Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice

Detalhes bibliográficos
Autor(a) principal: Queiroga, Cláudia S F
Data de Publicação: 2016
Outros Autores: Alves, Raquel M A, Conde, Sílvia V, Alves, Paula M, Vieira, Helena L A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/21942
Resumo: © 2016. Published by The Company of Biologists Ltd.
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spelling Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in miceApoptosisBrainCarbon monoxideCo-culturesPurinergicPreconditioning© 2016. Published by The Company of Biologists Ltd.The neuroprotective role of carbon monoxide (CO) has been studied in a cell-autonomous mode. Herein, a new concept is disclosed - CO affects astrocyte-neuron communication in a paracrine manner to promote neuroprotection. Neuronal survival was assessed when co-cultured with astrocytes that had been pre-treated or not with CO. The CO-pre-treated astrocytes reduced neuronal cell death, and the cellular mechanisms were investigated, focusing on purinergic signaling. CO modulates astrocytic metabolism and extracellular ATP content in the co-culture medium. Moreover, several antagonists of P1 adenosine and P2 ATP receptors partially reverted CO-induced neuroprotection through astrocytes. Likewise, knocking down expression of the neuronal P1 adenosine receptor A2A-R (encoded by Adora2a) reverted the neuroprotective effects of CO-exposed astrocytes. The neuroprotection of CO-treated astrocytes also decreased following prevention of ATP or adenosine release from astrocytic cells and inhibition of extracellular ATP metabolism into adenosine. Finally, the neuronal downstream event involves TrkB (also known as NTRK2) receptors and BDNF. Pharmacological and genetic inhibition of TrkB receptors reverts neuroprotection triggered by CO-treated astrocytes. Furthermore, the neuronal ratio of BDNF to pro-BDNF increased in the presence of CO-treated astrocytes and decreased whenever A2A-R expression was silenced. In summary, CO prevents neuronal cell death in a paracrine manner by targeting astrocytic metabolism through purinergic signaling.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNQueiroga, Cláudia S FAlves, Raquel M AConde, Sílvia VAlves, Paula MVieira, Helena L A2017-07-13T22:01:16Z2016-08-152016-08-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/21942eng0021-9533PURE: 1755286https://doi.org/10.1242/jcs.187260info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:09:06Zoai:run.unl.pt:10362/21942Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:03.237223Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice
title Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice
spellingShingle Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice
Queiroga, Cláudia S F
Apoptosis
Brain
Carbon monoxide
Co-cultures
Purinergic
Preconditioning
title_short Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice
title_full Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice
title_fullStr Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice
title_full_unstemmed Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice
title_sort Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice
author Queiroga, Cláudia S F
author_facet Queiroga, Cláudia S F
Alves, Raquel M A
Conde, Sílvia V
Alves, Paula M
Vieira, Helena L A
author_role author
author2 Alves, Raquel M A
Conde, Sílvia V
Alves, Paula M
Vieira, Helena L A
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Queiroga, Cláudia S F
Alves, Raquel M A
Conde, Sílvia V
Alves, Paula M
Vieira, Helena L A
dc.subject.por.fl_str_mv Apoptosis
Brain
Carbon monoxide
Co-cultures
Purinergic
Preconditioning
topic Apoptosis
Brain
Carbon monoxide
Co-cultures
Purinergic
Preconditioning
description © 2016. Published by The Company of Biologists Ltd.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-15
2016-08-15T00:00:00Z
2017-07-13T22:01:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/21942
url http://hdl.handle.net/10362/21942
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9533
PURE: 1755286
https://doi.org/10.1242/jcs.187260
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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