Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development

Detalhes bibliográficos
Autor(a) principal: Furtado, João
Data de Publicação: 2014
Outros Autores: Bento, Margaret, Correia, Elizabeth, Inácio, José Manuel, Belo, José António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/22417
Resumo: J. Furtado is a PhD student funded by FCT (SFRH/BD/65628/2009). M. Bento (SFRH/BPD/86497/2012) and J. M. Inacio (SFRH/BPD/41081/2007) are Fundacao Ciencia e Tecnologia (FCT) pos-doctoral fellows. E. Correia is a recipient of a F.C.T.B.I. fellowship (PTDC/SAU-BID/114902/ 2009). This work was supported by research grants from FCT to J. A. Belo and from Institute for Biotechnology Bioengineering (Centro Biomedicina Molecular e Celular (IBB/CBME), Laboratorio Associado (LA) in the frame of Project Ref o PestOE/EQB/LA0023/2013.
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spelling Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart DevelopmentTRANSCRIPTION FACTORMIGRATIONTUBE FORMATIONAVIAN PRECARDIAC CELLSEXTRACELLULAR-MATRIXVENTRAL MORPHOGENESISMOVEMENT PATTERNSCARDIAC PROGENITOR CELLSNEURAL CRESTCONDUCTION SYSTEMCARDIAC PROGENITOR CELLSAVIAN PRECARDIAC CELLSNEURAL CRESTEXTRACELLULAR-MATRIXTRANSCRIPTION FACTORVENTRAL MORPHOGENESISMOVEMENT PATTERNSCONDUCTION SYSTEMTUBE FORMATIONMIGRATIONJ. Furtado is a PhD student funded by FCT (SFRH/BD/65628/2009). M. Bento (SFRH/BPD/86497/2012) and J. M. Inacio (SFRH/BPD/41081/2007) are Fundacao Ciencia e Tecnologia (FCT) pos-doctoral fellows. E. Correia is a recipient of a F.C.T.B.I. fellowship (PTDC/SAU-BID/114902/ 2009). This work was supported by research grants from FCT to J. A. Belo and from Institute for Biotechnology Bioengineering (Centro Biomedicina Molecular e Celular (IBB/CBME), Laboratorio Associado (LA) in the frame of Project Ref o PestOE/EQB/LA0023/2013.During the course of a differential screen to identify transcripts specific for chick heart/hemangioblast precursor cells, we have identified Ccbe1 (Collagen and calcium-binding EGF-like domain 1). While the importance of Ccbe1 for the development of the lymphatic system is now well demonstrated, its role in cardiac formation remained unknown. Here we show by whole-mount in situ hybridization analysis that cCcbe1 mRNA is initially detected in early cardiac progenitors of the two bilateral cardiogenic fields (HH4), and at later stages on the second heart field (HH9-18). Furthermore, cCcbe1 is expressed in multipotent and highly proliferative cardiac progenitors. We characterized the role of cCcbe1 during early cardiogenesis by performing functional studies. Upon morpholino-induced cCcbe1 knockdown, the chick embryos displayed heart malformations, which include aberrant fusion of the heart fields, leading to incomplete terminal differentiation of the cardiomyocytes. cCcbe1 overexpression also resulted in severe heart defects, including cardia bifida. Altogether, our data demonstrate that although cardiac progenitors cells are specified in cCcbe1 morphants, the migration and proliferation of cardiac precursors cells are impaired, suggesting that cCcbe1 is a key gene during early heart development.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNFurtado, JoãoBento, MargaretCorreia, ElizabethInácio, José ManuelBelo, José António2017-08-02T22:00:56Z2014-12-292014-12-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/22417eng1932-6203PURE: 319940https://doi.org/10.1371/journal.pone.0115481info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:09:55Zoai:run.unl.pt:10362/22417Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:17.303521Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
title Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
spellingShingle Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
Furtado, João
TRANSCRIPTION FACTOR
MIGRATION
TUBE FORMATION
AVIAN PRECARDIAC CELLS
EXTRACELLULAR-MATRIX
VENTRAL MORPHOGENESIS
MOVEMENT PATTERNS
CARDIAC PROGENITOR CELLS
NEURAL CREST
CONDUCTION SYSTEM
CARDIAC PROGENITOR CELLS
AVIAN PRECARDIAC CELLS
NEURAL CREST
EXTRACELLULAR-MATRIX
TRANSCRIPTION FACTOR
VENTRAL MORPHOGENESIS
MOVEMENT PATTERNS
CONDUCTION SYSTEM
TUBE FORMATION
MIGRATION
title_short Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
title_full Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
title_fullStr Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
title_full_unstemmed Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
title_sort Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
author Furtado, João
author_facet Furtado, João
Bento, Margaret
Correia, Elizabeth
Inácio, José Manuel
Belo, José António
author_role author
author2 Bento, Margaret
Correia, Elizabeth
Inácio, José Manuel
Belo, José António
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Furtado, João
Bento, Margaret
Correia, Elizabeth
Inácio, José Manuel
Belo, José António
dc.subject.por.fl_str_mv TRANSCRIPTION FACTOR
MIGRATION
TUBE FORMATION
AVIAN PRECARDIAC CELLS
EXTRACELLULAR-MATRIX
VENTRAL MORPHOGENESIS
MOVEMENT PATTERNS
CARDIAC PROGENITOR CELLS
NEURAL CREST
CONDUCTION SYSTEM
CARDIAC PROGENITOR CELLS
AVIAN PRECARDIAC CELLS
NEURAL CREST
EXTRACELLULAR-MATRIX
TRANSCRIPTION FACTOR
VENTRAL MORPHOGENESIS
MOVEMENT PATTERNS
CONDUCTION SYSTEM
TUBE FORMATION
MIGRATION
topic TRANSCRIPTION FACTOR
MIGRATION
TUBE FORMATION
AVIAN PRECARDIAC CELLS
EXTRACELLULAR-MATRIX
VENTRAL MORPHOGENESIS
MOVEMENT PATTERNS
CARDIAC PROGENITOR CELLS
NEURAL CREST
CONDUCTION SYSTEM
CARDIAC PROGENITOR CELLS
AVIAN PRECARDIAC CELLS
NEURAL CREST
EXTRACELLULAR-MATRIX
TRANSCRIPTION FACTOR
VENTRAL MORPHOGENESIS
MOVEMENT PATTERNS
CONDUCTION SYSTEM
TUBE FORMATION
MIGRATION
description J. Furtado is a PhD student funded by FCT (SFRH/BD/65628/2009). M. Bento (SFRH/BPD/86497/2012) and J. M. Inacio (SFRH/BPD/41081/2007) are Fundacao Ciencia e Tecnologia (FCT) pos-doctoral fellows. E. Correia is a recipient of a F.C.T.B.I. fellowship (PTDC/SAU-BID/114902/ 2009). This work was supported by research grants from FCT to J. A. Belo and from Institute for Biotechnology Bioengineering (Centro Biomedicina Molecular e Celular (IBB/CBME), Laboratorio Associado (LA) in the frame of Project Ref o PestOE/EQB/LA0023/2013.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-29
2014-12-29T00:00:00Z
2017-08-02T22:00:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/22417
url http://hdl.handle.net/10362/22417
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 319940
https://doi.org/10.1371/journal.pone.0115481
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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