Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis

Detalhes bibliográficos
Autor(a) principal: Doan, T.
Data de Publicação: 2009
Outros Autores: Morlot, C., Meisner, J., Serrano, M., Henriques, A. O., Moran, C. P., Rudner, D. Z.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/5842
Resumo: Plos Genetics, 5(7): ARTe1000566
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spelling Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilisBACTERIAL ENDOSPORE FORMATIONSPORULATION MEMBRANE-PROTEINTRANSCRIPTION FACTORSUBCELLULAR-LOCALIZATIONSIGMA-FACTORSENGULFMENTFORESPORECELLLOCUSMECHANISMPlos Genetics, 5(7): ARTe1000566Sporulation in Bacillus subtilis involves two cells that follow separate but coordinately regulated developmental programs. Late in sporulation, the developing spore (the forespore) resides within a mother cell. The regulation of the forespore transcription factor sigma(G) that acts at this stage has remained enigmatic. sigma(G) activity requires eight mother-cell proteins encoded in the spoIIIA operon and the forespore protein SpoIIQ. Several of the SpoIIIA proteins share similarity with components of specialized secretion systems. One of them resembles a secretion ATPase and we demonstrate that the ATPase motifs are required for sigma(G) activity. We further show that the SpoIIIA proteins and SpoIIQ reside in a multimeric complex that spans the two membranes surrounding the forespore. Finally, we have discovered that these proteins are all required to maintain forespore integrity. In their absence, the forespore develops large invaginations and collapses. Importantly, maintenance of forespore integrity does not require sigma(G). These results support a model in which the SpoIIIA- SpoIIQ proteins form a novel secretion apparatus that allows the mother cell to nurture the forespore, thereby maintaining forespore physiology and sigma(G) activity during spore maturation.Public Library of Science (PLoS)RUNDoan, T.Morlot, C.Meisner, J.Serrano, M.Henriques, A. O.Moran, C. P.Rudner, D. Z.2011-06-27T14:22:57Z2009-01-01T00:00:00Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/5842eng1553-7390info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-10T15:25:42ZPortal AgregadorONG
dc.title.none.fl_str_mv Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
title Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
spellingShingle Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
Doan, T.
BACTERIAL ENDOSPORE FORMATION
SPORULATION MEMBRANE-PROTEIN
TRANSCRIPTION FACTOR
SUBCELLULAR-LOCALIZATION
SIGMA-FACTORS
ENGULFMENT
FORESPORE
CELL
LOCUS
MECHANISM
title_short Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
title_full Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
title_fullStr Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
title_full_unstemmed Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
title_sort Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis
author Doan, T.
author_facet Doan, T.
Morlot, C.
Meisner, J.
Serrano, M.
Henriques, A. O.
Moran, C. P.
Rudner, D. Z.
author_role author
author2 Morlot, C.
Meisner, J.
Serrano, M.
Henriques, A. O.
Moran, C. P.
Rudner, D. Z.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Doan, T.
Morlot, C.
Meisner, J.
Serrano, M.
Henriques, A. O.
Moran, C. P.
Rudner, D. Z.
dc.subject.por.fl_str_mv BACTERIAL ENDOSPORE FORMATION
SPORULATION MEMBRANE-PROTEIN
TRANSCRIPTION FACTOR
SUBCELLULAR-LOCALIZATION
SIGMA-FACTORS
ENGULFMENT
FORESPORE
CELL
LOCUS
MECHANISM
topic BACTERIAL ENDOSPORE FORMATION
SPORULATION MEMBRANE-PROTEIN
TRANSCRIPTION FACTOR
SUBCELLULAR-LOCALIZATION
SIGMA-FACTORS
ENGULFMENT
FORESPORE
CELL
LOCUS
MECHANISM
description Plos Genetics, 5(7): ARTe1000566
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01T00:00:00Z
2009-01-01
2011-06-27T14:22:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/5842
url http://hdl.handle.net/10362/5842
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1553-7390
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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