Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/3994 |
Resumo: | The mode of action of PTHrP in the regulation of sea bream (Sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system. Piscine (1–34)PTHrP (10−6–10−11 m) stimulated cortisol production in a dose-dependent manner. The ED50 of (1–34)PTHrP was 2.8 times higher than that of (1–39)ACTH, and maximum increase in cortisol production in response to 10−8 m of (1–34)PTHrP was approximately 7-fold lower than for 10−8 m of (1–39)ACTH. In contrast to (1–34)PTHrP, piscine (10–20)PTHrP, (79–93)PTHrP, and (100–125)PTHrP (10−9–10−7 m) did not stimulate cortisol production. The effect of piscine (1–34)PTHrP on cortisol production was abolished by N-terminal peptides in which the first amino acid (Ser) was absent and by simultaneous addition of inhibitors of the adenylyl cyclase-protein kinase A and phospholipase C-protein kinase C intracellular pathways but not by each separately. The PTHrP-induced signal transduction was further investigated by measurements of cAMP production and [H3]myo-inositol incorporation in an interrenal cell suspension. Piscine (1–34)PTHrP increased cAMP and total inositol phosphate accumulation, which is indicative that the mechanism of action of PTHrP in interrenal tissue involves the activation of both the adenylyl cyclase-cAMP and phospholipase C-inositol phosphate signaling pathways. These results, together with the expression of mRNA for PTHrP and for PTH receptor (PTHR) type 1 and PTHR type 3 receptors in sea bream interrenal tissue, suggest a specific paracrine or autocrine steroidogenic action of PTHrP mediated by the PTHRs. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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spelling |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitroThe mode of action of PTHrP in the regulation of sea bream (Sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system. Piscine (1–34)PTHrP (10−6–10−11 m) stimulated cortisol production in a dose-dependent manner. The ED50 of (1–34)PTHrP was 2.8 times higher than that of (1–39)ACTH, and maximum increase in cortisol production in response to 10−8 m of (1–34)PTHrP was approximately 7-fold lower than for 10−8 m of (1–39)ACTH. In contrast to (1–34)PTHrP, piscine (10–20)PTHrP, (79–93)PTHrP, and (100–125)PTHrP (10−9–10−7 m) did not stimulate cortisol production. The effect of piscine (1–34)PTHrP on cortisol production was abolished by N-terminal peptides in which the first amino acid (Ser) was absent and by simultaneous addition of inhibitors of the adenylyl cyclase-protein kinase A and phospholipase C-protein kinase C intracellular pathways but not by each separately. The PTHrP-induced signal transduction was further investigated by measurements of cAMP production and [H3]myo-inositol incorporation in an interrenal cell suspension. Piscine (1–34)PTHrP increased cAMP and total inositol phosphate accumulation, which is indicative that the mechanism of action of PTHrP in interrenal tissue involves the activation of both the adenylyl cyclase-cAMP and phospholipase C-inositol phosphate signaling pathways. These results, together with the expression of mRNA for PTHrP and for PTH receptor (PTHR) type 1 and PTHR type 3 receptors in sea bream interrenal tissue, suggest a specific paracrine or autocrine steroidogenic action of PTHrP mediated by the PTHRs.Endocrine SocietySapientiaRotllant, J.Guerreiro, P. M.Anjos, LilianaRedruello, BegoñaCanario, Adelino V. M.Power, Deborah2014-05-17T10:51:50Z20052014-05-13T10:08:55Z2005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/3994engRotllant, J.; Guerreiro, P. M.; Anjos, L.; Redruello, B.; Canario, A. V. M.; Power, D. M.Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro, Endocrinology, 146, 1, 71-76, 2005.0013-7227AUT: ACA00258; DPO00386;http://dx.doi.org/10.1210/en.2004-0644info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:15:05Zoai:sapientia.ualg.pt:10400.1/3994Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:57:25.430335Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro |
title |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro |
spellingShingle |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro Rotllant, J. |
title_short |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro |
title_full |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro |
title_fullStr |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro |
title_full_unstemmed |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro |
title_sort |
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro |
author |
Rotllant, J. |
author_facet |
Rotllant, J. Guerreiro, P. M. Anjos, Liliana Redruello, Begoña Canario, Adelino V. M. Power, Deborah |
author_role |
author |
author2 |
Guerreiro, P. M. Anjos, Liliana Redruello, Begoña Canario, Adelino V. M. Power, Deborah |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Rotllant, J. Guerreiro, P. M. Anjos, Liliana Redruello, Begoña Canario, Adelino V. M. Power, Deborah |
description |
The mode of action of PTHrP in the regulation of sea bream (Sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system. Piscine (1–34)PTHrP (10−6–10−11 m) stimulated cortisol production in a dose-dependent manner. The ED50 of (1–34)PTHrP was 2.8 times higher than that of (1–39)ACTH, and maximum increase in cortisol production in response to 10−8 m of (1–34)PTHrP was approximately 7-fold lower than for 10−8 m of (1–39)ACTH. In contrast to (1–34)PTHrP, piscine (10–20)PTHrP, (79–93)PTHrP, and (100–125)PTHrP (10−9–10−7 m) did not stimulate cortisol production. The effect of piscine (1–34)PTHrP on cortisol production was abolished by N-terminal peptides in which the first amino acid (Ser) was absent and by simultaneous addition of inhibitors of the adenylyl cyclase-protein kinase A and phospholipase C-protein kinase C intracellular pathways but not by each separately. The PTHrP-induced signal transduction was further investigated by measurements of cAMP production and [H3]myo-inositol incorporation in an interrenal cell suspension. Piscine (1–34)PTHrP increased cAMP and total inositol phosphate accumulation, which is indicative that the mechanism of action of PTHrP in interrenal tissue involves the activation of both the adenylyl cyclase-cAMP and phospholipase C-inositol phosphate signaling pathways. These results, together with the expression of mRNA for PTHrP and for PTH receptor (PTHR) type 1 and PTHR type 3 receptors in sea bream interrenal tissue, suggest a specific paracrine or autocrine steroidogenic action of PTHrP mediated by the PTHRs. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2005-01-01T00:00:00Z 2014-05-17T10:51:50Z 2014-05-13T10:08:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/3994 |
url |
http://hdl.handle.net/10400.1/3994 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rotllant, J.; Guerreiro, P. M.; Anjos, L.; Redruello, B.; Canario, A. V. M.; Power, D. M.Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro, Endocrinology, 146, 1, 71-76, 2005. 0013-7227 AUT: ACA00258; DPO00386; http://dx.doi.org/10.1210/en.2004-0644 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Endocrine Society |
publisher.none.fl_str_mv |
Endocrine Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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