Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing

Detalhes bibliográficos
Autor(a) principal: Garelli, Andres
Data de Publicação: 2015
Outros Autores: Heredia, Fabiana, Casimiro, Andreia P., Macedo, Andre, Nunes, Catarina, Garcez, Marcia, Dias, Angela R.Mantas, Volonte, Yanel A., Uhlmann, Thomas, Caparros, Esther, Koyama, Takashi, Gontijo, Alisson M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/152656
Resumo: Funding Information: We thank C. Mirth, C. Ribeiro and A. Jacinto for their comments and suggestions; I. Miguel-Aliaga, A. Jacinto, P. Leopold, P. Domingos, C. Mirth, R. Teodoro, M. Dominguez, M.L. Vasconcelos, J.C. Yin and M. O’Connor, for reagents. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. A.M.G., F.H., A.M., A.R.M.D. and T.K. are supported by the FCT, under the FCT Investigator Programme and FCT fellowships SFRH/BPD/94112/ 2013, PD/BD/52421/2013, SFRH/BD/94931/2013 and SFRH/BPD/74313/2010, respectively. A.G. is supported by the CONICET and UNS, and Y.A.V. holds a CONICET fellowship. The work in the laboratory of A.M.G. is funded by the CEDOC and the European Commission FP7 (PCIG13-GA-2013-618847). A.G. thanks N.P. Rotstein and L.E. Politi for providing funds and space to develop a part of this project in their lab. Publisher Copyright: © 2015 Macmillan Publishers Limited All rights reserved.
id RCAP_419044ca2bdf849e2b061bd11c64665e
oai_identifier_str oai:run.unl.pt:10362/152656
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timingChemistry(all)Biochemistry, Genetics and Molecular Biology(all)GeneralPhysics and Astronomy(all)Funding Information: We thank C. Mirth, C. Ribeiro and A. Jacinto for their comments and suggestions; I. Miguel-Aliaga, A. Jacinto, P. Leopold, P. Domingos, C. Mirth, R. Teodoro, M. Dominguez, M.L. Vasconcelos, J.C. Yin and M. O’Connor, for reagents. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. A.M.G., F.H., A.M., A.R.M.D. and T.K. are supported by the FCT, under the FCT Investigator Programme and FCT fellowships SFRH/BPD/94112/ 2013, PD/BD/52421/2013, SFRH/BD/94931/2013 and SFRH/BPD/74313/2010, respectively. A.G. is supported by the CONICET and UNS, and Y.A.V. holds a CONICET fellowship. The work in the laboratory of A.M.G. is funded by the CEDOC and the European Commission FP7 (PCIG13-GA-2013-618847). A.G. thanks N.P. Rotstein and L.E. Politi for providing funds and space to develop a part of this project in their lab. Publisher Copyright: © 2015 Macmillan Publishers Limited All rights reserved.How different organs in the body sense growth perturbations in distant tissues to coordinate their size during development is poorly understood. Here we mutate an invertebrate orphan relaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3 (Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8) mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNA intereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginal disc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 and performing cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons, four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respond specifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds new light on the function and evolution of relaxin receptors and reveals a novel neuroendocrine circuit responsive to growth aberrations.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNGarelli, AndresHeredia, FabianaCasimiro, Andreia P.Macedo, AndreNunes, CatarinaGarcez, MarciaDias, Angela R.MantasVolonte, Yanel A.Uhlmann, ThomasCaparros, EstherKoyama, TakashiGontijo, Alisson M.2023-05-11T22:13:59Z2015-10-292015-10-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/152656eng2041-1723PURE: 60373814https://doi.org/10.1038/ncomms9732info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:11:22Zoai:run.unl.pt:10362/152656Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:11:22Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
spellingShingle Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
Garelli, Andres
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)
title_short Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title_full Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title_fullStr Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title_full_unstemmed Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
title_sort Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing
author Garelli, Andres
author_facet Garelli, Andres
Heredia, Fabiana
Casimiro, Andreia P.
Macedo, Andre
Nunes, Catarina
Garcez, Marcia
Dias, Angela R.Mantas
Volonte, Yanel A.
Uhlmann, Thomas
Caparros, Esther
Koyama, Takashi
Gontijo, Alisson M.
author_role author
author2 Heredia, Fabiana
Casimiro, Andreia P.
Macedo, Andre
Nunes, Catarina
Garcez, Marcia
Dias, Angela R.Mantas
Volonte, Yanel A.
Uhlmann, Thomas
Caparros, Esther
Koyama, Takashi
Gontijo, Alisson M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Garelli, Andres
Heredia, Fabiana
Casimiro, Andreia P.
Macedo, Andre
Nunes, Catarina
Garcez, Marcia
Dias, Angela R.Mantas
Volonte, Yanel A.
Uhlmann, Thomas
Caparros, Esther
Koyama, Takashi
Gontijo, Alisson M.
dc.subject.por.fl_str_mv Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)
topic Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)
description Funding Information: We thank C. Mirth, C. Ribeiro and A. Jacinto for their comments and suggestions; I. Miguel-Aliaga, A. Jacinto, P. Leopold, P. Domingos, C. Mirth, R. Teodoro, M. Dominguez, M.L. Vasconcelos, J.C. Yin and M. O’Connor, for reagents. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. A.M.G., F.H., A.M., A.R.M.D. and T.K. are supported by the FCT, under the FCT Investigator Programme and FCT fellowships SFRH/BPD/94112/ 2013, PD/BD/52421/2013, SFRH/BD/94931/2013 and SFRH/BPD/74313/2010, respectively. A.G. is supported by the CONICET and UNS, and Y.A.V. holds a CONICET fellowship. The work in the laboratory of A.M.G. is funded by the CEDOC and the European Commission FP7 (PCIG13-GA-2013-618847). A.G. thanks N.P. Rotstein and L.E. Politi for providing funds and space to develop a part of this project in their lab. Publisher Copyright: © 2015 Macmillan Publishers Limited All rights reserved.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-29
2015-10-29T00:00:00Z
2023-05-11T22:13:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/152656
url http://hdl.handle.net/10362/152656
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2041-1723
PURE: 60373814
https://doi.org/10.1038/ncomms9732
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817545932656345088

Registros relacionados