Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/79372 |
Resumo: | Kefiran is an exopolysaccharide produced by the microflora of kefir grains used to produce the fermented milk beverage kefir. The health-promoting and physicochemical properties of kefiran led to its exploration for a range of applications, mainly in the food industry and biomedical fields. Aiming to explore its potential for tissue engineering and regenerative medicine (TERM) applications, the kefiran biopolymer obtained through three different extraction methodologies was fully characterized and compared. High-quality kefiran polysaccharides were recovered with suitable yield through different extraction protocols. The methods consisted of heating the kefir grains prior to recovering kefiran by centrifugation and differed mainly in the precipitation steps included before lyophilization. Then, kefiran scaffolds were successfully produced from each extract by cryogelation and freeze-drying. In all extracts, it was possible to identify the molecular structure of the kefiran polysaccharide through 1H-NMR and FTIR spectra. The kefiran from extraction 1 showed the highest molecular weight (~3000 kDa) and the best rheological properties, showing a pseudoplastic behavior; its scaffold presented the highest value of porosity (93.2% ± 2), and wall thickness (85.8 µm ± 16.3). All extracts showed thermal stability, good injectability and desirable viscoelastic properties; the developed scaffolds demonstrated mechanical stability, elastic behavior, and pore size comprised between 98â 94 µm. Additionally, all kefiran products proved to be non-cytotoxic over L929 cells. The interesting structural, physicochemical, and biological properties showed by the kefiran extracts and cryogels revealed their biomedical potential and suitability for TERM applications. |
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Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicineCharacterizationExtractionKefiranRegenerative medicineScaffoldsTissue engineeringScience & TechnologyKefiran is an exopolysaccharide produced by the microflora of kefir grains used to produce the fermented milk beverage kefir. The health-promoting and physicochemical properties of kefiran led to its exploration for a range of applications, mainly in the food industry and biomedical fields. Aiming to explore its potential for tissue engineering and regenerative medicine (TERM) applications, the kefiran biopolymer obtained through three different extraction methodologies was fully characterized and compared. High-quality kefiran polysaccharides were recovered with suitable yield through different extraction protocols. The methods consisted of heating the kefir grains prior to recovering kefiran by centrifugation and differed mainly in the precipitation steps included before lyophilization. Then, kefiran scaffolds were successfully produced from each extract by cryogelation and freeze-drying. In all extracts, it was possible to identify the molecular structure of the kefiran polysaccharide through 1H-NMR and FTIR spectra. The kefiran from extraction 1 showed the highest molecular weight (~3000 kDa) and the best rheological properties, showing a pseudoplastic behavior; its scaffold presented the highest value of porosity (93.2% ± 2), and wall thickness (85.8 µm ± 16.3). All extracts showed thermal stability, good injectability and desirable viscoelastic properties; the developed scaffolds demonstrated mechanical stability, elastic behavior, and pore size comprised between 98â 94 µm. Additionally, all kefiran products proved to be non-cytotoxic over L929 cells. The interesting structural, physicochemical, and biological properties showed by the kefiran extracts and cryogels revealed their biomedical potential and suitability for TERM applications.H. Radhouani and C. Gonçalves were supported by the Foundation for Science and Technology (FCT) from Portugal, with references CEECIND/00111/2017 and SFRH/BPD/94277/2013, respectively. S. Correia and this work were funded by the R&D Project KOAT—Kefiran Exopolysaccharide: Promising Biopolymer for Use in Regenerative Medicine and Tissue Engineering, with reference PTDC/BTMMAT/29760/2017 (POCI-01-0145-FEDER-029760), financed by FCT and co-financed by FEDER and POCI, and by the Project “HEALTH-UNORTE: Setting-up biobanks and regenerative medicine strategies to boost research in cardiovascular, musculoskeletal, neurological, oncological, immunological and infectious diseases”, ref. NORTE-01-0145-FEDER-000039 funded under the program NORTE-45-2020-20—Sistema de Apoio à Investigação Científica e Tecnológica — “Projetos Estruturados de I&D&I” UNorte. We also thank Duarte N. Carvalho for input on the schematic representation of the process.MDPIUniversidade do MinhoCorreia, SusanaGonçalves, CristianaOliveira, Joaquim M.Radhouani, HajerReis, R. L.2022-062022-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/79372engCorreia S., Gonçalves C., Oliveira J. M., Radhouani H., Reis R. L. Impact of Kefiran Exopolysaccharide Extraction on Its Applicability for Tissue Engineering and Regenerative Medicine, Pharmaceutics , Vol. 14, Issue 8, pp. 1713, doi:10.3390/pharmaceutics14081713, 20221999-492310.3390/pharmaceutics14081713https://www.mdpi.com/1999-4923/14/8/1713info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:43Zoai:repositorium.sdum.uminho.pt:1822/79372Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:39.499041Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine |
title |
Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine |
spellingShingle |
Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine Correia, Susana Characterization Extraction Kefiran Regenerative medicine Scaffolds Tissue engineering Science & Technology |
title_short |
Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine |
title_full |
Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine |
title_fullStr |
Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine |
title_full_unstemmed |
Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine |
title_sort |
Impact of kefiran exopolysaccharide extraction on its applicability for tissue engineering and regenerative medicine |
author |
Correia, Susana |
author_facet |
Correia, Susana Gonçalves, Cristiana Oliveira, Joaquim M. Radhouani, Hajer Reis, R. L. |
author_role |
author |
author2 |
Gonçalves, Cristiana Oliveira, Joaquim M. Radhouani, Hajer Reis, R. L. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Correia, Susana Gonçalves, Cristiana Oliveira, Joaquim M. Radhouani, Hajer Reis, R. L. |
dc.subject.por.fl_str_mv |
Characterization Extraction Kefiran Regenerative medicine Scaffolds Tissue engineering Science & Technology |
topic |
Characterization Extraction Kefiran Regenerative medicine Scaffolds Tissue engineering Science & Technology |
description |
Kefiran is an exopolysaccharide produced by the microflora of kefir grains used to produce the fermented milk beverage kefir. The health-promoting and physicochemical properties of kefiran led to its exploration for a range of applications, mainly in the food industry and biomedical fields. Aiming to explore its potential for tissue engineering and regenerative medicine (TERM) applications, the kefiran biopolymer obtained through three different extraction methodologies was fully characterized and compared. High-quality kefiran polysaccharides were recovered with suitable yield through different extraction protocols. The methods consisted of heating the kefir grains prior to recovering kefiran by centrifugation and differed mainly in the precipitation steps included before lyophilization. Then, kefiran scaffolds were successfully produced from each extract by cryogelation and freeze-drying. In all extracts, it was possible to identify the molecular structure of the kefiran polysaccharide through 1H-NMR and FTIR spectra. The kefiran from extraction 1 showed the highest molecular weight (~3000 kDa) and the best rheological properties, showing a pseudoplastic behavior; its scaffold presented the highest value of porosity (93.2% ± 2), and wall thickness (85.8 µm ± 16.3). All extracts showed thermal stability, good injectability and desirable viscoelastic properties; the developed scaffolds demonstrated mechanical stability, elastic behavior, and pore size comprised between 98â 94 µm. Additionally, all kefiran products proved to be non-cytotoxic over L929 cells. The interesting structural, physicochemical, and biological properties showed by the kefiran extracts and cryogels revealed their biomedical potential and suitability for TERM applications. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06 2022-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/79372 |
url |
https://hdl.handle.net/1822/79372 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Correia S., Gonçalves C., Oliveira J. M., Radhouani H., Reis R. L. Impact of Kefiran Exopolysaccharide Extraction on Its Applicability for Tissue Engineering and Regenerative Medicine, Pharmaceutics , Vol. 14, Issue 8, pp. 1713, doi:10.3390/pharmaceutics14081713, 2022 1999-4923 10.3390/pharmaceutics14081713 https://www.mdpi.com/1999-4923/14/8/1713 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
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MDPI |
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