Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.

Detalhes bibliográficos
Autor(a) principal: Sánchez-Andrea, Irene
Data de Publicação: 2022
Outros Autores: van der Graaf, Charlotte M., Hornung, Bastian, Bale, Nicole J., Jarzembowska, Monika, Sousa, Diana Zita Machado, Rijpstra, W. Irene C., Sinninghe Damsté, Jaap S., Stams, Alfons Johannes Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/77251
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2022.816605/full#supplementary-material
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spelling Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.acid rock/mine drainageacidophilessulfate-reducing bacteriaAcididesulfobacillusDesulfosporosinusDesulfitobacteriumacetate oxidationbioremediationacid rockmine drainageScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2022.816605/full#supplementary-materialIn acid drainage environments, biosulfidogenesis by sulfate-reducing bacteria (SRB) attenuates the extreme conditions by enabling the precipitation of metals as their sulfides, and the neutralization of acidity through proton consumption. So far, only a handful of moderately acidophilic SRB species have been described, most of which are merely acidotolerant. Here, a novel species within a novel genus of moderately acidophilic SRB is described, Acididesulfobacillus acetoxydans gen. nov. sp. nov. strain INE, able to grow at pH 3.8. Bioreactor studies with strain INE at optimum (5.0) and low (3.9) pH for growth showed that strain INE alkalinized its environment, and that this was more pronounced at lower pH. These studies also showed the capacity of strain INE to completely oxidize organic acids to CO2, which is uncommon among acidophilic SRB. Since organic acids are mainly in their protonated form at low pH, which increases their toxicity, their complete oxidation may be an acid stress resistance mechanism. Comparative proteogenomic and membrane lipid analysis further indicated that the presence of saturated ether-bound lipids in the membrane, and their relative increase at lower pH, was a protection mechanism against acid stress. Interestingly, other canonical acid stress resistance mechanisms, such as a Donnan potential and increased active charge transport, did not appear to be active.This work was financed by ERC grants to AS (project 323009) and JS (project 694569), the research program TTW under project number 14797, which is financed by the Dutch Research Council (NWO) to IS-A, and a Gravitation grant (SIAM 024.002.002) of the Netherlands Ministry of Education, Culture and Science to AS and JS.info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoSánchez-Andrea, Irenevan der Graaf, Charlotte M.Hornung, BastianBale, Nicole J.Jarzembowska, MonikaSousa, Diana Zita MachadoRijpstra, W. Irene C.Sinninghe Damsté, Jaap S.Stams, Alfons Johannes Maria2022-03-042022-03-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/77251engSánchez-Andrea, Irene; van der Graaf, Charlotte M.; Hornung, Bastian; Bale, Nicole J.; Jarzembowska, Monika; Sousa, Diana Z.; Rijpstra, W. Irene C.; Sinninghe Damsté, Jaap S.; Stams, A. J. M., Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.. Frontiers in Microbiology, 13(816605), 20221664-302X10.3389/fmicb.2022.816605816605http://journal.frontiersin.org/journal/microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:59Zoai:repositorium.sdum.uminho.pt:1822/77251Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:58.578836Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.
title Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.
spellingShingle Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.
Sánchez-Andrea, Irene
acid rock/mine drainage
acidophiles
sulfate-reducing bacteria
Acididesulfobacillus
Desulfosporosinus
Desulfitobacterium
acetate oxidation
bioremediation
acid rock
mine drainage
Science & Technology
title_short Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.
title_full Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.
title_fullStr Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.
title_full_unstemmed Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.
title_sort Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.
author Sánchez-Andrea, Irene
author_facet Sánchez-Andrea, Irene
van der Graaf, Charlotte M.
Hornung, Bastian
Bale, Nicole J.
Jarzembowska, Monika
Sousa, Diana Zita Machado
Rijpstra, W. Irene C.
Sinninghe Damsté, Jaap S.
Stams, Alfons Johannes Maria
author_role author
author2 van der Graaf, Charlotte M.
Hornung, Bastian
Bale, Nicole J.
Jarzembowska, Monika
Sousa, Diana Zita Machado
Rijpstra, W. Irene C.
Sinninghe Damsté, Jaap S.
Stams, Alfons Johannes Maria
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sánchez-Andrea, Irene
van der Graaf, Charlotte M.
Hornung, Bastian
Bale, Nicole J.
Jarzembowska, Monika
Sousa, Diana Zita Machado
Rijpstra, W. Irene C.
Sinninghe Damsté, Jaap S.
Stams, Alfons Johannes Maria
dc.subject.por.fl_str_mv acid rock/mine drainage
acidophiles
sulfate-reducing bacteria
Acididesulfobacillus
Desulfosporosinus
Desulfitobacterium
acetate oxidation
bioremediation
acid rock
mine drainage
Science & Technology
topic acid rock/mine drainage
acidophiles
sulfate-reducing bacteria
Acididesulfobacillus
Desulfosporosinus
Desulfitobacterium
acetate oxidation
bioremediation
acid rock
mine drainage
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2022.816605/full#supplementary-material
publishDate 2022
dc.date.none.fl_str_mv 2022-03-04
2022-03-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/77251
url http://hdl.handle.net/1822/77251
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sánchez-Andrea, Irene; van der Graaf, Charlotte M.; Hornung, Bastian; Bale, Nicole J.; Jarzembowska, Monika; Sousa, Diana Z.; Rijpstra, W. Irene C.; Sinninghe Damsté, Jaap S.; Stams, A. J. M., Acetate degradation at low pH by the moderately acidophilic sulfate reducer Acididesulfobacillus acetoxydans gen. nov. sp. nov.. Frontiers in Microbiology, 13(816605), 2022
1664-302X
10.3389/fmicb.2022.816605
816605
http://journal.frontiersin.org/journal/microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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