The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/51165 |
Resumo: | Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment. |
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The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathwaysScience & TechnologyMethanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.Research was funded by grants of the Division of Chemical Sciences (CW-TOP 700.55.343) and Earth and Life Sciences (ALW 819.02.014) of The Netherlands Organisation for Scientific Research (NWO), the European Research Council (ERC grant 323009), and the Gravitation grant (024.002.002) of the Netherlands Ministry of Education, Culture and Scienceinfo:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoSousa, Diana Zita MachadoVisser, Michaelvan Gelder, Antonie H.Boeren, SjefPieterse, Mervin M.Pinkse, Martijn W. H.Verhaert, Peter D. E. M.Vogt, CarstenFranke, SteffiKümmel,SteffenStams, Alfons Johannes Maria2018-01-182018-01-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51165engSousa, Diana Z.; Visser, Michael; van Gelder, Antonie H.; Boeren, Sjef; Pieterse, Mervin M.; Pinkse, Martijn W. H.; Verhaert, Peter D. E. M.; Vogt, Carsten; Franke, Steffi; Kümmel,Steffen; Stams, A. J. M., The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways. Nature Communications, 9(239), 20182041-17232041-172310.1038/s41467-017-02518-929339722https://www.nature.com/ncomms/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:04Zoai:repositorium.sdum.uminho.pt:1822/51165Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:41.801295Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways |
title |
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways |
spellingShingle |
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways Sousa, Diana Zita Machado Science & Technology |
title_short |
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways |
title_full |
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways |
title_fullStr |
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways |
title_full_unstemmed |
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways |
title_sort |
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways |
author |
Sousa, Diana Zita Machado |
author_facet |
Sousa, Diana Zita Machado Visser, Michael van Gelder, Antonie H. Boeren, Sjef Pieterse, Mervin M. Pinkse, Martijn W. H. Verhaert, Peter D. E. M. Vogt, Carsten Franke, Steffi Kümmel,Steffen Stams, Alfons Johannes Maria |
author_role |
author |
author2 |
Visser, Michael van Gelder, Antonie H. Boeren, Sjef Pieterse, Mervin M. Pinkse, Martijn W. H. Verhaert, Peter D. E. M. Vogt, Carsten Franke, Steffi Kümmel,Steffen Stams, Alfons Johannes Maria |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sousa, Diana Zita Machado Visser, Michael van Gelder, Antonie H. Boeren, Sjef Pieterse, Mervin M. Pinkse, Martijn W. H. Verhaert, Peter D. E. M. Vogt, Carsten Franke, Steffi Kümmel,Steffen Stams, Alfons Johannes Maria |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-18 2018-01-18T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/51165 |
url |
http://hdl.handle.net/1822/51165 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sousa, Diana Z.; Visser, Michael; van Gelder, Antonie H.; Boeren, Sjef; Pieterse, Mervin M.; Pinkse, Martijn W. H.; Verhaert, Peter D. E. M.; Vogt, Carsten; Franke, Steffi; Kümmel,Steffen; Stams, A. J. M., The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways. Nature Communications, 9(239), 2018 2041-1723 2041-1723 10.1038/s41467-017-02518-9 29339722 https://www.nature.com/ncomms/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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