Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.26/44975 https://doi.org/Liliana Truta, André L. Castro, Sónia Tarelho, Pedro Costa, M. Goreti F. Sales, Helena M. Teixeira, Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes, Journal of Pharmaceutical and Biomedical Analysis, Volume 128, 2016, Pages 496-503, https://doi.org/10.1016/j.jpba.2016.06.027. https://doi.org/10.1016/j.jpba.2016.06.027 |
Resumo: | Depression is among the most prevalent psychiatric disorders of our society, leading to an increase in antidepressant drug consumption that needs to be accurately determined in whole blood samples in Forensic Toxicology Laboratories. For this purpose, this work presents a new gas chromatography tandem mass spectrometry (GC–MS/MS) method targeting the simultaneous and rapid determination of 14 common Antidepressants in whole blood: 13 Antidepressants (amitriptyline, citalopram, clomipramine, dothiepin, fluoxetine, imipramine, mianserin, mirtazapine, nortryptiline, paroxetine, sertraline, trimipramine and venlafaxine) and 1 Metabolite (N-desmethylclomipramine). Solid-phase extraction was used prior to chromatographic separation. Chromatographic and MS/MS parameters were selected to improve sensitivity, peak resolution and unequivocal identification of the eluted analyte. The detection was performed on a triple quadrupole tandem MS in selected ion monitoring (SIM) mode in tandem, using electronic impact ionization. Clomipramine-D3 and trimipramine-D3 were used as deutered internal standards. The validation parameters included linearity, limits of detection, lower limit of quantification, selectivity/ specificity, extraction efficiency, carry-over, precision and robustness, and followed internationally accepted guidelines. Limits of quantification and detection were lower than therapeutic and subtherapeutic concentration ranges. Overall, the method offered good selectivity, robustness and quick response (<16 min) for typical concentration ranges, both for therapeutic and lethal levels. |
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Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposesAntidepressantsGC–MS/MSWhole bloodSolid-phase extractionForensic toxicologyDepression is among the most prevalent psychiatric disorders of our society, leading to an increase in antidepressant drug consumption that needs to be accurately determined in whole blood samples in Forensic Toxicology Laboratories. For this purpose, this work presents a new gas chromatography tandem mass spectrometry (GC–MS/MS) method targeting the simultaneous and rapid determination of 14 common Antidepressants in whole blood: 13 Antidepressants (amitriptyline, citalopram, clomipramine, dothiepin, fluoxetine, imipramine, mianserin, mirtazapine, nortryptiline, paroxetine, sertraline, trimipramine and venlafaxine) and 1 Metabolite (N-desmethylclomipramine). Solid-phase extraction was used prior to chromatographic separation. Chromatographic and MS/MS parameters were selected to improve sensitivity, peak resolution and unequivocal identification of the eluted analyte. The detection was performed on a triple quadrupole tandem MS in selected ion monitoring (SIM) mode in tandem, using electronic impact ionization. Clomipramine-D3 and trimipramine-D3 were used as deutered internal standards. The validation parameters included linearity, limits of detection, lower limit of quantification, selectivity/ specificity, extraction efficiency, carry-over, precision and robustness, and followed internationally accepted guidelines. Limits of quantification and detection were lower than therapeutic and subtherapeutic concentration ranges. Overall, the method offered good selectivity, robustness and quick response (<16 min) for typical concentration ranges, both for therapeutic and lethal levels.info:eu-repo/semantics/acceptedVersionElsevier2023-05-30T15:38:22Z2023-05-302016-06-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10400.26/44975https://doi.org/Liliana Truta, André L. Castro, Sónia Tarelho, Pedro Costa, M. Goreti F. Sales, Helena M. Teixeira, Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes, Journal of Pharmaceutical and Biomedical Analysis, Volume 128, 2016, Pages 496-503, https://doi.org/10.1016/j.jpba.2016.06.027.http://hdl.handle.net/10400.26/44975https://doi.org/10.1016/j.jpba.2016.06.027enghttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTruta, LilianaCastro, André LoboTarelho, SóniaCosta, PedroSales, M. Goreti F.Teixeira, Helena M.reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-03T04:22:32Zoai:comum.rcaap.pt:10400.26/44975Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:07:50.101010Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes |
title |
Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes |
spellingShingle |
Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes Truta, Liliana Antidepressants GC–MS/MS Whole blood Solid-phase extraction Forensic toxicology |
title_short |
Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes |
title_full |
Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes |
title_fullStr |
Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes |
title_full_unstemmed |
Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes |
title_sort |
Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes |
author |
Truta, Liliana |
author_facet |
Truta, Liliana Castro, André Lobo Tarelho, Sónia Costa, Pedro Sales, M. Goreti F. Teixeira, Helena M. |
author_role |
author |
author2 |
Castro, André Lobo Tarelho, Sónia Costa, Pedro Sales, M. Goreti F. Teixeira, Helena M. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Truta, Liliana Castro, André Lobo Tarelho, Sónia Costa, Pedro Sales, M. Goreti F. Teixeira, Helena M. |
dc.subject.por.fl_str_mv |
Antidepressants GC–MS/MS Whole blood Solid-phase extraction Forensic toxicology |
topic |
Antidepressants GC–MS/MS Whole blood Solid-phase extraction Forensic toxicology |
description |
Depression is among the most prevalent psychiatric disorders of our society, leading to an increase in antidepressant drug consumption that needs to be accurately determined in whole blood samples in Forensic Toxicology Laboratories. For this purpose, this work presents a new gas chromatography tandem mass spectrometry (GC–MS/MS) method targeting the simultaneous and rapid determination of 14 common Antidepressants in whole blood: 13 Antidepressants (amitriptyline, citalopram, clomipramine, dothiepin, fluoxetine, imipramine, mianserin, mirtazapine, nortryptiline, paroxetine, sertraline, trimipramine and venlafaxine) and 1 Metabolite (N-desmethylclomipramine). Solid-phase extraction was used prior to chromatographic separation. Chromatographic and MS/MS parameters were selected to improve sensitivity, peak resolution and unequivocal identification of the eluted analyte. The detection was performed on a triple quadrupole tandem MS in selected ion monitoring (SIM) mode in tandem, using electronic impact ionization. Clomipramine-D3 and trimipramine-D3 were used as deutered internal standards. The validation parameters included linearity, limits of detection, lower limit of quantification, selectivity/ specificity, extraction efficiency, carry-over, precision and robustness, and followed internationally accepted guidelines. Limits of quantification and detection were lower than therapeutic and subtherapeutic concentration ranges. Overall, the method offered good selectivity, robustness and quick response (<16 min) for typical concentration ranges, both for therapeutic and lethal levels. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-23T00:00:00Z 2023-05-30T15:38:22Z 2023-05-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.26/44975 https://doi.org/Liliana Truta, André L. Castro, Sónia Tarelho, Pedro Costa, M. Goreti F. Sales, Helena M. Teixeira, Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes, Journal of Pharmaceutical and Biomedical Analysis, Volume 128, 2016, Pages 496-503, https://doi.org/10.1016/j.jpba.2016.06.027. http://hdl.handle.net/10400.26/44975 https://doi.org/10.1016/j.jpba.2016.06.027 |
url |
http://hdl.handle.net/10400.26/44975 https://doi.org/Liliana Truta, André L. Castro, Sónia Tarelho, Pedro Costa, M. Goreti F. Sales, Helena M. Teixeira, Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes, Journal of Pharmaceutical and Biomedical Analysis, Volume 128, 2016, Pages 496-503, https://doi.org/10.1016/j.jpba.2016.06.027. https://doi.org/10.1016/j.jpba.2016.06.027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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