased antibiotic resistance that has been observed among this bacterial group. Hence, the treatment of these infections is becoming progressively challenging, which may lead to increased morbidity and mortality in preterm neonates. In that sense, we aim to explore the consequences of antibiotic resistance in biofilm formation by S. epidermidis. First, the susceptibility profile of S. epidermidis strains collected from Portuguese newborns with bloodstream infections was characterized. To induce resistance to vancomycin, the susceptible strains were exposed to increasing concentrations of the antibiotic and, after 36 passages, the MIC was re-evaluated and the capacity of vancomycin-induced resistance strains to form biofilms was assessed (Colony forming units (CFUs) and OD620nm readings). Overall, the strains with induced resistance to vancomycin formed biofilms with less biomass and CFUs than the isogenic strains. The acquisition of resistance to vancomycin in clinical strains of S. epidermidis seems to cause a decrease in the capacity of these strains to form biofilms. As such, in S. epidermidis, the resistance to vancomycin does not seem to be related to a stronger biofilm formation capacity.This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project EXPL/BIA-MIC/0032/2021.info:eu-repo/semantics/publishedVersionUniversidade da Beira Interior (UBI)Universidade do MinhoSantos, Inês OliveiraFrança, Ângela Maria Oliveira Sousa2023-12-072023-12-07T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/90895engSantos, Inês; França, Angela, Induced resistance to vancomycin impairs biofilm formation by S. epidermidis. Microbiotec23 - Congress of Microbiology and Biotechnology 2023. No. P4.65, Covilhã, Portugal, Dec 07-09, 450, 2023.https://microbiotec23.organideia.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:01:08Zoai:repositorium.sdum.uminho.pt:1822/90895Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:01:08Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
title Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
spellingShingle Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
Santos, Inês Oliveira
Staphylococcus epidermidis
Biofilms
Antibiotic resistance
Vancomycin
title_short Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
title_full Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
title_fullStr Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
title_full_unstemmed Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
title_sort Induced resistance to vancomycin impairs biofilm formation by S. epidermidis
author Santos, Inês Oliveira
author_facet Santos, Inês Oliveira
França, Ângela Maria Oliveira Sousa
author_role author
author2 França, Ângela Maria Oliveira Sousa
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Inês Oliveira
França, Ângela Maria Oliveira Sousa
dc.subject.por.fl_str_mv Staphylococcus epidermidis
Biofilms
Antibiotic resistance
Vancomycin
topic Staphylococcus epidermidis
Biofilms
Antibiotic resistance
Vancomycin
description Coagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis, are one of the leading causes of sepsis in preterm neonates mostly due to their capacity to form biofilms on medical devices. A major concern regarding CoNS infections is associated with the increased antibiotic resistance that has been observed among this bacterial group. Hence, the treatment of these infections is becoming progressively challenging, which may lead to increased morbidity and mortality in preterm neonates. In that sense, we aim to explore the consequences of antibiotic resistance in biofilm formation by S. epidermidis. First, the susceptibility profile of S. epidermidis strains collected from Portuguese newborns with bloodstream infections was characterized. To induce resistance to vancomycin, the susceptible strains were exposed to increasing concentrations of the antibiotic and, after 36 passages, the MIC was re-evaluated and the capacity of vancomycin-induced resistance strains to form biofilms was assessed (Colony forming units (CFUs) and OD620nm readings). Overall, the strains with induced resistance to vancomycin formed biofilms with less biomass and CFUs than the isogenic strains. The acquisition of resistance to vancomycin in clinical strains of S. epidermidis seems to cause a decrease in the capacity of these strains to form biofilms. As such, in S. epidermidis, the resistance to vancomycin does not seem to be related to a stronger biofilm formation capacity.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-07
2023-12-07T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/90895
url https://hdl.handle.net/1822/90895
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos, Inês; França, Angela, Induced resistance to vancomycin impairs biofilm formation by S. epidermidis. Microbiotec23 - Congress of Microbiology and Biotechnology 2023. No. P4.65, Covilhã, Portugal, Dec 07-09, 450, 2023.
https://microbiotec23.organideia.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade da Beira Interior (UBI)
publisher.none.fl_str_mv Universidade da Beira Interior (UBI)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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