CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish

Detalhes bibliográficos
Autor(a) principal: Amorim, JP
Data de Publicação: 2020
Outros Autores: Bordeira-Carriço, R, Gali-Macedo, A, Perrod, C, Bessa, J
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/145275
Resumo: Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRNA, followed by microinjection in zebrafish embryos and subsequent genotype screening for the establishment of a mutant line. The versatility and efficiency of this pipeline makes the generation of zebrafish models a widely used approach in functional genetics. For complete details on the use and execution of this protocol, please refer to Amorim et al. (2020).
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spelling CRISPR-Cas9-Mediated Genomic Deletions Protocol in ZebrafishCRISPRGeneticsGenomicsModel OrganismsSince its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRNA, followed by microinjection in zebrafish embryos and subsequent genotype screening for the establishment of a mutant line. The versatility and efficiency of this pipeline makes the generation of zebrafish models a widely used approach in functional genetics. For complete details on the use and execution of this protocol, please refer to Amorim et al. (2020).Cell Press20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/145275eng2666-166710.1016/j.xpro.2020.100208Amorim, JPBordeira-Carriço, RGali-Macedo, APerrod, CBessa, Jinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:44:39Zoai:repositorio-aberto.up.pt:10216/145275Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:30:58.084100Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
title CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
spellingShingle CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
Amorim, JP
CRISPR
Genetics
Genomics
Model Organisms
title_short CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
title_full CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
title_fullStr CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
title_full_unstemmed CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
title_sort CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
author Amorim, JP
author_facet Amorim, JP
Bordeira-Carriço, R
Gali-Macedo, A
Perrod, C
Bessa, J
author_role author
author2 Bordeira-Carriço, R
Gali-Macedo, A
Perrod, C
Bessa, J
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Amorim, JP
Bordeira-Carriço, R
Gali-Macedo, A
Perrod, C
Bessa, J
dc.subject.por.fl_str_mv CRISPR
Genetics
Genomics
Model Organisms
topic CRISPR
Genetics
Genomics
Model Organisms
description Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRNA, followed by microinjection in zebrafish embryos and subsequent genotype screening for the establishment of a mutant line. The versatility and efficiency of this pipeline makes the generation of zebrafish models a widely used approach in functional genetics. For complete details on the use and execution of this protocol, please refer to Amorim et al. (2020).
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/145275
url https://hdl.handle.net/10216/145275
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2666-1667
10.1016/j.xpro.2020.100208
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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