A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles

Detalhes bibliográficos
Autor(a) principal: Rosa Pereira
Data de Publicação: 2010
Outros Autores: Tommy Julianto, Peh Kim Ang, Sharon Sheue Nee Ling, Carlos Mauricio Barbosa, Kah Hay Yuen, Abu Bakar Abdul Majeed
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/81233
Resumo: A sensitive and rapid routine LC method was validated for measuring cefotaxime incorporated in three different pH-sensitive nanoparticles. The drug was chromatographed on a C18 reversed-phase column; the mobile phase used was 0.05 M aqueous ammonium acetate, acetonitrile and tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5 with acetic acid. The flow rate was 1 mL min(-1) and cefotaxime was quantified at 254 nm, with a sensitivity range of 0.005 AUFS. The validated method was specific, linear (R (2) a parts per thousand yen 0.999), precise and accurate in a concentration range of 0.2-50.0 mu g mL(-1). The method was rapid, selective and suitable for evaluation of cefotaxime in pH-sensitive Eudragit nanoparticles.
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spelling A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive NanoparticlesQuímicaChemical sciencesA sensitive and rapid routine LC method was validated for measuring cefotaxime incorporated in three different pH-sensitive nanoparticles. The drug was chromatographed on a C18 reversed-phase column; the mobile phase used was 0.05 M aqueous ammonium acetate, acetonitrile and tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5 with acetic acid. The flow rate was 1 mL min(-1) and cefotaxime was quantified at 254 nm, with a sensitivity range of 0.005 AUFS. The validated method was specific, linear (R (2) a parts per thousand yen 0.999), precise and accurate in a concentration range of 0.2-50.0 mu g mL(-1). The method was rapid, selective and suitable for evaluation of cefotaxime in pH-sensitive Eudragit nanoparticles.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/81233eng0009-589310.1365/s10337-010-1481-5Rosa PereiraTommy JuliantoPeh Kim AngSharon Sheue Nee LingCarlos Mauricio BarbosaKah Hay YuenAbu Bakar Abdul Majeedinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:58:33Zoai:repositorio-aberto.up.pt:10216/81233Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:51:20.407812Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles
title A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles
spellingShingle A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles
Rosa Pereira
Química
Chemical sciences
title_short A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles
title_full A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles
title_fullStr A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles
title_full_unstemmed A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles
title_sort A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles
author Rosa Pereira
author_facet Rosa Pereira
Tommy Julianto
Peh Kim Ang
Sharon Sheue Nee Ling
Carlos Mauricio Barbosa
Kah Hay Yuen
Abu Bakar Abdul Majeed
author_role author
author2 Tommy Julianto
Peh Kim Ang
Sharon Sheue Nee Ling
Carlos Mauricio Barbosa
Kah Hay Yuen
Abu Bakar Abdul Majeed
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rosa Pereira
Tommy Julianto
Peh Kim Ang
Sharon Sheue Nee Ling
Carlos Mauricio Barbosa
Kah Hay Yuen
Abu Bakar Abdul Majeed
dc.subject.por.fl_str_mv Química
Chemical sciences
topic Química
Chemical sciences
description A sensitive and rapid routine LC method was validated for measuring cefotaxime incorporated in three different pH-sensitive nanoparticles. The drug was chromatographed on a C18 reversed-phase column; the mobile phase used was 0.05 M aqueous ammonium acetate, acetonitrile and tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5 with acetic acid. The flow rate was 1 mL min(-1) and cefotaxime was quantified at 254 nm, with a sensitivity range of 0.005 AUFS. The validated method was specific, linear (R (2) a parts per thousand yen 0.999), precise and accurate in a concentration range of 0.2-50.0 mu g mL(-1). The method was rapid, selective and suitable for evaluation of cefotaxime in pH-sensitive Eudragit nanoparticles.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/81233
url https://hdl.handle.net/10216/81233
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0009-5893
10.1365/s10337-010-1481-5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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