Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/120352 |
Resumo: | Aptasensors have a great potential for environmental monitoring, particularly for real-time on-site detection of aquatic toxins produced by marine and freshwater microorganisms (cyanobacteria, dinoflagellates, and diatoms), with several advantages over other biosensors that are worth considering. Freshwater monitoring is of vital importance for public health, in numerous human activities, and animal welfare, since these toxins may cause fatal intoxications. Similarly, in marine waters, very effective monitoring programs have been put in place in many countries to detect when toxins exceed established regulatory levels and accordingly enforce shellfish harvesting closures. Recent advances in the fields of aptamer selection, nanomaterials and communication technologies, offer a vast array of possibilities to develop new imaginative strategies to create improved, ultrasensitive, reliable and real-time devices, featuring unique characteristics to produce and amplify the signal. So far, not many strategies have been used to detect aquatic toxins, mostly limited to the optic and electrochemical sensors, the majority applied to detect microcystin-LR using a target-induced switching mode. The limits of detection of these aptasensors have been decreasing from the nM to the fM order of magnitude in the past 20 years. Aspects related to sensor components, performance, aptamers sequences, matrices analyzed and future perspectives, are considered and discussed. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
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Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxinsBiosensorsClimate changeElectrochemical sensorsEnvironmental engineeringMetabolitesWaterAptasensorsEmerging toxinsEnvironmental MonitoringHarmful algal bloomsWater safetyToxic materialsaptamerfresh watermarine toxinsea wateranimalaquatic specieschemistrycyanobacteriumdiatomdinoflagellateenvironmental monitoringgenetic procedureshumanproceduresAnimalsAptamers, NucleotideAquatic OrganismsBiosensing TechniquesCyanobacteriaDiatomsDinoflagellidaEnvironmental MonitoringFresh WaterHumansMarine ToxinsSeawaterAptasensors have a great potential for environmental monitoring, particularly for real-time on-site detection of aquatic toxins produced by marine and freshwater microorganisms (cyanobacteria, dinoflagellates, and diatoms), with several advantages over other biosensors that are worth considering. Freshwater monitoring is of vital importance for public health, in numerous human activities, and animal welfare, since these toxins may cause fatal intoxications. Similarly, in marine waters, very effective monitoring programs have been put in place in many countries to detect when toxins exceed established regulatory levels and accordingly enforce shellfish harvesting closures. Recent advances in the fields of aptamer selection, nanomaterials and communication technologies, offer a vast array of possibilities to develop new imaginative strategies to create improved, ultrasensitive, reliable and real-time devices, featuring unique characteristics to produce and amplify the signal. So far, not many strategies have been used to detect aquatic toxins, mostly limited to the optic and electrochemical sensors, the majority applied to detect microcystin-LR using a target-induced switching mode. The limits of detection of these aptasensors have been decreasing from the nM to the fM order of magnitude in the past 20 years. Aspects related to sensor components, performance, aptamers sequences, matrices analyzed and future perspectives, are considered and discussed. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.MDPI20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120352eng14243210, 1424822010.3390/s18072367Cunha I.Biltes R.Sales M.G.F.Vasconcelos V.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:26:45Zoai:repositorio-aberto.up.pt:10216/120352Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:23:55.285588Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins |
title |
Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins |
spellingShingle |
Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins Cunha I. Biosensors Climate change Electrochemical sensors Environmental engineering Metabolites Water Aptasensors Emerging toxins Environmental Monitoring Harmful algal blooms Water safety Toxic materials aptamer fresh water marine toxin sea water animal aquatic species chemistry cyanobacterium diatom dinoflagellate environmental monitoring genetic procedures human procedures Animals Aptamers, Nucleotide Aquatic Organisms Biosensing Techniques Cyanobacteria Diatoms Dinoflagellida Environmental Monitoring Fresh Water Humans Marine Toxins Seawater |
title_short |
Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins |
title_full |
Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins |
title_fullStr |
Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins |
title_full_unstemmed |
Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins |
title_sort |
Aptamer-based biosensors to detect aquatic phycotoxins and cyanotoxins |
author |
Cunha I. |
author_facet |
Cunha I. Biltes R. Sales M.G.F. Vasconcelos V. |
author_role |
author |
author2 |
Biltes R. Sales M.G.F. Vasconcelos V. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cunha I. Biltes R. Sales M.G.F. Vasconcelos V. |
dc.subject.por.fl_str_mv |
Biosensors Climate change Electrochemical sensors Environmental engineering Metabolites Water Aptasensors Emerging toxins Environmental Monitoring Harmful algal blooms Water safety Toxic materials aptamer fresh water marine toxin sea water animal aquatic species chemistry cyanobacterium diatom dinoflagellate environmental monitoring genetic procedures human procedures Animals Aptamers, Nucleotide Aquatic Organisms Biosensing Techniques Cyanobacteria Diatoms Dinoflagellida Environmental Monitoring Fresh Water Humans Marine Toxins Seawater |
topic |
Biosensors Climate change Electrochemical sensors Environmental engineering Metabolites Water Aptasensors Emerging toxins Environmental Monitoring Harmful algal blooms Water safety Toxic materials aptamer fresh water marine toxin sea water animal aquatic species chemistry cyanobacterium diatom dinoflagellate environmental monitoring genetic procedures human procedures Animals Aptamers, Nucleotide Aquatic Organisms Biosensing Techniques Cyanobacteria Diatoms Dinoflagellida Environmental Monitoring Fresh Water Humans Marine Toxins Seawater |
description |
Aptasensors have a great potential for environmental monitoring, particularly for real-time on-site detection of aquatic toxins produced by marine and freshwater microorganisms (cyanobacteria, dinoflagellates, and diatoms), with several advantages over other biosensors that are worth considering. Freshwater monitoring is of vital importance for public health, in numerous human activities, and animal welfare, since these toxins may cause fatal intoxications. Similarly, in marine waters, very effective monitoring programs have been put in place in many countries to detect when toxins exceed established regulatory levels and accordingly enforce shellfish harvesting closures. Recent advances in the fields of aptamer selection, nanomaterials and communication technologies, offer a vast array of possibilities to develop new imaginative strategies to create improved, ultrasensitive, reliable and real-time devices, featuring unique characteristics to produce and amplify the signal. So far, not many strategies have been used to detect aquatic toxins, mostly limited to the optic and electrochemical sensors, the majority applied to detect microcystin-LR using a target-induced switching mode. The limits of detection of these aptasensors have been decreasing from the nM to the fM order of magnitude in the past 20 years. Aspects related to sensor components, performance, aptamers sequences, matrices analyzed and future perspectives, are considered and discussed. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/120352 |
url |
https://hdl.handle.net/10216/120352 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
14243210, 14248220 10.3390/s18072367 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136154692354049 |