Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/130111 |
Resumo: | "Anaplastic thyroid cancer (ATC) is the most aggressive form of thyroid cancer, with very high mortality rate. Tumor-associated macrophages (TAMs) can represent up to 70% of ATC’s tumor mass, making them an interesting target for novel therapies. In this thesis, the aim was to further understand the crosstalk between ATC and TAMs. To achieve that, cell surface proteomics of two ATC cell lines (C3948 and T235), in mono- or co-culture with THP-1-derived macrophage-like cells, was performed. The protein Spry-4, an inhibitor of the MAPK-ERK pathway, was found to be downregulated in C3948 cells upon co-culture. Spry-4 protein levels were further evaluated by Western blot; only T235 in co-culture showed a trend for downregulation, although without statistical significance.(...)" |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Exploring Tumor Macrophage Interaction in Anaplastic Thyroid CancerAnaplastic Thyroid CancerTumor-associated MacrophagesSpry-43D Cell ModelsTumor Microenvironment"Anaplastic thyroid cancer (ATC) is the most aggressive form of thyroid cancer, with very high mortality rate. Tumor-associated macrophages (TAMs) can represent up to 70% of ATC’s tumor mass, making them an interesting target for novel therapies. In this thesis, the aim was to further understand the crosstalk between ATC and TAMs. To achieve that, cell surface proteomics of two ATC cell lines (C3948 and T235), in mono- or co-culture with THP-1-derived macrophage-like cells, was performed. The protein Spry-4, an inhibitor of the MAPK-ERK pathway, was found to be downregulated in C3948 cells upon co-culture. Spry-4 protein levels were further evaluated by Western blot; only T235 in co-culture showed a trend for downregulation, although without statistical significance.(...)"Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António XavierRUNEduardo, Rodrigo2022-11-30T01:33:13Z2019-112019-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/130111enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:57:49Zoai:run.unl.pt:10362/130111Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:57:49Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer |
title |
Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer |
spellingShingle |
Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer Eduardo, Rodrigo Anaplastic Thyroid Cancer Tumor-associated Macrophages Spry-4 3D Cell Models Tumor Microenvironment |
title_short |
Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer |
title_full |
Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer |
title_fullStr |
Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer |
title_full_unstemmed |
Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer |
title_sort |
Exploring Tumor Macrophage Interaction in Anaplastic Thyroid Cancer |
author |
Eduardo, Rodrigo |
author_facet |
Eduardo, Rodrigo |
author_role |
author |
dc.contributor.none.fl_str_mv |
RUN |
dc.contributor.author.fl_str_mv |
Eduardo, Rodrigo |
dc.subject.por.fl_str_mv |
Anaplastic Thyroid Cancer Tumor-associated Macrophages Spry-4 3D Cell Models Tumor Microenvironment |
topic |
Anaplastic Thyroid Cancer Tumor-associated Macrophages Spry-4 3D Cell Models Tumor Microenvironment |
description |
"Anaplastic thyroid cancer (ATC) is the most aggressive form of thyroid cancer, with very high mortality rate. Tumor-associated macrophages (TAMs) can represent up to 70% of ATC’s tumor mass, making them an interesting target for novel therapies. In this thesis, the aim was to further understand the crosstalk between ATC and TAMs. To achieve that, cell surface proteomics of two ATC cell lines (C3948 and T235), in mono- or co-culture with THP-1-derived macrophage-like cells, was performed. The protein Spry-4, an inhibitor of the MAPK-ERK pathway, was found to be downregulated in C3948 cells upon co-culture. Spry-4 protein levels were further evaluated by Western blot; only T235 in co-culture showed a trend for downregulation, although without statistical significance.(...)" |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11 2019-11-01T00:00:00Z 2022-11-30T01:33:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/130111 |
url |
http://hdl.handle.net/10362/130111 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier |
publisher.none.fl_str_mv |
Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817545834925916160 |